|
|
| Line 171: |
Line 171: |
| | for(var i=0;i<sfEles.length; i++){ | | for(var i=0;i<sfEles.length; i++){ |
| | sfEles[i].onmouseover=function(){ | | sfEles[i].onmouseover=function(){ |
| − | this.className +=(this.className.length>0?"":"")+"listshow"; | + | this.className +=(this. |
| − | }
| + | |
| − | sfEles[i].onmouseout=function(){
| + | |
| − | this.className =this.className.replace("listshow","");
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | window.onload= menuFix;
| + | |
| − | function MM_changeProp(objId,x,theProp,theValue) { //v9.0
| + | |
| − | var obj = null; with (document){ if (getElementById)
| + | |
| − | obj = getElementById(objId); }
| + | |
| − | if (obj){
| + | |
| − | if (theValue == true || theValue == false)
| + | |
| − | eval("obj.style."+theProp+"="+theValue);
| + | |
| − | else eval("obj.style."+theProp+"='"+theValue+"'");
| + | |
| − | }
| + | |
| − | }
| + | |
| − | function MM_effectAppearFade(targetElement, duration, from, to, toggle)
| + | |
| − | {
| + | |
| − | Spry.Effect.DoFade(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
| + | |
| − | }
| + | |
| − | function MM_effectBlind(targetElement, duration, from, to, toggle)
| + | |
| − | {
| + | |
| − | Spry.Effect.DoBlind(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
| + | |
| − | }
| + | |
| − | </script>
| + | |
| − | </head>
| + | |
| − | | + | |
| − | <body>
| + | |
| − | <div id="d">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" width="100%" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', true)" />
| + | |
| − | <div align="center">
| + | |
| − | <div id="HOME">
| + | |
| − | <div class="sub">
| + | |
| − | <ul><li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li></ul></div>
| + | |
| − | <div class="sub">
| + | |
| − | <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></ul></li></div>
| + | |
| − | <ul>
| + | |
| − | <li><a href="#definition">definition</a></li>
| + | |
| − | <li><a href="#method">method</a></li>
| + | |
| − | <li><a href="#choose">choose</a></li>
| + | |
| − | <li><a href="#principle">principle</a></li>
| + | |
| − | <li><a href="#whole">whole</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="container" align="center">
| + | |
| − | <div id="menu">
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China">HOME</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts">PARTS</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Improved_Parts">Improved</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">OTHERS</a>
| + | |
| − | <ul>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | <div class="header"><img src="https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png" width="100%" >
| + | |
| − | </div>
| + | |
| − | <div class="contain">
| + | |
| − | <div style="position:relative;"><img src="https://static.igem.org/mediawiki/2017/e/e6/T-Nanjing-China-WIKIbackground-1.png" width="1%" style="z-index:3;"/></div>
| + | |
| − | <div id="definition">
| + | |
| − | <div class="word">
| + | |
| − | <h2>The definition of nitrogen fixation</h2>
| + | |
| − | <div class="word-2">
| + | |
| − | <h3>•What is nitrogen fixation?</h3>
| + | |
| − | <p align="left"><font color="#FF9900"> Nitrogen → ammonia (NH<sub>3</sub>)</font> or other molecules available to living organisms. </p>
| + | |
| − | <div><img src="https://static.igem.org/mediawiki/2018/1/17/T--Nanjing-China--i-background-1.png" width="80%" /></div>
| + | |
| − | </div>
| + | |
| − | <div class="word-2">
| + | |
| − | <div><img src="https://static.igem.org/mediawiki/2018/2/2a/T--Nanjing-China--i-background-2.png" width="50%" /></div>
| + | |
| − | <h2>What is nitrogen fixation for?</h2>
| + | |
| − | <p align="left"> <font color="#339900">-global food supply <br/>
| + | |
| − | -reduce the use of chemical nitrogen fertilizers </font> </p>
| + | |
| − | </div>
| + | |
| − | <div class="word-1"><div class="word-background-block" style="width:100%;"> </div>
