Difference between revisions of "Team:Tianjin/Description"

(Blanked the page)
Line 1: Line 1:
 +
<html>
 +
<head>
 +
<meta charset="utf-8">
 +
<meta http-equiv="X-UA-Compatible" content="IE=Edge,chrome=1"/>
 +
<meta content="width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0;" name="viewport">
 +
<title>Team:Tianjin - 2018.igem.org</title>
 +
    <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css">
 +
<script src="https://2016.igem.org/wiki/index.php?title=Team:Tianjin/js/material/jquery&action=raw&ctype=text/javascript"></script>
 +
<script src="https://2017.igem.org/Team:Tianjin/Resources/JS:leftbarbootstrap?action=raw&ctype=text/javascript"></script>
 +
<!-- delete.css  -->
 +
<style>
 +
#globalWrapper,#content{background-color:transparent;border:0;border-bottom:0;float:none;margin:0;padding:0;width:100%;list-style-image:none}#top_title{margin:0}#HQ_page th{background-color:transparent;padding:0;color:black;border:0;border-collapse:collapse;vertical-align:text-top;text-transform:uppercase;text-align:center}#HQ_page td{padding:0;border:0;vertical-align:text-top;text-align:center;line-height:35px}#HQ_page .jumbotron p{text-align:center;margin-bottom:15px;font-weight:200}#HQ_page p{text-align:center}body{margin-bottom:-10px}#top_title,#sideMenu{display:none}a:hover{text-decoration:none}.mw-content-ltr ul{padding-top:0;padding-right:0;margin:auto}body{margin-top:0}#wrapper{padding-left:0}#page-wrapper{width:100%;padding:0}.intro{background-size:cover;width:100%;position:relative}.huge{font-size:50px;line-height:normal}
 +
</style>
  
