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</div> | </div> | ||
<p>Our first day in the laboratory</p></div> | <p>Our first day in the laboratory</p></div> | ||
− | <div class="collapsible-body"><span> Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics. | + | <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics. |
− | <br>Preparation of LA- medium <br> | + | <br> <b>Preparation of LA- medium </b> <br> |
LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C. | LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C. | ||
Revision as of 12:12, 9 July 2018
Notebook
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Week25
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Jun18
Test
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Jun19
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Jun20
Our first day in the laboratory
Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
Preparation of LA- medium
LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.- 1L Distilled water
- 5g Yeast extract
- 10g Tryptone
- 5g NaCl
- 15g Agar
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Jun21
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Jun22
Isolation and verification of pgRNA and pdCas9
Test
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InterLabs Study
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