Difference between revisions of "Team:FSU/Description"

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{{FSU}}
 
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  <text class="cls-9" transform="translate(43.72 30.3)">Can <tspan x="7.22" y="0">C</tspan><tspan x="9.36" y="0">ells be </tspan><tspan x="21.74" y="0">C</tspan><tspan x="23.89" y="0">ont</tspan><tspan x="29.58" y="0">r</tspan><tspan x="31.1" y="0">ol</tspan><tspan x="34.43" y="0">l</tspan><tspan x="35.55" y="0">ed with Sound?</tspan></text>
 +
  <g>
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    <text class="cls-10" transform="translate(20 36.58)"><tspan class="cls-11">      </tspan><tspan x="4.38" y="0"> </tspan><tspan x="4.94" y="0">T</tspan><tspan x="6.13" y="0">oda</tspan><tspan x="10.58" y="0">y</tspan><tspan x="11.71" y="0">,</tspan><tspan class="cls-11" x="12.29" y="0"> it is </tspan><tspan x="17.31" y="0">r</tspan><tspan x="18.17" y="0">outine to induce the </tspan><tspan x="41.75" y="0">e</tspan><tspan x="43.16" y="0">xp</tspan><tspan x="45.96" y="0">r</tspan><tspan x="46.81" y="0">ession o</tspan><tspan x="56.22" y="0">f</tspan><tspan class="cls-11" x="57.02" y="0"> a gene in E</tspan><tspan x="70.3" y="0">.</tspan><tspan class="cls-11" x="70.88" y="0"> coli using only a small molecule</tspan><tspan x="107.51" y="0">.</tspan><tspan x="108.09" y="0"> </tspan><tspan x="0" y="3">R</tspan><tspan x="1.47" y="3">e</tspan><tspan x="2.88" y="3">al small</tspan><tspan x="11.38" y="3">.</tspan><tspan x="11.96" y="3"> </tspan><tspan x="12.52" y="3">T</tspan><tspan x="13.9" y="3">hink a</tspan><tspan x="20.67" y="3">r</tspan><tspan x="21.54" y="3">abinose</tspan><tspan x="30.62" y="3">,</tspan><tspan class="cls-11" x="31.19" y="3"> lactose</tspan><tspan x="39.89" y="3">,</tspan><tspan class="cls-11" x="40.47" y="3"> and rhamnose</tspan><tspan x="57.51" y="3">.</tspan><tspan x="58.09" y="3"> </tspan><tspan x="58.72" y="3">P</tspan><tspan x="59.99" y="3">ouring th</tspan><tspan x="70.4" y="3">r</tspan><tspan x="71.25" y="3">ough p</tspan><tspan x="79.48" y="3">r</tspan><tspan x="80.34" y="3">evious </tspan><tspan x="88.33" y="3">r</tspan><tspan x="89.18" y="3">es</tspan><tspan x="91.72" y="3">e</tspan><tspan x="93.14" y="3">a</tspan><tspan x="94.47" y="3">r</tspan><tspan x="95.33" y="3">ch</tspan><tspan x="98.03" y="3">,</tspan><tspan class="cls-11" x="98.61" y="3"> we’ve </tspan><tspan x="0" y="6">determined th</tspan><tspan x="16.33" y="6">a</tspan><tspan x="17.63" y="6">t di</tspan><tspan x="21.27" y="6">r</tspan><tspan x="22.13" y="6">ected soundwaves can st</tspan><tspan x="50.53" y="6">r</tspan><tspan x="51.38" y="6">ess a cell</tspan><tspan x="61.56" y="6">’</tspan><tspan x="61.99" y="6">s pumps to open</tspan><tspan x="81.24" y="6">.</tspan><tspan class="cls-11" x="81.82" y="6"> La</tspan><tspan x="85.05" y="6">r</tspan><tspan x="85.91" y="6">ge enough for our </tspan><tspan x="0" y="9">‘</tspan><tspan x="0.45" y="9">e</tspan><tspan x="1.87" y="9">xp</tspan><tspan x="4.66" y="9">r</tspan><tspan x="5.52" y="9">ession inducing’ molecules to pass inside the cell‘s walls</tspan><tspan x="69.17" y="9">.</tspan><tspan class="cls-11" x="0" y="12">        One may ask</tspan><tspan x="19.74" y="12">,</tspan><tspan class="cls-11" x="20.31" y="12"> can sound become a </tspan><tspan x="45.38" y="12">r</tspan><tspan x="46.24" y="12">outine m</tspan><tspan x="56.35" y="12">e</tspan><tspan x="57.76" y="12">ans to induce gene </tspan><tspan x="80.17" y="12">e</tspan><tspan x="81.59" y="12">xp</tspan><tspan x="84.39" y="12">r</tspan><tspan x="85.24" y="12">ession in enginee</tspan><tspan x="105.22" y="12">r</tspan><tspan x="106.08" y="12">ed </tspan><tspan x="0" y="15">E</tspan><tspan x="1.48" y="15">.