Difference between revisions of "Team:BCU"

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{{BCU}}
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−
<h1> Nicotine degradation</h1>
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    <div id='globalWrapper'>
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<p>BCU|CHINA </p>
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                <div class="logo_2018">
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                    <a href="https://2018.igem.org">
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−
</div>  
+
        <h1 id="firstHeading" class="firstHeading">
 +
            <span dir="auto">Team:ASIJ Tokyo</span>
 +
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 +
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                <div id="bodyContent">
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            <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p>
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<div class="column full_size" >
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/* Reset the wiki style by overriding with a reset stylesheet (written by Florian Albrecht)
 +
  the relevant wiki css rules are reset by using rules with higher priority */
  
−
<h3>Before you start</h3>
 
−
<p> Please read the following pages:</p>
 
−
<ul>
 
−
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
 
−
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
−
</ul>
 
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<div class="column full_size" >
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#liver {
−
<h3> wiki的样式 </h3>
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    position: relative;
−
<p>您可以按自己喜欢的方式对页面进行样式化，也可以简单地保持原样。您可以使用您的项目信息轻松地保持这些默认wiki页面的样式和编辑内容，并完全满足对项目文档的要求</p>
+
    left: 50px;
−
<p>虽然您可能无法获得最佳的Wiki，但您的团队仍然有资格获得所有其他奖项。这个默认的wiki满足了需求，它提高了访问者的可浏览性和易用性，并且您不应该认为有必要在提供的内容之外进行样式化。</p>
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−
<h3> Uploading pictures and files </h3>
+
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−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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−
<h3> Wiki template information </h3>
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−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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−
<h3> Editing your wiki </h3>
+
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−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
   
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
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EDIT PAGE
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−
<div class="column two_thirds_size" >
+
<style>
−
<h3>Tips</h3>
+
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
−
<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>
+
−
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+
  
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        <p>A1-AT<br>DE<span id="red">LIVER</span>Y </p>
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    <h1> Using Stem Cell and CRISPR Technology to <br> Combat Alpha-1 Antitrypsin Deficiency </h1>
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    <div id="abstract">
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          <h3> ABSTRACT </h3>
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          <p> Alpha-1 Antitrypsin deficiency is a common genetic disorder -- the defective gene for which is carried by 1 in 25 people -- which arises from a single base pair mutation in the SERPINA1 gene, resulting in the production of a form of antitrypsin prone to polymerization. The mutated antitrypsin then builds up in liver cells and is unable to inhibit proteases in the lungs, leading to damage in both. Using CRISPR-Cas9 technology, we aimed to fix the error in SERPINA1 so that proper antitrypsin can be produced. We will show proof of concept in E. Coli cells using osmy secretion tags and GFP as a reporter. We hope to design a liver organ bud using IPS cell technology to deliver function A1AT through collaboration with Dr. Kagimoto of Healios Japan KK. </p>
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<h3>Inspiration</h3>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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Team:ASIJ Tokyo

HOME TEAM PROJECT PARTS HUMAN PRACTICES SAFETY

A1-AT
DELIVERY

Using Stem Cell and CRISPR Technology to
Combat Alpha-1 Antitrypsin Deficiency

ABSTRACT

Alpha-1 Antitrypsin deficiency is a common genetic disorder -- the defective gene for which is carried by 1 in 25 people -- which arises from a single base pair mutation in the SERPINA1 gene, resulting in the production of a form of antitrypsin prone to polymerization. The mutated antitrypsin then builds up in liver cells and is unable to inhibit proteases in the lungs, leading to damage in both. Using CRISPR-Cas9 technology, we aimed to fix the error in SERPINA1 so that proper antitrypsin can be produced. We will show proof of concept in E. Coli cells using osmy secretion tags and GFP as a reporter. We hope to design a liver organ bud using IPS cell technology to deliver function A1AT through collaboration with Dr. Kagimoto of Healios Japan KK.