Difference between revisions of "Team:Tacoma RAINmakers/Safety"

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            <h1>Safety Procedures</h1>
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<li> Starting from the left, we always used nitrile gloves that protect us and prevent contamination of chemicals. Any lab materials were disposed of properly through decontamination procedures or hazardous waste disposal.</li>
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<li>Tacoma_RAINmakers also followed the strict rule to not eat and drink in the lab. Food were always left behind on the shelf in front of the lab, and we followed this practice to accidental ingestion of chemicals.</li>
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<li>Tacoma_RAINmakers wore labcoats when they were wearing shorts. This protected us against accidental chemical spills.</li>
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<li>One of our subteam leaders, Keshava, taught our current team members about how to run lab equipment. He had participated iGem in previous year, so he was a great resource to learn about the safety features of lab tools!</li>
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<li class="listy"> Lab techs wore nitrile gloves to protect themselves, and to prevent contamination of samples. All lab waste was disposed of properly, through decontamination procedures (i.e. autoclave, disinfectant, etc.) or hazardous waste disposal.</li>
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<li class="listy"> We followed the strict rule to not eat and drink in the lab. Food was always left on the shelf in front of the lab to prevent the accidental ingestion of chemicals.</li>
 +
<li class="listy"> Any team members wearing shorts, or working with dangerous materials, wore lab coats. This protected them against accidental chemical spills.</li>
 +
<li class="listy"> The lab sub-team leader, Keshava, taught team members how to properly use the equipment. He had participated in iGEM on the 2017 Cadets2Vets team, so he was a great resource to learn about the safety features of lab tools!</li>
  
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<h3>Safe Lab Work</h3>
 
<h3>Safe Lab Work</h3>
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<p>The most significant hazard in the RAINmaker project would be the use of chemicals containing arsenic.</p>
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<li>Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, Tacoma RAINmakers used the sodium arsenate and sodium arsenite, which are the inorganic derivatives of arsenic that shows significantly less toxicity than the pure arsenic.</li>
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<li>When using sodium arsenate/sodium arsenite, Tacoma RAINmakers are engaging in a series of stringent safety practices. First, safety glasses will always be used to protect eyes. For hand protection, our team will wear nitrile gloves that equal or exceed required layer thickness (0.11mm). Contaminated gloves will be disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team will wear lab coats during all arsenic-related experiments. RAINmakers have considered air-purifying respirators, but have deemed this precaution unnecessary with proper lab technique use of a biosafety cabinet. Arsenic waste solutions or powder will not be allowed to enter drains or any location outside the lab.</li>
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<p>The most significant hazard in our project was the use of chemicals containing arsenic.</p>
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<li class="listy"> Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life, with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, we used sodium arsenate and sodium arsenite. They are the inorganic derivatives of arsenic and have been shown to be significantly less toxic than pure arsenic.</li>
 +
<li class="listy"> When using sodium arsenate/sodium arsenite, we engaged in a series of stringent safety practices. Safety glasses were worn to protect eyes. For hand protection, our team wore nitrile gloves equal to, or exceeding the required layer thickness (0.11mm). Contaminated gloves were disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team wore lab coats during all arsenic-related experiments. We considered the use of air-purifying respirators but deemed this precaution unnecessary as we used a biosafety cabinet when working with sodium arsenate/sodium arsenite. Arsenic waste solutions and powders did not enter drains or any location outside the lab.</li>
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Latest revision as of 18:57, 11 December 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook

Safety Procedures


  • Lab techs wore nitrile gloves to protect themselves, and to prevent contamination of samples. All lab waste was disposed of properly, through decontamination procedures (i.e. autoclave, disinfectant, etc.) or hazardous waste disposal.
  • We followed the strict rule to not eat and drink in the lab. Food was always left on the shelf in front of the lab to prevent the accidental ingestion of chemicals.
  • Any team members wearing shorts, or working with dangerous materials, wore lab coats. This protected them against accidental chemical spills.
  • The lab sub-team leader, Keshava, taught team members how to properly use the equipment. He had participated in iGEM on the 2017 Cadets2Vets team, so he was a great resource to learn about the safety features of lab tools!

    • Safe Lab Work


    The most significant hazard in our project was the use of chemicals containing arsenic.

  • Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life, with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, we used sodium arsenate and sodium arsenite. They are the inorganic derivatives of arsenic and have been shown to be significantly less toxic than pure arsenic.
  • When using sodium arsenate/sodium arsenite, we engaged in a series of stringent safety practices. Safety glasses were worn to protect eyes. For hand protection, our team wore nitrile gloves equal to, or exceeding the required layer thickness (0.11mm). Contaminated gloves were disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team wore lab coats during all arsenic-related experiments. We considered the use of air-purifying respirators but deemed this precaution unnecessary as we used a biosafety cabinet when working with sodium arsenate/sodium arsenite. Arsenic waste solutions and powders did not enter drains or any location outside the lab.