</tspan><tspan class="cls-11" x="2.06" y="15"> coli?</tspan><tspan x="7.92" y="15"> </tspan><tspan x="8.51" y="15">While Sloveni</tspan><tspan x="24" y="15">a</tspan><tspan x="25.28" y="15">’</tspan><tspan x="25.71" y="15">s Sonicell and SUS</tspan><tspan x="46.63" y="15"> </tspan><tspan x="47.19" y="15">T</tspan><tspan x="48.38" y="15">ech</tspan><tspan x="52.51" y="15">’</tspan><tspan x="52.95" y="15">s </tspan><tspan x="54.68" y="15">C</tspan><tspan x="56.46" y="15">e</tspan><tspan x="57.87" y="15">arll</tspan><tspan x="61.36" y="15">’</tspan><tspan x="61.8" y="15">s Sec</tspan><tspan x="67.62" y="15">r</tspan><tspan x="68.48" y="15">et p</tspan><tspan x="72.91" y="15">r</tspan><tspan x="73.77" y="15">ojects pursued a similar </tspan><tspan x="0" y="18">question in mammalian cells</tspan><tspan x="32.31" y="18">,</tspan><tspan class="cls-11" x="32.89" y="18"> we’</tspan><tspan x="37.36" y="18">r</tspan><tspan x="38.22" y="18">e studying the details o</tspan><tspan x="64.69" y="18">f</tspan><tspan class="cls-11" x="65.49" y="18"> both p</tspan><tspan x="73.7" y="18">r</tspan><tspan x="74.55" y="18">ojects to induce </tspan><tspan class="cls-12" x="0" y="21">ch</tspan><tspan class="cls-12" x="2.68" y="21">r</tspan><tspan class="cls-12" x="3.54" y="21">omogenesis in enginee</tspan><tspan class="cls-12" x="30.45" y="21">r</tspan><tspan class="cls-12" x="31.32" y="21">ed E.</tspan><tspan class="cls-12" x="37.14" y="21">C</tspan><tspan class="cls-12" x="38.85" y="21">oli </tspan><tspan x="42.34" y="21">cells</tspan><tspan x="47.39" y="21">.</tspan><tspan class="cls-11" x="47.96" y="21"> In English</tspan><tspan x="59.65" y="21">,</tspan><tspan x="60.23" y="21"> </tspan><tspan x="60.85" y="21">we’ve caused ta</tspan><tspan x="79.13" y="21">r</tspan><tspan x="79.99" y="21">geted cells to change </tspan><tspan x="0" y="24">colors by b</tspan><tspan x="12.64" y="24">e</tspan><tspan x="14.06" y="24">aming sound </tspan><tspan x="29.7" y="24">a</tspan><tspan x="31.03" y="24">t them</tspan><tspan x="38.41" y="24">.</tspan><tspan x="38.98" y="24"> </tspan></text>
 +
    <text class="cls-13" transform="translate(20 36.58)"><tspan class="cls-11">      </tspan><tspan x="4.38" y="0"> </tspan><tspan x="4.94" y="0">T</tspan><tspan x="6.13" y="0">oda</tspan><tspan x="10.58" y="0">y</tspan><tspan x="11.71" y="0">,</tspan><tspan class="cls-11" x="12.29" y="0"> it is </tspan><tspan x="17.31" y="0">r</tspan><tspan x="18.17" y="0">outine to induce the </tspan><tspan x="41.75" y="0">e</tspan><tspan x="43.16" y="0">xp</tspan><tspan x="45.96" y="0">r</tspan><tspan x="46.81" y="0">ession o</tspan><tspan x="56.22" y="0">f</tspan><tspan class="cls-11" x="57.02" y="0"> a gene in E</tspan><tspan x="70.3" y="0">.</tspan><tspan class="cls-11" x="70.88" y="0"> coli using only a small molecule</tspan><tspan x="107.51" y="0">.</tspan><tspan x="108.09" y="0"> </tspan><tspan x="0" y="3">R</tspan><tspan x="1.47" y="3">e</tspan><tspan x="2.88" y="3">al small</tspan><tspan x="11.38" y="3">.</tspan><tspan x="11.96" y="3"> </tspan><tspan x="12.52" y="3">T</tspan><tspan x="13.9" y="3">hink a</tspan><tspan x="20.67" y="3">r</tspan><tspan x="21.54" y="3">abinose</tspan><tspan x="30.62" y="3">,</tspan><tspan class="cls-11" x="31.19" y="3"> lactose</tspan><tspan x="39.89" y="3">,</tspan><tspan class="cls-11" x="40.47" y="3"> and rhamnose</tspan><tspan x="57.51" y="3">.