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Revision as of 10:05, 18 July 2018
Background
- Biosynthesis plays a significant role in industrial production while enzymatic reaction rate is one of the key factors in an efficient production. and concentration of enzymes is a widely employed solution.
Scaffold and Network
- Here we engineered yeast cells to express enzymes for producing carotene, which would then be integrated into a protein scaffold based on the interaction between SUMO and SIM modules. The oligomerization of these modules could induce the proximity of enzymes, which enhances the speed of enzymatic reactions and the yield rate of products significantly.
Reference
- Kim S, Hahn J S. Synthetic scaffold based on a cohesin–dockerin interaction for improved production of 2,3-butanediol in Saccharomyces cerevisiae[J]. Journal of Biotechnology, 2014, 192 Pt A(Pt A):192.
- Li Y J, Stark J M, Chen D J, et al. Role of SUMO:SIM-mediated protein|[ndash]|protein interaction in non-homologous end joining[J]. Oncogene, 2015, 29(24):3509-18.
- Verwaal R, Wang J, Meijnen J P, et al. High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous[J]. Applied & Environmental Microbiology, 2007, 73(13):4342.