Difference between revisions of "Team:BioIQS-Barcelona/Results"

 
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<h1> Results </h1>
 
  
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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                        <h1 class="mb-5">Project | Results</h1>
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                        <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Results#first-steps" class="btn btn-outline btn-xl js-scroll-trigger">Have a look!</a>
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                    <h2 class="section-heading orange" id="cuttheword">Achievements of our project</h2>
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                        <h1 class="book orange"><i>1.</i></h1>
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                                    <h4 class="book orange-medium">Computationally <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Standardization">demonstrated</a> that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes, or a specific HLA-DQ2- or HLA-DQ8-primer can be designed, reducing the cost of the sensor.</h4>
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                                </div>
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                        <h1 class="book orange"><i>2.</i></h1>
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                                    <h4 class="book orange-medium">Successfully <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Basic_Part">designed</a> a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all HLA-DQ existing variants possible using only one protocol.</h4>
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                        <h1 class="book orange"><i>3.</i></h1>
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                                <div class="col-md-12">
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                                    <h4 class="book orange-medium">Thoroughly designed and characterized multiple <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Composite_Part">composite parts</a> to ensure the correct expression of the human HLA-DQ presenting protein in a bacterial or yeast host. This would enable us to determine the best way to produce the elements needed for the sensor.</h4>
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                        <h1 class="book orange"><i>4.</i></h1>
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                                    <h4 class="book orange-medium">Correctly cloned one of the constructs into the desired expression vector and prepared it for further expression experiments. Cloning is a tedious work and not always results as one would expect so this is an important achievement!</h4>
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                        <h1 class="book orange"><i>5.</i></h1>
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                                    <h4 class="book orange-medium">Carefully built a <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Model">theoretical model</a> to illustrate the essential detection mechanism of the sensor. Modeling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.</h4>
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<p> Describe what your results mean </p>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what your data means, beyond all the data you have acquired during the project. </li>
 
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<li> A list of linked bullet points of the successful results during the project. </li>
 
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<li> A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort. </li>
 
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Latest revision as of 18:56, 17 December 2018

BIO IQS

Project | Results

Have a look!

Achievements of our project

1.

Computationally demonstrated that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes, or a specific HLA-DQ2- or HLA-DQ8-primer can be designed, reducing the cost of the sensor.

2.

Successfully designed a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all HLA-DQ existing variants possible using only one protocol.

3.

Thoroughly designed and characterized multiple composite parts to ensure the correct expression of the human HLA-DQ presenting protein in a bacterial or yeast host. This would enable us to determine the best way to produce the elements needed for the sensor.

4.

Correctly cloned one of the constructs into the desired expression vector and prepared it for further expression experiments. Cloning is a tedious work and not always results as one would expect so this is an important achievement!

5.

Carefully built a theoretical model to illustrate the essential detection mechanism of the sensor. Modeling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.