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| − | | + | <style type="text/css"> |
| − | <style> | + | |
| | <!-- | | <!-- |
| − | #contentSub,
| + | body { |
| − | #footer-box,
| + | background-image: url(/wiki/images/e/ee/T--BCU--background.jpg); |
| − | #catlinks,
| + | background-repeat: no-repeat; |
| − | #search-controls,
| + | margin-left: 0px; |
| − | #p-logo,
| + | margin-top: 0px; |
| − | .printfooter,
| + | margin-right: 0px; |
| − | .firstHeading,
| + | margin-bottom: 0px; |
| − | .visualClear,
| + | } |
| − | #top_title {
| + | .title { |
| − | display: none;
| + | height: 200px; |
| − | }
| + | width: 960px; |
| − | | + | margin-right: auto; |
| − | /*-- hides default wiki settings --*/
| + | margin-left: auto; |
| − | | + | } |
| − | #globalWrapper,
| + | .nav { |
| − | #content {
| + | float: right; |
| − | width: 100%;
| + | height: 100px; |
| − | height: 100%;
| + | width: 750px; |
| − | margin: 0px;
| + | margin-top: 30px; |
| − | padding: 0px;
| + | padding-top: 30px; |
| − | }
| + | font-family: "Times New Roman", Times, serif; |
| − | | + | font-size: 18px; |
| − | #HQ_page .text-center {
| + | color: #FFFFFF; |
| − | text-align: center;
| + | font-weight: 400; |
| − | }
| + | letter-spacing: normal; |
| − | | + | } |
| − | #contact p {
| + | |
| − | text-align: center;
| + | |
| − | }
| + | |
| − | | + | |
| − | #intro p {
| + | |
| − | margin: 50px 0 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #measure p {
| + | |
| − | margin: 50px 0 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #approach p {
| + | |
| − | margin: 50px 0 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #target p {
| + | |
| − | margin: 50px 0 0;
| + | |
| − | }
| + | |
| | --> | | --> |
| | </style> | | </style> |
| − | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/temp-bootstrap?action=raw&ctype=text/css" /> | + | <script type="text/JavaScript"> |
| − | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/temp-font-awesome?action=raw&ctype=text/css" /> | + | <!-- |
| − | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/temp-style?action=raw&ctype=text/css" />
| + | function MM_preloadImages() { //v3.0 |
| − | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/temp-queries?action=raw&ctype=text/css" />
| + | var d=document; if(d.images){ if(!d.MM_p) d.MM_p=new Array(); |
| − | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/temp-animate?action=raw&ctype=text/css" />
| + | var i,j=d.MM_p.length,a=MM_preloadImages.arguments; for(i=0; i<a.length; i++) |
| | + | if (a[i].indexOf("#")!=0){ d.MM_p[j]=new Image; d.MM_p[j++].src=a[i];}} |
| | + | } |
| | | | |
| | + | function MM_findObj(n, d) { //v4.01 |
| | + | var p,i,x; if(!d) d=document; if((p=n.indexOf("?"))>0&&parent.frames.length) { |
| | + | d=parent.frames[n.substring(p+1)].document; n=n.substring(0,p);} |
| | + | if(!(x=d[n])&&d.all) x=d.all[n]; for (i=0;!x&&i<d.forms.length;i++) x=d.forms[i][n]; |
| | + | for(i=0;!x&&d.layers&&i<d.layers.length;i++) x=MM_findObj(n,d.layers[i].document); |
| | + | if(!x && d.getElementById) x=d.getElementById(n); return x; |
| | + | } |
| | | | |
| − | <body id="top">
| + | function MM_nbGroup(event, grpName) { //v6.0 |
| − | | + | var i,img,nbArr,args=MM_nbGroup.arguments; |
| − | <header id="home"> | + | if (event == "init" && args.length > 2) { |
| − |
| + | if ((img = MM_findObj(args[2])) != null && !img.MM_init) { |
| − | | + | img.MM_init = true; img.MM_up = args[3]; img.MM_dn = img.src; |
| − | <section class="hero" id="hero">
| + | if ((nbArr = document[grpName]) == null) nbArr = document[grpName] = new Array(); |
| − | <div class="container">
| + | nbArr[nbArr.length] = img; |
| − | <div class="row">
| + | for (i=4; i < args.length-1; i+=2) if ((img = MM_findObj(args[i])) != null) { |
| − | <div class="col-md-8 col-md-offset-2 text-center inner">
| + | if (!img.MM_up) img.MM_up = img.src; |
| − | <h1 class="animated fadeInDown">BIT-China</h1>
| + | img.src = img.MM_dn = args[i+1]; |
| − | </div>
| + | nbArr[nbArr.length] = img; |
| − | </div>
| + | } } |
| − | <div class="row">
| + | } else if (event == "over") { |
| − | <div class="col-md-6 col-md-offset-3 text-center">
| + | document.MM_nbOver = nbArr = new Array(); |
| − | <img src="https://static.igem.org/mediawiki/2018/d/da/T--BIT-China--iGEM2018_logo.png" height="100" width="100" />
| + | for (i=1; i < args.length-1; i+=3) if ((img = MM_findObj(args[i])) != null) { |
| − | </div>
| + | if (!img.MM_up) img.MM_up = img.src; |
| − | </div>
