Difference between revisions of "Team:NUS Singapore-Sci/Wetlab/Interlab"

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This year, our team took part in the 2018 InterLab Study, which aims to standardise the fluorescence levels of bacteria transformed with different devices conferring it fluorescence. This is accomplished through the use of a plate reader. The plate reader we used was Synergy H1 Hybrid Multi-Mode Reader (courtesy of SynCTI?), which could read both absorbance and fluorescence.  
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This year, our team took part in the 2018 InterLab Study, which aims to standardise the fluorescence levels of bacteria transformed with different devices conferring it fluorescence. This is accomplished through the use of a plate reader. The plate reader we used was Synergy H1 Hybrid Multi-Mode Reader (courtesy of SynCTI?), which could read both absorbance and fluorescence. <br>
  
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For all the following measurements, we did not use pathlength correction, and kept the temperature setting to room temperature (20-25°C). The bandpass width is fixed at 16 nm. Bottom optics are used.
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For all the following measurements, we did not use pathlength correction, and kept the temperature setting to room temperature (20-25°C). The bandpass width is fixed at 16 nm. Bottom optics are used. <br>
  
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Before cell measurements were taken, three different calibration protocols were done.
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Before cell measurements were taken, three different calibration protocols were done. <br>
  
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OD600 Reference Point - LUDOX Protocol
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1) OD600 Reference Point - LUDOX Protocol <br>
  
 
LUDOX CL-X (45% colloidal silica suspension) was used to convert the Absorbance at 600 nm as obtained from the plate reader into a comparable OD600 measurement as obtained from a spectrophotometer. Here, pathlength correction is especially important to be switched off so as to not provide correction for the volume of sample in the 96-well plate.
 
LUDOX CL-X (45% colloidal silica suspension) was used to convert the Absorbance at 600 nm as obtained from the plate reader into a comparable OD600 measurement as obtained from a spectrophotometer. Here, pathlength correction is especially important to be switched off so as to not provide correction for the volume of sample in the 96-well plate.

Revision as of 19:13, 7 August 2018

NUS Singapore Science: About Us! This year, our team took part in the 2018 InterLab Study, which aims to standardise the fluorescence levels of bacteria transformed with different devices conferring it fluorescence. This is accomplished through the use of a plate reader. The plate reader we used was Synergy H1 Hybrid Multi-Mode Reader (courtesy of SynCTI?), which could read both absorbance and fluorescence.
For all the following measurements, we did not use pathlength correction, and kept the temperature setting to room temperature (20-25°C). The bandpass width is fixed at 16 nm. Bottom optics are used.
Before cell measurements were taken, three different calibration protocols were done.
1) OD600 Reference Point - LUDOX Protocol
LUDOX CL-X (45% colloidal silica suspension) was used to convert the Absorbance at 600 nm as obtained from the plate reader into a comparable OD600 measurement as obtained from a spectrophotometer. Here, pathlength correction is especially important to be switched off so as to not provide correction for the volume of sample in the 96-well plate.