Line 60: | Line 60: | ||
<div class = "column full_size"> | <div class = "column full_size"> | ||
− | <p>Plasmid 1 uses an AraC and pBAD promoter to regulate expression of PETase and MHETase. The transcription factor made from AraC usually binds and represses the pBAD promoter, halting transcription of the plasmid. The inducer, | + | <p>Plasmid 1 uses an AraC and pBAD promoter to regulate the expression of PETase and MHETase. The transcription factor made from AraC usually binds and represses the pBAD promoter, halting transcription of the plasmid. The inducer, arabinose, can be added to the media, and this molecule binds to the AraC transcription factor on the DNA strand and changes its conformation so that transcription can occur [1]. The reaction scheme on the left explains a more complete mechanism of the transcription/translation of these proteins. The creation of AraC protein is related to a constitutive promoter which we assume enters the system as a constant rate, K. This method was also used by the UC Davis iGEM team in 2012. We assumed a fast-equilibrium hypothesis on the formation of the dimer and an essentially constant pool of arabinose in the environment. We can also streamline the binding of the two arabinose inducers to the AraC dimer into one reaction determined by the rate parameters k3+ and k3-. Since the amount of RNA polymerase does not change relative to these molecules, and since the frequent assumption used in literature is to group the transcription and translation rate into one overall rate of protein production, we simplify the system further. From these assumptions, we can simplify the system down into the system shown on the right.</p> |
<center> | <center> |
Revision as of 16:25, 31 July 2018