|
|
| (2 intermediate revisions by one other user not shown) |
| Line 1: |
Line 1: |
| − | {{CCU_Taiwan}}
| |
| − | <html>
| |
| | | | |
| − | <head>
| |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
| |
| − | <link rel=stylesheet type="text/css" href="CCU.css">
| |
| − | <script>
| |
| − | </script>
| |
| − | </head>
| |
| − |
| |
| − | <body>
| |
| − | <div class="all">
| |
| − | <header>
| |
| − | <nav class="navbar navbar-default navbar-inverse navbar-fixed-top">
| |
| − | <div class="container">
| |
| − | <ul>
| |
| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan">Home</a>
| |
| − | <ul>
| |
| − | <li><a href="#">our team</a></li>
| |
| − | <li><a href="#">resourses</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li><a href="#">Project</a>
| |
| − | <ul>
| |
| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab">Interlab</a></li>
| |
| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li><a href="#">Result</a></li>
| |
| − | <li><a href="#">Human Practice</a></li>
| |
| − | <li><a href="#">Modeling</a></li>
| |
| − | <li><a href="#">Safety</a></li>
| |
| − | <li><a href="#">Medals</a></li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </nav>
| |
| − | </header>
| |
| − | <div <div class="interlab">>
| |
| − | <p class="first"> Transform (homemade E-coli DH5α)</p>
| |
| − | <br>
| |
| − | <p class="description">1. Take competent cells out of -80°C and thaw on ice (approximately 20-30 mins). </p>
| |
| − | <br>
| |
| − | <p class="description">2.Remove agar plates (containing the appropriate antibiotic) from storage at 4°C and let warm up to room temperature and then (optional) incubate in 37°C incubator. </p>
| |
| − | <br>
| |
| − | <p class="description">3.Mix 5 μl of DNA into 50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times. </p>
| |
| − | <br>
| |
| − | <p class="description">4.Incubate the competent cell/DNA mixture on ice for 30 mins. </p>
| |
| − | <br>
| |
| − | <p class="description">5.Heat shock each transformation tube into a 42°C water bath for 90 secs. </p>
| |
| − | <br>
| |
| − | <p class="description">6.Put the tubes back on ice for 5 min. </p>
| |
| − | <br>
| |
| − | <p class="description">7.Add 50 μl LB to the bacteria and grow in 37°C shaking incubator for 180 min. </p>
| |
| − | <br>
| |
| − | <p class="description">8.Spin down </p>
| |
| − | <br>
| |
| − | <p class="description">9.Plate all of the transformation onto a 10 cm LB agar plate containing the appropriate antibiotic (Chloramphenicol). </p>
| |
| − | <br>
| |
| − | <p class="description">10.Incubate plates at 37°C overnight. </p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </body>
| |
| − |
| |
| − | </html>
| |
