Difference between revisions of "Team:NTNU Trondheim/Notebook"

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             <p>Isolation and verification of pgRNA and pdCas9</p></div>
 
             <p>Isolation and verification of pgRNA and pdCas9</p></div>
 
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             <div class="collapsible-body"><span>
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Verified that the bacteria in our pure cultures had been correctly transformed with the pgRNA and the pdCas9. This was done by plasmid isolation (Miniprep), restriction cutting with appropriate enzymes and agarose gel electrophoresis (test digest).
 
</span></div>
 
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           </li>
 
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Revision as of 14:23, 12 July 2018

Notebook


  • Week
    25

    Getting started

    • Jun
      18

      No lab

      Test.
    • Jun
      19

      No lab

      Contacted some potential sponsors and continued our search for new ones. The project description was entered on our wiki page. We had a quick lab tour guided by our instructor Nikolay.
    • Jun
      20

      Our first day in the laboratory!

      We finally started working in the laboratory. We made LA-medium and plated bacteria carrying pgRNA and pdCas9 on agar plates with ampicillin and chloramphenicol, respectively. More details about the lab procedures throughout the project are given in our lab journal.
    • Jun
      21

      Inoculation of E.coli

      Pure cultures were made by picking and inoculating colonies grown overnight on agar plates with antibiotics as selection agents.
    • Jun
      22

      Isolation and verification of pgRNA and pdCas9

      Verified that the bacteria in our pure cultures had been correctly transformed with the pgRNA and the pdCas9. This was done by plasmid isolation (Miniprep), restriction cutting with appropriate enzymes and agarose gel electrophoresis (test digest).
  • Week
    26

    Note title

    • Jun
      25

      Note title

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    • Jun
      26

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    • Jun
      27

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    • Jun
      28

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    • Jun
      29

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  • Week
    27

    Note title

    • Jul
      1

      Note title

    • Jul
      2

      InterLabs Study

      Calibration 1 and 2 for the InterLabs Study.
    • Jul
      3

      Note title

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    • Jul
      4

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    • Jul
      5

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  • Week
    28

    Note title

    • Jul
      9

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    • Jul
      10

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    • Jul
      11

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    • Jul
      12

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    • Jul
      13

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  • Week
    29

    Note title

    • Jul
      16

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    • Jul
      17

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    • Jul
      18

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    • Jul
      19

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    • Jul
      20

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      Lorem ipsum dolor sit amet.
  • Week
    30

    Note title

    • Jul
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      24

      Note title

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    • Jul
      25

      Note title

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    • Jul
      26

      Note title

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    • Jul
      27

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    31

    Note title

    • Jul
      30

      Note title

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    • Jul
      31

      Note title

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    • Aug
      1

      Note title

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    • Aug
      2

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    • Aug
      3

      Note title

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  • Week
    32

    Note title

    • Aug
      6

      Note title

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    • Aug
      7

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    • Aug
      8

      Note title

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    • Aug
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      10

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    33

    Note title

    • Aug
      13

      Note title

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    • Aug
      14

      Note title

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    • Aug
      15

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      17

      Note title

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  • Week
    34

    Note title

    • Aug
      20

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      22

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      23

      Note title

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    • Aug
      24

      Note title

      Lorem ipsum dolor sit amet.