Difference between revisions of "Team:BCU"

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                        <h1 class="animated fadeInDown">BIT-China</h1>
 
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                    <h1 class="arrow">About us</h1>
 
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                    <p>This year, we will focus on how to identify the antioxidant capacity of antioxidants.. Antioxidants has
 
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                        been widely used in foods, Health products and cosmetic. But is there any measure way to know which
 
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                        antioxidant is better?</p>
 
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                    <h1 class="arrow">Measure ways before us</h1>
 
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                    <p>1. Most of the current detection methods for antioxidant properties are based on chemical assays, which
 
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                        has limited value for us since the redox reaction in vivo is quite different from that in vitro.
 
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                        <br> 2. Others also consider to transfer redox reactions to the intracellular environment by adding H2O2
 
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                        and different antioxidants in order, through observing whether the yeast died or not, they could
 
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                        know which antioxidant would work better. However, people still looking forward a more gentle way
 
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                        of representation.
 
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                    <h1 class="arrow">What we are trying to do</h1>
 
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                    <p>We are going to modified yeast to identify the antioxidant capacity of antioxidants. Single-cell eukaryotic
 
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                        yeasts have a similar apoptotic process as animal cells, and due to S. cerevisiae have advantages,
 
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                        Saccharomyces cerevisiae is an important model organism for studying apoptosis.</p>
 
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                    <h1 class="arrow">how to achieve that</h1>
 
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                    <p>To achieve that goal, our project will be separate into 4 parts:
 
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                        <br> 1. Regulate
 
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                        <br> Firstly, we will over express gene ndi1 (which may express ubiquinone oxidoreductase, transfers
 
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                        electrons from NADH to ubiquinone in the respiratory chain. It has been proved that over express
 
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                        ndi1 could accumulate ROS. [1]) to accumulate ROS (Reactive oxygen species). Besides, through knocking
 
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                        out gene yca1 (also known as MCA1, may expresses Ca(2+)-dependent cysteine protease MCA1. MCA1 could
 
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                        regulate apoptosis upon H2O2 treatment. Once knock it out, yeast may survive from high oxidative
 
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                        stress. [2]) we will improve the tolerance of ROS, which means our yeast will stay alive during the
 
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                        whole process of detection.
 
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                        <br> 2. Feedback
 
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                        <br> To ensure our yeast won’t die from large accumulation of ROS, we need to promptly turn off the
 
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                        expression of ROS. At the same time, this will also meet the detection range of ro-GFP.
 
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                        <br> 3. Input
 
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                        <br> after that, we will add different antioxidants into the medium, we will standardize the externally
 
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                        added antioxidants to make the results more feasible.
 
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                        <br> 4. Output
 
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                        <br> We will express orp-ro-GFP in our yeast to sense the ROS remaining, (it's a kind of Fusion protein,
 
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                        which will become oxidized and turn into orp-ox-GFP once react with ROS, and when the amount of ROS
 
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                        drop, it will back to orp-ro-GFP. Its two different states correspond to different wavelengths of
 
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                        excitation light, which has been described and used in
 
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                        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2296000">Cornell University 2017</a> ) to know the residual amount of yeast endogenous ROS in different states,
 
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                        so that we can compare the performance of different antioxidants, and conclude which antioxidant
 
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                        is better.
 
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                    <h1 class="arrow">CONTACT US</h1>
 
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                            <span>Twitter</span>
 
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                            <a href="https://mobile.twitter.com/BIT_China2018">BIT_China2018</a>
 
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                            <span>Facebook</span>
 
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                            <span>Email</span>
 
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                            <a href="mailto:bit_china@163.com">bit_china@163.com</a>
 
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                            <span>Ins</span>
 
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                            <a href="https://www.instagram.com/bit_china/">bit_china</a>
 
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Revision as of 13:58, 11 July 2018

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