| + | img.src = (img.MM_dn && args[i+2]) ? args[i+2] : ((args[i+1])? args[i+1] : img.MM_up); |
| − | </div>
| + | nbArr[nbArr.length] = img; |
| − | </section>
| + | } |
| − | | + | } else if (event == "out" ) { |
| − | </header> | + | for (i=0; i < document.MM_nbOver.length; i++) { |
| − | | + | img = document.MM_nbOver[i]; img.src = (img.MM_dn) ? img.MM_dn : img.MM_up; } |
| − | <section class="intro text-center section-padding" id="intro">
| + | } else if (event == "down") { |
| − | <div class="container">
| + | nbArr = document[grpName]; |
| − | <div class="row">
| + | if (nbArr) |
| − | <div class="col-md-8 col-md-offset-2">
| + | for (i=0; i < nbArr.length; i++) { img=nbArr[i]; img.src = img.MM_up; img.MM_dn = 0; } |
| − | <h1 class="arrow">About us</h1>
| + | document[grpName] = nbArr = new Array(); |
| − | <p>This year, we will focus on how to identify the antioxidant capacity of antioxidants.. Antioxidants has
| + | for (i=2; i < args.length-1; i+=2) if ((img = MM_findObj(args[i])) != null) { |
| − | been widely used in foods, Health products and cosmetic. But is there any measure way to know which
| + | if (!img.MM_up) img.MM_up = img.src; |
| − | antioxidant is better?</p>
| + | img.src = img.MM_dn = (args[i+1])? args[i+1] : img.MM_up; |
| − | </div>
| + | nbArr[nbArr.length] = img; |
| − | </div>
| + | } } |
| − | </div>
| + | } |
| − | </section>
| + | //--> |
| | + | </script> |
| | | | |
| − | <section class="intro text-center section-padding" id="measure">
| + | </head> |
| − | <div class="container">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-md-8 col-md-offset-2">
| + | |
| − | <h1 class="arrow">Measure ways before us</h1>
| + | |
| − | <p>1. Most of the current detection methods for antioxidant properties are based on chemical assays, which
| + | |
| − | has limited value for us since the redox reaction in vivo is quite different from that in vitro.
| + | |
| − | <br> 2. Others also consider to transfer redox reactions to the intracellular environment by adding H2O2
| + | |
| − | and different antioxidants in order, through observing whether the yeast died or not, they could
| + | |
| − | know which antioxidant would work better. However, people still looking forward a more gentle way
| + | |
| − | of representation.
| + | |
| − | <br>
| + | |
| − | </p>
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </section>
| + | |
| | | | |
| − | <section class="intro text-center section-padding" id="approach">
| + | <body onload="MM_preloadImages('Home')"> |
| − | <div class="container">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-md-8 col-md-offset-2">
| + | |
| − | <h1 class="arrow">What we are trying to do</h1>
| + | |
| − | <p>We are going to modified yeast to identify the antioxidant capacity of antioxidants. Single-cell eukaryotic
| + | |
| − | yeasts have a similar apoptotic process as animal cells, and due to S. cerevisiae have advantages,
| + | |
| − | Saccharomyces cerevisiae is an important model organism for studying apoptosis.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </section>
| + | |
| | | | |
| − | <section class="intro text-center section-padding" id="target">
| |
| − | <div class="container">
| |
| − | <div class="row">
| |
| − | <div class="col-md-8 col-md-offset-2">
| |
| − | <h1 class="arrow">how to achieve that</h1>
| |
| − | <p>To achieve that goal, our project will be separate into 4 parts:
| |
| − | <br> 1. Regulate
| |
| − | <br> Firstly, we will over express gene ndi1 (which may express ubiquinone oxidoreductase, transfers
| |
| − | electrons from NADH to ubiquinone in the respiratory chain. It has been proved that over express
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| − | ndi1 could accumulate ROS. [1]) to accumulate ROS (Reactive oxygen species). Besides, through knocking
| |
| − | out gene yca1 (also known as MCA1, may expresses Ca(2+)-dependent cysteine protease MCA1. MCA1 could
| |
| − | regulate apoptosis upon H2O2 treatment. Once knock it out, yeast may survive from high oxidative
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| − | stress. [2]) we will improve the tolerance of ROS, which means our yeast will stay alive during the
| |
| − | whole process of detection.
| |
| − | <br> 2. Feedback
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| − | <br> To ensure our yeast won’t die from large accumulation of ROS, we need to promptly turn off the
| |
| − | expression of ROS. At the same time, this will also meet the detection range of ro-GFP.