</tspan><tspan x="58.09" y="3"> </tspan><tspan x="58.72" y="3">P</tspan><tspan x="59.99" y="3">ouring th</tspan><tspan x="70.4" y="3">r</tspan><tspan x="71.25" y="3">ough p</tspan><tspan x="79.48" y="3">r</tspan><tspan x="80.34" y="3">evious </tspan><tspan x="88.33" y="3">r</tspan><tspan x="89.18" y="3">es</tspan><tspan x="91.72" y="3">e</tspan><tspan x="93.14" y="3">a</tspan><tspan x="94.47" y="3">r</tspan><tspan x="95.33" y="3">ch</tspan><tspan x="98.03" y="3">,</tspan><tspan class="cls-11" x="98.61" y="3"> we’ve </tspan><tspan x="0" y="6">determined th</tspan><tspan x="16.33" y="6">a</tspan><tspan x="17.63" y="6">t di</tspan><tspan x="21.27" y="6">r</tspan><tspan x="22.13" y="6">ected soundwaves can st</tspan><tspan x="50.53" y="6">r</tspan><tspan x="51.38" y="6">ess a cell</tspan><tspan x="61.56" y="6">’</tspan><tspan x="61.99" y="6">s pumps to open</tspan><tspan x="81.24" y="6">.</tspan><tspan class="cls-11" x="81.82" y="6"> La</tspan><tspan x="85.05" y="6">r</tspan><tspan x="85.91" y="6">ge enough for our </tspan><tspan x="0" y="9">‘</tspan><tspan x="0.45" y="9">e</tspan><tspan x="1.87" y="9">xp</tspan><tspan x="4.66" y="9">r</tspan><tspan x="5.52" y="9">ession inducing’ molecules to pass inside the cell‘s walls</tspan><tspan x="69.17" y="9">.</tspan><tspan class="cls-11" x="0" y="12">        One may ask</tspan><tspan x="19.74" y="12">,</tspan><tspan class="cls-11" x="20.31" y="12"> can sound become a </tspan><tspan x="45.38" y="12">r</tspan><tspan x="46.24" y="12">outine m</tspan><tspan x="56.35" y="12">e</tspan><tspan x="57.76" y="12">ans to induce gene </tspan><tspan x="80.17" y="12">e</tspan><tspan x="81.59" y="12">xp</tspan><tspan x="84.39" y="12">r</tspan><tspan x="85.24" y="12">ession in enginee</tspan><tspan x="105.22" y="12">r</tspan><tspan x="106.08" y="12">ed </tspan><tspan x="0" y="15">E</tspan><tspan x="1.48" y="15">.</tspan><tspan class="cls-11" x="2.06" y="15"> coli?</tspan><tspan x="7.92" y="15"> </tspan><tspan x="8.51" y="15">While Sloveni</tspan><tspan x="24" y="15">a</tspan><tspan x="25.28" y="15">’</tspan><tspan x="25.71" y="15">s Sonicell and SUS</tspan><tspan x="46.63" y="15"> </tspan><tspan x="47.19" y="15">T</tspan><tspan x="48.38" y="15">ech</tspan><tspan x="52.51" y="15">’</tspan><tspan x="52.95" y="15">s </tspan><tspan x="54.68" y="15">C</tspan><tspan x="56.46" y="15">e</tspan><tspan x="57.87" y="15">arll</tspan><tspan x="61.36" y="15">’</tspan><tspan x="61.8" y="15">s Sec</tspan><tspan x="67.62" y="15">r</tspan><tspan x="68.48" y="15">et p</tspan><tspan x="72.91" y="15">r</tspan><tspan x="73.77" y="15">ojects pursued a similar </tspan><tspan x="0" y="18">question in mammalian cells</tspan><tspan x="32.31" y="18">,</tspan><tspan class="cls-11" x="32.89" y="18"> we’</tspan><tspan x="37.36" y="18">r</tspan><tspan x="38.22" y="18">e studying the details o</tspan><tspan x="64.69" y="18">f</tspan><tspan class="cls-11" x="65.49" y="18"> both p</tspan><tspan x="73.7" y="18">r</tspan><tspan x="74.55" y="18">ojects to induce </tspan><tspan class="cls-12" x="0" y="21">ch</tspan><tspan class="cls-12" x="2.68" y="21">r</tspan><tspan class="cls-12" x="3.54" y="21">omogenesis in enginee</tspan><tspan class="cls-12" x="30.45" y="21">r</tspan><tspan class="cls-12" x="31.32" y="21">ed E.</tspan><tspan class="cls-12" x="37.14" y="21">C</tspan><tspan class="cls-12" x="38.85" y="21">oli </tspan><tspan x="42.34" y="21">cells</tspan><tspan x="47.39" y="21">.</tspan><tspan class="cls-11" x="47.96" y="21"> In English</tspan><tspan x="59.65" y="21">,</tspan><tspan x="60.23" y="21"> </tspan><tspan x="60.85" y="21">we’ve caused ta</tspan><tspan x="79.13" y="21">r</tspan><tspan x="79.99" y="21">geted cells to change </tspan><tspan x="0" y="24">colors by b</tspan><tspan x="12.64" y="24">e</tspan><tspan x="14.06" y="24">aming sound </tspan><tspan x="29.7" y="24">a</tspan><tspan x="31.03" y="24">t them</tspan><tspan x="38.41" y="24">.</tspan><tspan x="38.98" y="24"> </tspan></text>
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Revision as of 00:49, 7 August 2018