| + | |
| − | <h4>Nitrogen fixation is essential for life. </h4>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div style="position:relative;"><img src="https://static.igem.org/mediawiki/2017/e/e6/T-Nanjing-China-WIKIbackground-1.png" width="1%" style="z-index:3;"/></div> <div id="method">
| + | |
| − | <div class="word">
| + | |
| − | <h2>The methods of nitrogem fixation</h2>
| + | |
| − | <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-3.gif" width="80%" /></div>
| + | |
| − | <div class="word-2">
| + | |
| − | <h3>Nitrogen cycle</h3>
| + | |
| − | <p align="left"><font color="#FF6600">•Nitrogen fixation:</font><br/>
| + | |
| − | —N<sub>2 </sub>→ plants by bacteria<br/>
| + | |
| − | <font color="#FF6600">•Nitrification:</font><br/>
| + | |
| − | —ammonium → nitrite → nitrate<br/>
| + | |
| − | —Absorbed by plants<br/>
| + | |
| − | <font color="#FF6600">•Denitrification:</font><br/>
| + | |
| − | —Release N to atmosphere</p>
| + | |
| − | <div class="word-background-block" style="height:180px"></div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="word">
| + | |
| − | <div class="word-2">
| + | |
| − | <div class="word-background-block" style="height:80px; width:100%;"></div>
| + | |
| − | <h3>Haber-Bosch process </h3>
| + | |
| − | <p align="left"><font color="#FF6600">N2 + 3H<sub>2</sub> → 2NH<sub>3</sub></font>(ΔH° = −91.8 kJ)<br/>
| + | |
| − | <font color="#FF6600">High temperature<br/>
| + | |
| − | High pressure<br />
| + | |
| − | Need too much energy<br /></font><br/>
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-4.gif" width="80%" /></div>
| + | |
| − | </div>
| + | |
| − | <div class="word">
| + | |
| − | <div class="word-background-block"></div>
| + | |
| − | <h3>Biological nitrogen fixation</h3>
| + | |
| − | <div class="word-2" style="width:60%"><img src="https://static.igem.org/mediawiki/2018/2/20/T--Nanjing-China--i-background-5.png" width="90%" /></div>
| + | |
| − | <div class="word-2" style="width:40%" align="left">
| + | |
| − | <p><font color="#CC66FF">Mild reaction conditions</font></p>
| + | |
| − | <p><font color="#0033CC">Relatively inexpensive</font></p>
| + | |
| − | <p><font color="#996600">High efficiency </font></p>
| + | |
| − | <p><font color="#0099FF">Environmentally friendly</font></p>
| + | |
| − | <div class="word-background-block"></div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div style="position:relative;"><img src="https://static.igem.org/mediawiki/2017/e/e6/T-Nanjing-China-WIKIbackground-1.png" width="1%" style="z-index:3;"/></div>
| + | |
| − | <div id="choose">
| + | |
| − | <div class="word-background-block"></div>
| + | |
| − | <div class="word">
| + | |
| − | <h2>The choose of nitrogenase</h2>
| + | |
| − | <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--Nanjing-China--i-background-6.png" width="90%" />
| + | |
| − | <p>Molybdenum <br />(MoFe)-dependent</p></div>
| + | |
| − | <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Nanjing-China--i-background-7.png" width="90%" />
| + | |
| − | <p>Vanadium <br />(VFe)-dependent</p></div>
| + | |
| − | <div class="word-3" ><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Nanjing-China--i-background-8.png" width="85%" />
| + | |
| − | <p>Iron-only <br />(FeFe)-dependent</p></div>
| + | |
| − | </div>
| + | |
| − | <div class="word">
| + | |
| − | <div class="word-background-block"></div><h4>MoFe has been studied extensively</h4>
| + | |
| − | </div>
| + | |
| − | <div class="word">
| + | |
| − | <div class="word-background-block"></div><h3>Nitrogen Fixation Gene Cluster </h3>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Nanjing-China--i-background-9.png" width="85%" />
| + | |
| − | <h4>The minimal nif genes required for nitrogen fixation.</h4>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div style="position:relative;"><img src="https://static.igem.org/mediawiki/2017/e/e6/T-Nanjing-China-WIKIbackground-1.png" width="1%" style="z-index:3;"/></div> <div id="principle">
