Difference between revisions of "Team:NUS Singapore-A"

 
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    <h2 id='Overview'>Eco-friendly Bio-manufacturing of Flavonoid Dyes in Escherichia coli via Computer-mediated Optogenetic Regulation</h2>
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<img src="https://static.igem.org/mediawiki/2018/3/38/T--NUS_Singapore-A--the_main_header_gais.png">
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<img src="https://static.igem.org/mediawiki/2018/8/8d/T--NUS_Singapore-A--FrontPage_Trans2.png" class="main-page-img">
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<div class="text-box-description">Biomanufacturing opens up possibilities for sustainable <br>production of useful materials. <br><br>
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We, <b>Team NUSGEM</b>, created <font class="font-class-12"><b><i>Coup Dy’état</i></b></font>,<br> <font class="font-class-5">an environmentally-friendly platform to advance biomanufacturing.</font><br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Description"><b>Discover our project</b></a>
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   </div>
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  <div class="text-box-design">Current biomanufacturing platforms are <br>chemical-intensive and expensive. <br><br>
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<b><i>Coup Dy’état</i></b> is not only <font class="font-class-6"><b>environmentally-friendly</b></font>, but also potentially <font class="font-class-6"><b>cheaper</b></font>. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Design"><b>Find out how we designed our platform</b></a>
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</div>
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  <div class="text-box-ihp">Integrating findings from our human practices has helped us make sure that our platform is <font class="font-class-6"><b>relevant</b></font> and <font class="font-class-6"><b>useful</b></font> to biomanufacturers. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Human_Practices"><b>Find out who we spoke to</b></a></div>
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  <div class="text-box-results">
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<br><br> To prove that our platform works, we decided to produce flavonoids to be used as dyes.<br><br>
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This is because the textile dyeing and dyestuff production industry is the <i>second most pollutive industry in the world</i>. <br><br>
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Our biomanufacturing platform can <br> <font class="font-class-10"><b>help</b></font>.<br><br>
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<a href="https://2018.igem.org/Team:NUS_Singapore-A/Results"><b>See our results</b></a></div>
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  <div class="text-box-modeling">No system would work without its <font class="font-class-6"><b>support</b></font>.<br><br> Find out how we <a href="https://2018.igem.org/Team:NUS_Singapore-A/Model"><b>modelled</b></a> our complete system and how we built our <a href="https://2018.igem.org/Team:NUS_Singapore-A/Hardware"><b>hardware</b></a> system to support our platform.</div>
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  <div class="text-box-team">Our team of 13 is made up <font class="font-class-6"><b><i>intensely</i> motivated</b></font> students across all disciplines. <br><br><a href="https://2018.igem.org/Team:NUS_Singapore-A/Team"><b>Find out who we are</b></a></div>
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    <p style="margin: 0 5%"> Natural dyes are increasingly considered as an eco-friendly solution to the serious water pollution generated by the textile and dye industries. Traditional production of natural dyes from plants heavily exhausts land and labour. While bio-manufacturing is an attractive alternative, it remains costly and chemically-intensive. We aim to develop a new bio-manufacturing method of producing flavonoids in E. coli for use as natural dyes. To eliminate the use of expensive chemical inducers to switch from growth to production phase and allow dynamic gene regulation, we designed an optogenetic circuit using a blue light repressible promoter for flavonoid biosynthesis. As it is critical to monitor cellular metabolic burden for efficient production, we introduced a stress-sensing fluorescence reporter. To optimize operations, a computer-aided system was developed to regulate gene expression using light according to the feedback from the stress sensor. To demonstrate this approach, we produced Luteolin, a natural yellow dye.
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<font class="font-class-9"><center><b>Our Achievements</b></center></font>
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<br>
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<a target="_blank" href="https://www.eng.nus.edu.sg/2018/gold-for-nus-igem-team-2018/"><img src="https://static.igem.org/mediawiki/2018/c/cc/T--NUS_Singapore-A--Group_Photo.jpeg"></a>
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  <figcaption><b>Here's a lovely photo of us, flush with post-Jamboree endorphins! Click to read more on our university's website.</figcaption>
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<h2 id='Overview'> The Problem</h2>
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<p>We are thrilled to announce that <i>Coup Dy’état</i> has won a <b>Gold Medal</b> and obtained 3 nominations for special awards: <b>Best Manufacturing Project, Best Hardware</b>, and the <b>Safety Commendation</b>. Thank you for being with us on our iGEM journey!</p><br><br>
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    <p style="margin: 0 5%">Water pollution is a key problem in the textile dyeing and dyestuff production industry. Drinking water flows out of our faucets in Singapore, which we sometimes take for granted. However, in many parts of the world, rivers and waterbodies that are sources of drinking water and livelihood are becoming heavily polluted by textile dyeing. Due to irresponsible disposal of industrial effluents, hard-to-biodegrade synthetic dyes by textiles and dyestuff producers and ineffective wastewater treatment, chemicals accumulate and make these rivers and waterbodies unsuitable for human consumption, and inhospitable for marine fauna and flora.
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<font class="font-class-9"><center><b>Join us in our vision for the <br><br> Biomanufacturing of the Future.</b></center></font><br>
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Already, Citarum River in Indonesia is a clear example of this murky problem. The 30 million residents relying on the river as their only water source and livelihood are experiencing adverse skin conditions and increased exposure to infectious diseases, while the river has almost no aquatic life left. In some areas of the river, lead levels at more than 1,000 times the USEPA standard in drinking water have been found. River water rapidly changing colours from red, to green, yellow, and black due to high concentrations of dye is not an uncommon sight. However, Citarum River is not an isolated case. Similar sights can be seen in China’s Pearl River, Buriganga River in Bangladesh and Bagmati River in India. In Bangladesh, Dhaka, 719 factories and textile mills generate close to 200 metric tons of wastewater per ton of fabric per year. On the global scale, the textile dyeing and dyestuff production industry is the second most pollutive industry, coming in only after oil, and also uses the most water apart from agriculture.
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In recent years, some efforts have been put in place to revive natural dyes as a more sustainable alternative to synthetic dyes. However, this motion has not been gaining much traction, as traditional natural dye production faces many constraints. Natural dyes produced traditionally often have inconsistent quality, varying from batch to batch of plants used. Furthermore, it is land and labour intensive, and competes with food production for land use.
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<h2 id='Overview'>The Problem Statement</h2>
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    <p style="margin: 0 5%"> Our team believes that synthetic dyes are the unsustainable solution, and have taken the synthetic biology approach to natural dye bioproduction in the hopes of making natural dyes a stronger, better substitute to synthetic dyes. What this means is our dyes have to be non-toxic, have reduced use of chemicals in its production, environmentally friendly and appealing to fashion designers and consumers. 
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Latest revision as of 01:47, 14 December 2018