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| − | <br> 3. Input
| |
| − | <br> after that, we will add different antioxidants into the medium, we will standardize the externally
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| − | added antioxidants to make the results more feasible.
| |
| − | <br> 4. Output
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| − | <br> We will express orp-ro-GFP in our yeast to sense the ROS remaining, (it's a kind of Fusion protein,
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| − | which will become oxidized and turn into orp-ox-GFP once react with ROS, and when the amount of ROS
| |
| − | drop, it will back to orp-ro-GFP. Its two different states correspond to different wavelengths of
| |
| − | excitation light, which has been described and used in
| |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2296000">Cornell University 2017</a> ) to know the residual amount of yeast endogenous ROS in different states,
| |
| − | so that we can compare the performance of different antioxidants, and conclude which antioxidant
| |
| − | is better.
| |
| − | <br>
| |
| − | </p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </section>
| |
| | | | |
| − | <section class="dark-bg text-center section-padding contact-wrap" id="contact">
| + | <div class="title"> |
| − | <div class="container">
| + | <table border="0" cellpadding="0" cellspacing="0"> |
| − | <div class="row">
| + | <tr> |
| − | <div class="col-md-12">
| + | <td><a href="javascript:;" target="_top" onclick="MM_nbGroup |
| − | <h1 class="arrow">CONTACT US</h1>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="row contact-details">
| + | ('down','group1','Home','Home',1)" onmouseover="MM_nbGroup('over','Home','Home','Home',1)" |
| − | <div class="col-md-3">
| + | |
| − | <div class="light-box box-hover">
| + | |
| − | <h2>
| + | |
| − | <i class="fa fa-twitter"></i>
| + | |
| − | <span>Twitter</span>
| + | |
| − | </h2>
| + | |
| − | <p>
| + | |
| − | <a href="https://mobile.twitter.com/BIT_China2018">BIT_China2018</a>
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="col-md-3">
| + | |
| − | <div class="light-box box-hover">
| + | |
| − | <h2>
| + | |
| − | <i class="fa fa-facebook"></i>
| + | |
| − | <span>Facebook</span>
| + | |
| − | </h2>
| + | |
| − | <p>
| + | |
| − | <a href="https://www.facebook.com/BIT_China2018-190488561659083/">BIT_China2018</a>
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="col-md-3">
| + | onmouseout="MM_nbGroup('out')"></a></td> |
| − | <div class="light-box box-hover">
| + | </tr> |
| − | <h2>
| + | </table> |
| − | <i class="fa fa-envelope"></i>
| + | < div class="nav"><a href=" https: //2018. igem.org/Team:BCU"> Home</a> | Team | Project | |
| − | <span>Email</span>
| + | |
| − | </h2>
| + | |
| − | <p>
| + | |
| − | <a href=" mailto: bit_china@163. com"> bit_china@163.com</a> | + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="col-md-3">
| + | Parts | Safety | </div> |
| − | <div class="light-box box-hover">
| + | </div> |
| − | <h2>
| + | |
| − | <i class="fa fa-instagram"></i>
| + | |
| − | <span>Ins</span>
| + | |
| − | </h2>
| + | |
| − | <p>
| + | |
| − | <a href="https://www.instagram.com/bit_china/">bit_china</a>
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </section>
| + | |
| | | | |
| | | | |
| − | <!-- jQuery (necessary for Bootstrap's JavaScript plugins) -->
| |
| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-jquery?action=raw&ctype=text/javascript"></script>
| |
| − | <!-- Include all compiled plugins (below), or include individual files as needed -->
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| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-waypoints?action=raw&ctype=text/javascript"></script>
| |
| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-bootstrap?action=raw&ctype=text/javascript"></script>
| |
| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-scripts?action=raw&ctype=text/javascript"></script>
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| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-jquery_flexslider?action=raw&ctype=text/javascript"></script>
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| − | <script src="https://2018.igem.org/Template:BIT-China/js/temp-modernizr?action=raw&ctype=text/javascript"></script>
| |
| | | | |
| | </body> | | </body> |