 +
<!-- base.css -->
 +
<style>
 +
body{background-color:#f7f5e6}.navbar-default{background-color:white;border:solid transparent;padding-top:16px}.navbar-nav>li{margin-right:5px;margin-left:5px}.navbar-brand{height:auto!important;padding:0!important;line-height:25px!important;*/}.navbar-toggle{margin-top:25px!important;border-color:rgba(1,1,1,0)!important}.navbar-toggle:hover,.navbar-toggle:focus{background-color:rgba(1,1,1,0)!important}.navbar-default .navbar-nav>.open>a{background-color:white!important;color:green!important}.dropdown-menu>li>a{background-color:white!important}.dropdown-menu>li>a:hover{background-color:white!important;color:green!important}.open{opacity:1!important;z-index:1998;background-color:white}#footer h4{color:white;font-size:25px!important;padding-top:10px;padding-bottom:10px}#footer p{color:grey;padding-top:10px;padding-bottom:10px}.footer-part{padding-bottom:15px;padding-top:15px}#footer a{color:grey;padding-bottom:10px!important;padding-top:10px!important}#footer a:hover,#footer a:focus{color:white;-moz-transition:all .3s;-ms-transition:all .3s;-o-transition:all .3s;-webkit-transition:all .3s;transition:all .3s}#footer{margin-top:200px}
 +
</style>
 +
 +
<!--  home1-0.css  -->
 +
 +
<style>
 +
#MainPic{position:relative;z-index:0;background-size:contain;background-repeat:no-repeat}#bannercontent{height:75%;box-sizing:border-box;margin:auto}#bannercontent img{background-size:cover;width:100%;height:auto}.mask{margin-top:20px;margin-bottom:20px;margin-left:auto;margin-right:auto}.mask img{width:200px;height:200px;cursor:pointer;transition:all .6s}.mask img:hover{transform:scale(1.1)}.texta{position:fixed;top:0;width:100%;height:100%;background-color:rgba(0,0,0,0.5);z-index:10000}.textChange{position:absolute;margin:auto;top:10%;bottom:10%;left:0;right:0;background:#FFF;border-radius:.4rem .4rem .4rem .4rem;text-align:center;box-shadow:0 0 20px rgba(0,0,0,0.2)}.textChange img{width:200px;height:200px;margin-top:20px;margin-bottom:20px}.textforchange{padding-bottom:50px;margin-left:auto;padding-top:50px;margin-right:auto;width:300px;border:1px solid rgba(0,0,0,0.5);box-shadow:2px 2px 3px #aaa}.textAnimation{-webkit-animation-name:textscale;-webkit-animation-duration:.5s;-webkit-animation-timing-function:linear;-webkit-animation-iteration-count:infinite;-webkit-animation-iteration-count:1}@keyframes textscale{0%{transform:scale(0,0)}50%{transform:scale(1.2,1.2)}100%{transform:scale(1,1)}}.vi{visibility:visible!important;display:block!important;width:20px;height:20px;top:0;position:absolute;right:0;background-repeat:no-repeat;background-size:100% 100%;-moz-background-size:100% 100%;-webkit-background-size:100% 100%;background-image:url(https://static.igem.org/mediawiki/2018/3/31/T--Tianjin--close.jpg)}
 +
</style>
 +
 +
<!--  text.css  -->
 +
<style>
 +
.titleBox{padding-top:200px}.textCon{padding-left:100px;padding-right:100px}.title{text-align:center;font-weight:400;font-family:'Quicksand',sans-serif;text-align:center;border-bottom:0}.title-big{}.title-normal{margin-bottom:25px;color:#5265f0}.title-small{;color:#222;}.partition{padding-top:130px}.text{margin-top:25px;margin-bottom:50px;font-weight:400!important;font-size:18px!important;line-height:1.5}.picture{margin-top:25px}.navbar-collapse{font-family:'Rounded Rough',sans-serif!important;font-size:20px!important}.text p{text-align:left !important;font-size:18px !important;}.title-normal p{font-size:2em!important;}.title-big p{font-size:3em !important;}.title-small p{font-size:1.8em !important}
 +
</style>
 +
 +
 +
<style>
 +
#top_title{display:none;}
 +
.igem_2018_team_menu{display:none !important;}
 +
</style>
 +
<script src="https://use.fontawesome.com/597ba5ca72.js"></script>
 +
<script>
 +
