Difference between revisions of "Team:NTNU Trondheim/Notebook"

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               </div>
 
               </div>
 
             </div>
 
             </div>
−
             <p>Our first day in the laboratory</p></div>
+
             <p>Our first day in the laboratory!</p></div>
 
             <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.  
 
             <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.  
  
 
<br> <b> Preparation of LA- medium </b> <br>
 
<br> <b> Preparation of LA- medium </b> <br>
−
LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.
+
LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.
  
 
<ul>  
 
<ul>  
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<ul>
 
<ul>
  
−
<li>0,5L    LA-medium </li>
+
<li>0.5L    LA-medium </li>
  
−
<li>0,5mL AMP (50mg/mL)</li>
+
<li>0.5mL AMP (50mg/mL)</li>
 
</ul>
 
</ul>
 
<br>
 
<br>
 
LA-medium with CM:
 
LA-medium with CM:
 
<ul>
 
<ul>
−
<li>0,5L    LA-medium</li>
+
<li>0.5L    LA-medium</li>
  
−
<li>0,5mL CM (35mg/mL)</li>
+
<li>0.5mL CM (35mg/mL)</li>
 
</ul>
 
</ul>
  

Revision as of 12:28, 9 July 2018

Notebook


  • Week
    25

    Note title

    • Jun
      18

      Test

      Test.
    • Jun
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      20

      Our first day in the laboratory!

      Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
      Preparation of LA- medium
      LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.
      • 1L Distilled water
      • 5g Yeast extract
      • 10g Tryptone
      • 5g NaCl
      • 15g Agar

      Agar plates with antibiotics
      Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
      LA- medium with AMP:
      • 0.5L LA-medium
      • 0.5mL AMP (50mg/mL)

      LA-medium with CM:
      • 0.5L LA-medium
      • 0.5mL CM (35mg/mL)
      The LA-media with antibiotic were poured on petri dishes.
    • Jun
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      22

      Isolation and verification of pgRNA and pdCas9

      Test
  • Week
    26

    Note title

    • Jun
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      27

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      28

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      29

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    27

    Note title

    • Jul
      1

      Note title

    • Jul
      2

      InterLabs Study

      Calibration 1 and 2 for the InterLabs Study.
    • Jul
      3

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      4

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      5

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    28

    Note title

    • Jul
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      10

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      11

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      12

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      13

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    29

    Note title

    • Jul
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      17

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      18

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      20

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    30

    Note title

    • Jul
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      24

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      27

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    31

    Note title

    • Jul
      30

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      31

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      1

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      2

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      3

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    32

    Note title

    • Aug
      6

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      7

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      8

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      10

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    33

    Note title

    • Aug
      13

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      14

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      15

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      17

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    34

    Note title

    • Aug
      20

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      22

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      24

      Note title

      Lorem ipsum dolor sit amet.