CONNECT WITH US

Biomanufacturing opens up possibilities for sustainable
production of useful materials.

We, Team NUSGEM, created Coup Dy’état,
an environmentally-friendly platform to advance biomanufacturing.

Discover our project
Current biomanufacturing platforms are
chemical-intensive and expensive.

Coup Dy’état is not only environmentally-friendly, but also potentially cheaper.

Find out how we designed our platform
Integrating findings from our human practices has helped us make sure that our platform is relevant and useful to biomanufacturers.

Find out who we spoke to


To prove that our platform works, we decided to produce flavonoids to be used as dyes.

This is because the textile dyeing and dyestuff production industry is the second most pollutive industry in the world.

Our biomanufacturing platform can
help.

See our results
No system would work without its support.

Find out how we modelled our complete system and how we built our hardware system to support our platform.
Our team of 13 is made up intensely motivated students across all disciplines.

Find out who we are
Our Achievements

Here's a lovely photo of us, flush with post-Jamboree endorphins! Click to read more on our university's website.

We are thrilled to announce that Coup Dy’état has won a Gold Medal and obtained 3 nominations for special awards: Best Manufacturing Project, Best Hardware, and the Safety Commendation. Thank you for being with us on our iGEM journey!



Join us in our vision for the

Biomanufacturing of the Future.



HEAR OUR STORY

We’re using synthetic biology to change the way the world thinks and functions.

We’re a little disruptive, dangerous and maybe a tad too bold. But the world needs some shaking up every now and then, and we think we’re just the right people for that.

Together we’re going to engineer biology and create something awesome.

CONTACT US

nusigem@gmail.com

21 Lower Kent Ridge Rd, Singapore 119077