/*$('#top_title').remove();$("#globalWrapper").removeAttr("id");$("#content").removeAttr("id");$("#HQ_page").removeAttr("id");$('.mw-body').removeClass('mw-body');$('.igem_2018_team_mobile_bar').remove();
 +
$('#bodyContent').removeAttr("id");
 +
$('#mw-content-text').removeAttr("id");
 +
$('.mw-content-ltr').removeAttr("class");
 +
$('.igem_2018_team_menu displaying_menu').remove();
 +
$('.igem_2018_team_content').removeAttr("class");
 +
$('.igem_2018_team_column_wrapper').removeAttr("class");
 +
$('.igem_2018_team_menu').remove();*/
 +
</script>
 +
</head>
 +
 +
<body>
 +
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation" style="box-shadow: grey 0em -0.7em 2.1em 0.1em;padding-top:16px;">
 +
    <div class="container-fluid">
 +
        <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-main-collapse">
 +
            <i class="fa fa-bars"></i>
 +
        </button>
 +
    <div class="navbar-header">
 +
        <a class="navbar-brand" href="https://2018.igem.org/Team:Tianjin">
 +
            <img class="img-responsive" style="float: left;" src="https://static.igem.org/mediawiki/2018/4/45/T--Tianjin--logo.png" height="80" width="80">
 +
            <div class="navbar-header" style="float:right;padding-top: 25px;margin-right: 5px;">
 +
        <span style="color: black; font-size: 30px;font-weight: 550;padding-left: 20px;font-family: Cyborg,sans-serif !important"> iGEM</span><br>
 +
        <span style="color: black; font-size: 15px;font-weight: 600;">Tianjin University</span>
 +
    </div>
 +
        </a>
 +
    </div>
 +
        <div class="navbar-collapse navbar-right navbar-main-collapse collapse">
 +
        <ul class="nav navbar-nav navbar-right" style="margin-top: 15px;">
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Project
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Background">Background</a></li>
 +
                    <li><a href="#">Design</a></li>
 +
                    <li><a href="#">Perspective</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Experiment             
 +
                </a>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Modeling
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="#">Overview</a></li>
 +
                    <li><a href="#">Model | One</a></li>
 +
                    <li><a href="#">Model | Two</a></li>
 +
                    <li><a href="#">Integration</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Human Practice
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="#">Outreach</a></li>
 +
                    <li><a href="#">Consultation</a></li>
 +
                    <li><a href="#">Public Invesitigation</a></li>
 +
                    <li><a href="#">Colaboration</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Safety
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="#">Biosafety</a></li>
 +
                    <li><a href="#">Labsafety</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    About US
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="#">Members</a></li>
 +
                    <li><a href="#">Labs</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    Attribution
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="#">Teamwork</a></li>
 +
                    <li><a href="#">Project Support</a></li>
 +
                </ul>
 +
            </li>
 +
        </ul>
 +
    </div>
 +
 +
    <script type="text/javascript" src="https://2018.igem.org/Team:Tianjin/js/navigation?action=raw&ctype=text/javascript"></script>
 +
 +
 +
    </div>
 +
    </nav>
 +
 +
    <div class="container ">
 +
        <div class="row">
 +
            <div class="col-xs-12 titleBox">
 +
                <div class="title title-big">
 +
                    <p>Project Background</p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
        <div class="row partition">
 +
           
