Difference between revisions of "Team:Tacoma RAINmakers/Safety"

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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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RAINmakers are employing 5-alpha competent E. coli cells (DH5-Alpha strain). According to the Globally Harmonized Systems (GHS), this organism is not a dangerous substance or mixture. Per Centers for Disease Control and Prevention (CDC), this organism can be handled at Biology Safety Level One (BSL-1). The CDC goes on to state that 5-alpha competent E. coli cells present no risk of disease in immunocompetent adult humans and no hazard to laboratory personnel and the environment. There are certain first aid measures that must be taken if the 5-alpha E. coli cells come in contact with lab personnel. If the cells come in contact with skin, washing with soap and water is sufficient. If there is eye contact, rising thoroughly with water for 15 minutes is imperative, as well as consultation of a physician. In the case of inhalation, exposure to fresh air is all that is necessary. All members of Tacoma RAINmakers are versed in these simple first aid measures. In the case of accidental release into the environment, cells should be soaked up with inert absorbent material, transferred to a properly labelled container, and disposed of as hazardous waste. Still, it is important to recall that this organism presents no environmental threat.
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<li>In lab, the first step for Tacoma RAINmakers is cloning our construct. We digested our vector, ArsR gene, and chromoproteins. Since ArsR and our chromoprotein make up the insert portion of the construct, they must be ligated. Then, the newly created insert is ligated into our vector, forming our full construct. The construct DNA is added to 5-alpha competent E. coli cells and grown in LB broth. At this point, the RAINmakers would "mini-prep" our E. coli cells using cell-free lysate kits. Now that the desired DNA material has been produced, we are able to proceed to our experiments. One such experiment will involve pipetting our circuit DNA into our paper ticket, exposing the ticket to arsenic solution, incubating, and qualitatively assessing chromoprotein production. Specific aspects of this experiments that must be optimized include incubation time, DNA quantity, and reagent proportions. Additionally, RAINmakers will empirically determine the maximum and minimum amount of arsenic concentration our biosensor is able to detect. Once our sensing mechanism is functional with known quantities of arsenic, we will test our ticket with environmental samples (i.e. water, diluted soil). All parts mentioned are already in the registry.
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<li> worm nitrile rubber gloves that equal or exceed required layer thickness (0.11mm)</li>
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<li>Contaminated gloves were disposed of with proper glove removal technique (without touching glove's outer surface).</li>
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<li>To protect the body, our team worn lab coats during all arsenic-related experiments.</li>
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<li>all experiments involving arsenic took place within a chemistry fume hood if any aerosolizing is to occur.</li>
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<li>Arsenic solutions or powder will not be allowed to enter drains or any location outside the lab.</li>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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            <h1>Safety Procedures</h1>
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<li class="listy"> Lab techs wore nitrile gloves to protect themselves, and to prevent contamination of samples. All lab waste was disposed of properly, through decontamination procedures (i.e. autoclave, disinfectant, etc.) or hazardous waste disposal.</li>
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<li class="listy"> We followed the strict rule to not eat and drink in the lab. Food was always left on the shelf in front of the lab to prevent the accidental ingestion of chemicals.</li>
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<li class="listy"> Any team members wearing shorts, or working with dangerous materials, wore lab coats. This protected them against accidental chemical spills.</li>
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<li class="listy"> The lab sub-team leader, Keshava, taught team members how to properly use the equipment. He had participated in iGEM on the 2017 Cadets2Vets team, so he was a great resource to learn about the safety features of lab tools!</li>
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<h3>Safe Lab Work</h3>
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<p>The most significant hazard in our project was the use of chemicals containing arsenic.</p>
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<li class="listy">  Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life, with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, we used sodium arsenate and sodium arsenite. They are the inorganic derivatives of arsenic and have been shown to be significantly less toxic than pure arsenic.</li>
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<li class="listy"> When using sodium arsenate/sodium arsenite, we engaged in a series of stringent safety practices. Safety glasses were worn to protect eyes. For hand protection, our team wore nitrile gloves equal to, or exceeding the required layer thickness (0.11mm). Contaminated gloves were disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team wore lab coats during all arsenic-related experiments. We considered the use of air-purifying respirators but deemed this precaution unnecessary as we used a biosafety cabinet when working with sodium arsenate/sodium arsenite. Arsenic waste solutions and powders did not enter drains or any location outside the lab.</li>
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Latest revision as of 18:57, 11 December 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook

Safety Procedures


  • Lab techs wore nitrile gloves to protect themselves, and to prevent contamination of samples. All lab waste was disposed of properly, through decontamination procedures (i.e. autoclave, disinfectant, etc.) or hazardous waste disposal.
  • We followed the strict rule to not eat and drink in the lab. Food was always left on the shelf in front of the lab to prevent the accidental ingestion of chemicals.
  • Any team members wearing shorts, or working with dangerous materials, wore lab coats. This protected them against accidental chemical spills.
  • The lab sub-team leader, Keshava, taught team members how to properly use the equipment. He had participated in iGEM on the 2017 Cadets2Vets team, so he was a great resource to learn about the safety features of lab tools!

    • Safe Lab Work


    The most significant hazard in our project was the use of chemicals containing arsenic.

  • Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life, with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, we used sodium arsenate and sodium arsenite. They are the inorganic derivatives of arsenic and have been shown to be significantly less toxic than pure arsenic.
  • When using sodium arsenate/sodium arsenite, we engaged in a series of stringent safety practices. Safety glasses were worn to protect eyes. For hand protection, our team wore nitrile gloves equal to, or exceeding the required layer thickness (0.11mm). Contaminated gloves were disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team wore lab coats during all arsenic-related experiments. We considered the use of air-purifying respirators but deemed this precaution unnecessary as we used a biosafety cabinet when working with sodium arsenate/sodium arsenite. Arsenic waste solutions and powders did not enter drains or any location outside the lab.