description01 Can Cells be Controlled with Sound? Today, it is routine to induce the expression of a gene in E. coli using only a small molecule. Real small. Think arabinose, lactose, and rhamnose. Pouring through previous research, we’ve determined that directed soundwaves can stress a cell’s pumps to open. Large enough for our ‘expression inducing’ molecules to pass inside the cell‘s walls. One may ask, can sound become a routine means to induce gene expression in engineered E. coli? While Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question in mammalian cells, we’re studying the details of both projects to induce chromogenesis in engineered E.Coli cells. In English, we’ve caused targeted cells to change colors by beaming sound at them. Today, it is routine to induce the expression of a gene in E. coli using only a small molecule. Real small. Think arabinose, lactose, and rhamnose. Pouring through previous research, we’ve determined that directed soundwaves can stress a cell’s pumps to open. Large enough for our ‘expression inducing’ molecules to pass inside the cell‘s walls. One may ask, can sound become a routine means to induce gene expression in engineered E. coli? While Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question in mammalian cells, we’re studying the details of both projects to induce chromogenesis in engineered E.Coli cells. In English, we’ve caused targeted cells to change colors by beaming sound at them.

Project Description

Question
Can sound be used to induce gene expression in engineered E. coli?

Approach
Today, it is routine to induce the expression of a gene in E. coli using a small molecule. Examples are arabinose, lactose, and rhamnose. Can sound become a routine means to induce gene expression in engineered E. coli? Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells. We are studying the details of both projects. We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound. In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound. We will identify promoters that seem to be induced by pathways that respond to sound. The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds. We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.

Impact
If we are successful in using sound to induce gene express in E. coli, the impact is unclear. Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression. We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.

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