Revision as of 12:56, 9 July 2018

Notebook

Week

25

Note title
- Jun
  
  18
  
  Test
  
  Test.
- Jun
  
  19
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  20
  
  Our first day in the laboratory!
  Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
  
  Preparation of LA- medium
  LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.
  
  1L Distilled water
  
  5g Yeast extract
  
  10g Tryptone
  
  5g NaCl
  
  15g Agar
  
  Agar plates with antibiotics
  Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
  LA- medium with AMP:
  
  0.5L LA-medium
  
  0.5mL AMP (50mg/mL)
  
  LA-medium with CM:
  
  0.5L LA-medium
  
  0.5mL CM (35mg/mL)
  
  The LA-media with antibiotic were poured on petri dishes.
- Jun
  
  21
  
  Inoculation of E.coli from agar plates to LB-medium
  
  Goal: Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics.
  
  Procedure
  One colony of pgRNA- and pdCas9- bacteria grown overnight were picked and inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm.
- Jun
  
  22
  
  Isolation and verification of pgRNA and pdCas9
  
  Test
Week

26

Note title
- Jun
  
  25
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  26
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  27
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  28
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jun
  
  29
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

27

Note title
- Jul
  
  1
  
  Note title
- Jul
  
  2
  
  InterLabs Study
  
  Calibration 1 and 2 for the InterLabs Study.
- Jul
  
  3
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  4
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  5
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

28

Note title
- Jul
  
  9
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  10
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  11
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  12
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  13
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

29

Note title
- Jul
  
  16
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  17
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  18
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  19
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  20
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

30

Note title
- Jul
  
  23
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  24
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  25
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  26
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  27
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

31

Note title
- Jul
  
  30
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Jul
  
  31
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  1
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  2
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  3
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

32

Note title
- Aug
  
  6
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  7
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  8
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  9
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  10
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

33

Note title
- Aug
  
  13
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  14
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  15
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  16
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  17
  
  Note title
  
  Lorem ipsum dolor sit amet.
Week

34

Note title
- Aug
  
  20
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  21
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  22
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  23
  
  Note title
  
  Lorem ipsum dolor sit amet.
- Aug
  
  24
  
  Note title
  
  Lorem ipsum dolor sit amet.

@@ Line 92: / Line 92: @@
               </div>
               <p>Our first day in the laboratory!</p></div>
-              <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
+              <div class="collapsible-body"><span> <b>Goal:</b> Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.<br><br>
-<br> <b> Preparation of LA- medium </b> <br>
+<b> Preparation of LA- medium </b> <br>
 LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.
@@ Line 150: / Line 150: @@
 <b>Goal:</b> Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics. <br> <br>
 <b>Procedure</b> <br>
-One colony of pgRNA- and pdCas9- bacteria were inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm. <br>
+One colony of pgRNA- and pdCas9- bacteria grown overnight were picked and inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm. <br>
 </span></div>
             </li>

Difference between revisions of "Team:NTNU Trondheim/Notebook"

Revision as of 12:56, 9 July 2018

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