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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan">Home</a>
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| − | <li><a href="#">our team</a></li>
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| − | <div <div class="interlab">>
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| − | <p class="first"> Transform (homemade e-coli dh5α)</p>
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| − | <p class="description">1. Take competent cells out of -80°C and thaw on ice (approximately 20-30 mins). </p>
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| − | <p class="description">2.Remove agar plates (containing the appropriate antibiotic) from storage at 4°C and let warm up to room temperature and then (optional) incubate in 37°C incubator. </p>
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| − | <p class="description">3.Mix 5 μl of DNA into 50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times. </p>
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| − | <p class="description">4.Incubate the competent cell/DNA mixture on ice for 30 mins. </p>
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| − | <p class="description">5.Heat shock each transformation tube into a 42°C water bath for 90 secs. </p>
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| − | <p class="description">6.Put the tubes back on ice for 5 min. </p>
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| − | <p class="description">7.Add 50 μl LB to the bacteria and grow in 37°C shaking incubator for 180 min. </p>
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| − | <p class="description">8.Spin down </p>
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| − | <p class="description">9.Plate all of the transformation onto a 10 cm LB agar plate containing the appropriate antibiotic (Chloramphenicol). </p>
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| − | <p class="description">10.Incubate plates at 37°C overnight. </p>
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