 +
        </div>
 +
        <div class="row">
 +
            <div class="col-xs-12 ">
 +
                <div class="title title-normal">
 +
                    <p></p>
 +
                </div>
 +
            </div>
 +
            <div class="col-xs-12 text">
 +
                <p>
 +
                In 2017, the Nobel Prize in Physiology or Medicine was awarded jointly to three Americans – Jeffrey Hall, Michael Rosbash and Michael Young – for their discoveries of molecular mechanisms controlling the circadian rhythm[1], which focused the public attention on the concept circadian once again . Circadian exists ubiquitously across the tree of life, regulating metabolic and behavioral changes in anticipation of day-night variations in environmental conditions[2]. Since the 4<sup>th</sup> century, when Androsthnenes[3] observed the diurnal and nocturnal variation in the movement of tamarind trees, the human race has never ceased to explore the mystery of circadian rhythm although the systematical study started only several decades ago. Today, we acquire the knowledge that cell-autonomous time-keeping system could be categorized into two types according to different clock mechanisms.
 +
                </p>
 +
            </div>
 +
        </div>
 +
 +
        <div class="row">
 +
            <div class="col-xs-12 partition">
 +
               
 +
            </div>
 +
        </div>
 +
 +
        <div class="row">
 +
            <div class="col-xs-12">
 +
                <div class="title title-normal">
 +
                    <p>Clock Mechanisms I: Transcription-Translation Feedback Loops</p>
 +
                </div>
 +
            </div>
 +
            <div class="col-lg-8 text">
 +
                <p>
 +
                  Transcription-Translation Feedback Loops（TTFL）mechanisms demonstrated that mRNA and protein oscillations control the anticipated behavioral changes of organisms. For example, in Neurospora crassa, transcription of the <i>Frq</i> gene is driven by the White Collar (WC) complex, comprised of the proteins Wc1 and Wc2. Subsequently, the FRQ protein interacts with RNA helicase, FRH, and this complex represses Frq transcription. The blue-light photoreceptor <i>Vvd</i> , which acts similarly to Drosophila Cry to promote clock protein degradation and modulate transcription, will interacts with both the repressing FRQ/FRH complex and the positive WC complex[4]. Today, the principle of TTFL is considered to be a universal bio-brick of circadian clocks and has been identified in all model systems studied to date[4]. 
 +
                </p>
 +
            </div>
 +
            <div class="col-lg-4 picture">
 +
                <img src="https://static.igem.org/mediawiki/2018/5/5d/T--Tianjin--doge.jpg">
 +
            </div>
 +
        </div>
 +
 +
        <div class="row">
 +
            <div class="col-xs-12">
 +
                <div class="title title-normal">
 +
                    <p>Clock Mechanisms II:Post-translational Feedback Loops</p>
 +
                </div>
 +
            </div>
 +
            <div class="col-xs-12 text">
 +
                <p>
 +
                  Apart from the regulation of transcripts and proteins, multiple circadian post-translational modifications of some components such as acetylation, methylation, sumoylation, and phosphorylations also proved to play important roles in circadian system or otherwise act as clocks themselves. A good example of this mechanism is the KaiABC oscillation system in the cyanobacteria. Oscillations are controlled by phosphorylation of the KaiC protein, which is modulated by the KaiA and KaiB proteins[5]. This mechanism is also used in the 2018 Tianjin iGem project and will be illustrated extensively in other pages.
 +
                </p>
 +
            </div>
 +
            <!--<div class="col-xs-4 picture">
 +
                <img src="img/1.jpg">
 +
            </div>-->
 +
        </div>
 +
    </div>
 +
 +
    <div id="footer" style="background-color: #111;text-align: center;">
 +
        <div class="container">
 +
            <div class="row">
 +
                <div class="col-sm-4 footer-part">
 +
                    <h4>
 +
                        Email
 +
                    </h4>
 +
                    <p>
 +
                        <i class="fa fa-envelope fa-lg"></i>
 +
                        hebo@tju.edu.cn
 +
                    </p>
 +
                </div>
 +
                <div class="col-sm-4 footer-part">
 +
                    <h4>
 +
                        Follow us
 +
                    </h4>
 +
                    <a href="https://twitter.com/iGEMTianjin2017" target="blank" id="facebook" style="padding: 0 0.5em 0 0;">
 +
                        <i class="fa fa-facebook-square fa-3x"></i>
 +
                    </a>
 +
                    <span></span>
 +
                    <a href="/https://twitter.com/iGEMTianjin2017" target="blank" id="twitter" style="padding: 0 0 0 0.5em;">
 +
                        <i class="fa fa-twitter-square fa-3x"></i>
 +
                    </a>
 +
                </div>
 +
                <div class="col-sm-4 footer-part">
 +
                    <h4>
 +
                        Contact us
 +
                    </h4>
 +
                    <p>
 +
                        <i class="fa fa-map-marker fa-lg"></i>
 +
                        Tianjin University
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
</body>
 +
</html>

Revision as of 08:31, 6 August 2018

Team:Tianjin - 2018.igem.org

Project Background

In 2017, the Nobel Prize in Physiology or Medicine was awarded jointly to three Americans – Jeffrey Hall, Michael Rosbash and Michael Young – for their discoveries of molecular mechanisms controlling the circadian rhythm[1], which focused the public attention on the concept circadian once again . Circadian exists ubiquitously across the tree of life, regulating metabolic and behavioral changes in anticipation of day-night variations in environmental conditions[2]. Since the 4th century, when Androsthnenes[3] observed the diurnal and nocturnal variation in the movement of tamarind trees, the human race has never ceased to explore the mystery of circadian rhythm although the systematical study started only several decades ago. Today, we acquire the knowledge that cell-autonomous time-keeping system could be categorized into two types according to different clock mechanisms.

Clock Mechanisms I: Transcription-Translation Feedback Loops

Transcription-Translation Feedback Loops（TTFL）mechanisms demonstrated that mRNA and protein oscillations control the anticipated behavioral changes of organisms. For example, in Neurospora crassa, transcription of the Frq gene is driven by the White Collar (WC) complex, comprised of the proteins Wc1 and Wc2. Subsequently, the FRQ protein interacts with RNA helicase, FRH, and this complex represses Frq transcription. The blue-light photoreceptor Vvd , which acts similarly to Drosophila Cry to promote clock protein degradation and modulate transcription, will interacts with both the repressing FRQ/FRH complex and the positive WC complex[4]. Today, the principle of TTFL is considered to be a universal bio-brick of circadian clocks and has been identified in all model systems studied to date[4].

Clock Mechanisms II:Post-translational Feedback Loops

Apart from the regulation of transcripts and proteins, multiple circadian post-translational modifications of some components such as acetylation, methylation, sumoylation, and phosphorylations also proved to play important roles in circadian system or otherwise act as clocks themselves. A good example of this mechanism is the KaiABC oscillation system in the cyanobacteria. Oscillations are controlled by phosphorylation of the KaiC protein, which is modulated by the KaiA and KaiB proteins[5]. This mechanism is also used in the 2018 Tianjin iGem project and will be illustrated extensively in other pages.