Difference between revisions of "Team:NUS Singapore-A"

 
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    <h2 id='Overview'>Coup Dy'état: Eco-friendly Bio-manufacturing of Flavonoid Dyes in Escherichia coli via Computer-mediated Optogenetic Regulation</h2>
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<img src="https://static.igem.org/mediawiki/2018/3/38/T--NUS_Singapore-A--the_main_header_gais.png">
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<img src="https://static.igem.org/mediawiki/2018/8/8d/T--NUS_Singapore-A--FrontPage_Trans2.png" class="main-page-img">
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<div class="text-box-description">Biomanufacturing opens up possibilities for sustainable <br>production of useful materials. <br><br>
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We, <b>Team NUSGEM</b>, created <font class="font-class-12"><b><i>Coup Dy’état</i></b></font>,<br> <font class="font-class-5">an environmentally-friendly platform to advance biomanufacturing.</font><br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Description"><b>Discover our project</b></a>
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  </div>
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  <div class="text-box-design">Current biomanufacturing platforms are <br>chemical-intensive and expensive. <br><br>
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<b><i>Coup Dy’état</i></b> is not only <font class="font-class-6"><b>environmentally-friendly</b></font>, but also potentially <font class="font-class-6"><b>cheaper</b></font>. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Design"><b>Find out how we designed our platform</b></a>
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</div>
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  <div class="text-box-ihp">Integrating findings from our human practices has helped us make sure that our platform is <font class="font-class-6"><b>relevant</b></font> and <font class="font-class-6"><b>useful</b></font> to biomanufacturers. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Human_Practices"><b>Find out who we spoke to</b></a></div>
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  <div class="text-box-results">
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<br><br> To prove that our platform works, we decided to produce flavonoids to be used as dyes.<br><br>
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This is because the textile dyeing and dyestuff production industry is the <i>second most pollutive industry in the world</i>. <br><br>
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Our biomanufacturing platform can <br> <font class="font-class-10"><b>help</b></font>.<br><br>
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<a href="https://2018.igem.org/Team:NUS_Singapore-A/Results"><b>See our results</b></a></div>
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  <div class="text-box-modeling">No system would work without its <font class="font-class-6"><b>support</b></font>.<br><br> Find out how we <a href="https://2018.igem.org/Team:NUS_Singapore-A/Model"><b>modelled</b></a> our complete system and how we built our <a href="https://2018.igem.org/Team:NUS_Singapore-A/Hardware"><b>hardware</b></a> system to support our platform.</div>
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  <div class="text-box-team">Our team of 13 is made up <font class="font-class-6"><b><i>intensely</i> motivated</b></font> students across all disciplines. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Team"><b>Find out who we are</b></a></div>
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<font class="font-class-9"><center><b>Our Achievements</b></center></font>
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<br>
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<a target="_blank" href="https://www.eng.nus.edu.sg/2018/gold-for-nus-igem-team-2018/"><img src="https://static.igem.org/mediawiki/2018/c/cc/T--NUS_Singapore-A--Group_Photo.jpeg"></a>
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<br>
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  <figcaption><b>Here's a lovely photo of us, flush with post-Jamboree endorphins! Click to read more on our university's website.</figcaption>
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<br>
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<p>We are thrilled to announce that <i>Coup Dy’état</i> has won a <b>Gold Medal</b> and obtained 3 nominations for special awards: <b>Best Manufacturing Project, Best Hardware</b>, and the <b>Safety Commendation</b>. Thank you for being with us on our iGEM journey!</p><br><br>
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    <p style="margin: 0 5%"> Synthetic clothing dyes are a major source of water pollution. Natural dyes are more beneficial to the environment, but production is land-intensive and low-yield. To increase the viability of natural dyes as an alternative to synthetic dyes, we developed a novel, eco-friendly method for the biomanufacturing of a natural flavonoid dye, luteolin, in E. coli. We performed mutagenesis for more efficient xylose metabolization, allowing us to use lignocellulosic waste as feedstock. Also, we designed an automated closed-loop cell-computer interface to enhance production through dynamic regulation and stress regulation. The system obtains feedback from an optical density sensor and a stress-sensing fluorescent reporter. As we control gene expression with a blue light repressible promoter, blue LEDs activate and deactivate accordingly to maintain an optimal rate of production, eliminating the need for chemical inducers. We envision that our system has great potential for finer control and optimization as more functions are incorporated.
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<font class="font-class-9"><center><b>Join us in our vision for the <br><br> Biomanufacturing of the Future.</b></center></font><br>
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Latest revision as of 01:47, 14 December 2018

CONNECT WITH US

Biomanufacturing opens up possibilities for sustainable
production of useful materials.

We, Team NUSGEM, created Coup Dy’état,
an environmentally-friendly platform to advance biomanufacturing.

Discover our project
Current biomanufacturing platforms are
chemical-intensive and expensive.

Coup Dy’état is not only environmentally-friendly, but also potentially cheaper.

Find out how we designed our platform
Integrating findings from our human practices has helped us make sure that our platform is relevant and useful to biomanufacturers.

Find out who we spoke to


To prove that our platform works, we decided to produce flavonoids to be used as dyes.

This is because the textile dyeing and dyestuff production industry is the second most pollutive industry in the world.

Our biomanufacturing platform can
help.

See our results
No system would work without its support.

Find out how we modelled our complete system and how we built our hardware system to support our platform.
Our team of 13 is made up intensely motivated students across all disciplines.

Find out who we are
Our Achievements

Here's a lovely photo of us, flush with post-Jamboree endorphins! Click to read more on our university's website.

We are thrilled to announce that Coup Dy’état has won a Gold Medal and obtained 3 nominations for special awards: Best Manufacturing Project, Best Hardware, and the Safety Commendation. Thank you for being with us on our iGEM journey!



Join us in our vision for the

Biomanufacturing of the Future.



HEAR OUR STORY

We’re using synthetic biology to change the way the world thinks and functions.

We’re a little disruptive, dangerous and maybe a tad too bold. But the world needs some shaking up every now and then, and we think we’re just the right people for that.

Together we’re going to engineer biology and create something awesome.

CONTACT US

nusigem@gmail.com

21 Lower Kent Ridge Rd, Singapore 119077