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| − | <meta name="viewport" content="width=device-width, initial-scale=1">
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| − | <link rel=stylesheet type="text/css" href="CCU.css">
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| − | <script>
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| − | <nav class="navbar navbar-default navbar-inverse navbar-fixed-top">
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| − | <div class="container">
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| − | <ul>
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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan">Home</a>
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| − | <ul>
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| − | <li><a href="#">Our team</a></li>
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| − | <li><a href="#">Resourses</a></li>
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| − | </ul>
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| − | </li>
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| − | <li><a href="#">Parts</a></li>
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| − | <li><a href="#">Modeling</a>
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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Overview</a></li>
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| − | <li><a href="#">Project</a>
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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab">Interlab</a></li>
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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/Project/Protocol">Protocol</a></li>
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| − | <li><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
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| − | </ul>
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| − | </li>
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| − | <li><a href="#">Result</a></li>
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| − | <li><a href="#">Human Practice</a></li>
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| − | <li><a href="#">Medals</a></li>
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| − | <div class="interlab">
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| − | <p class="first"> Transform (homemade E-coli DH5α)</p>
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| − | <br>
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| − | <p class="description">1. Take competent cells out of -80°C and thaw on ice (approximately 20-30 mins). </p>
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| − | <br>
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| − | <p class="description">2.Remove agar plates (containing the appropriate antibiotic) from storage at 4°C and let warm up to room temperature and then (optional) incubate in 37°C incubator. </p>
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| − | <br>
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| − | <p class="description">3.Mix 5 μl of DNA into 50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times. </p>
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| − | <br>
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| − | <p class="description">4.Incubate the competent cell/DNA mixture on ice for 30 mins. </p>
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| − | <br>
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| − | <p class="description">5.Heat shock each transformation tube into a 42°C water bath for 90 secs. </p>
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| − | <p class="description">6.Put the tubes back on ice for 5 min. </p>
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| − | <br>
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| − | <p class="description">7.Add 50 μl LB to the bacteria and grow in 37°C shaking incubator for 180 min. </p>
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| − | <br>
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| − | <p class="description">8.Spin down </p>
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| − | <br>
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| − | <p class="description">9.Plate all of the transformation onto a 10 cm LB agar plate containing the appropriate antibiotic (Chloramphenicol). </p>
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| − | <br>
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| − | <p class="description">10.Incubate plates at 37°C overnight. </p>
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