| + | |
| − | <div class="word">
| + | |
| − | <h2>General principle of biological nitrogen fixation </h2>
| + | |
| − | <table width="95%" border="1" cellspacing="1" cellpadding="1">
| + | |
| − | <tr>
| + | |
| − | <td rowspan="2"><font color="#0033CC">01 Hydrolysis of ATP</font></td>
| + | |
| − | <td width="65%"> </td>
| + | |
| − | <td rowspan="2"><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td><img src="https://static.igem.org/mediawiki/2018/2/22/T--Nanjing-China--i-background-10.png" width="100%"/><p align="center">Chemical energy (ATP)→Solar energy</p></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td><font color="#CCCC00">02 Electron transfer </font></td>
| + | |
| − | <td width="65%"> </td>
| + | |
| − | <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | </div>
| + | |
| − | <div class="word">
| + | |
| − | <div class="word-background-block"></div>
| + | |
| − | <table width="95%" border="1" cellspacing="1" cellpadding="1">
| + | |
| − | <tr>
| + | |
| − | <td rowspan="2"><font color="#0033CC">01 Receive light signals</font></td>
| + | |
| − | <td width="65%" > </td>
| + | |
| − | <td rowspan="2"><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td ><img src="https://static.igem.org/mediawiki/2018/e/e3/T--Nanjing-China--i-background-11.png" width="100%" top="20px"/></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td><font color="#CCCC00">02 Electron transfer </font></td>
| + | |
| − | <td width="65%" > </td>
| + | |
| − | <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div style="position:relative;"><img src="https://static.igem.org/mediawiki/2017/e/e6/T-Nanjing-China-WIKIbackground-1.png" width="1%" style="z-index:3;"/></div> <div id="whole">
| + | |
| − | <div class="word">
| + | |
| − | <h2>The characteristics of whole-cell</h2>
| + | |
| − | <p><font size="110%" color="#CC6633">The property and advantage of whole-cells</font></p>
| + | |
| − | <p>•Fast proliferation to enable large-scale application<br />
| + | |
| − | •Good portability to permit on-site monitoring<br />
| + | |
| − | •Inexpensive and easy to preserve<br />
| + | |
| − | • Intracellular cascade reactions to amplify signals<br/>
| + | |
| − | • Excellent specificity, sensitivity and stability</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="footer">
| + | |
| − | <div align="center">
| + | |
| − | <div class="f">
| + | |
| − | <div class="f-word"><p><strong><u>Address</u></strong></p>
| + | |
| − | <p>Life Science Department<br />
| + | |
| − | #163 Xianlin Blvd, Qixia District<br />
| + | |
| − | Nanjing University<br />
| + | |
| − | Nanjing, Jiangsu Province<br />
| + | |
| − | P.R. of China<br />
| + | |
| − | Zip: 210046
| + | |
| − | </p>
| + | |
| − | <p><strong><u>Email</u></strong></p>
| + | |
| − | <p>nanjing_china@163.com</p>
| + | |
| − | <p><strong><u>Social media</u></strong></p>
| + | |
| − | <p>Twitter button: <br/>
| + | |
| − | <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
| + | |
| − | </div>
| + | |
| − | <div class="f-img">
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="160" height="220" id="img1" /></div>
| + | |
| − | </div>
| + | |
| − | <div class="f-img">
| + | |
| − | <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png
| + | |
| − | " name="img2" width="160" height="220" id="img2" /></div>
| + | |
| − | </div>
| + | |
| − | <div class="f-img">
| + | |
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="160" height="160" id="img3" /></div>
| + | |
| − | </div>
| + | |
| − | <div class="f-img">
| + | |
| − | <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="160" height="160" id="img4" /></div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="f-b"><img src="https://static.igem.org/mediawiki/2017/9/91/T-Nanjing-China-foot.png" width="100%" /></div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </body>
| + | |
| − | </html>
| + | |
