Difference between revisions of "Template:Virginia/index"
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<h1 id="project-description">Project Description</h1> | <h1 id="project-description">Project Description</h1> | ||
| − | <p>Heterogeneity of cell populations caused by <span class="tooltip">quorum sensing<span class="shortdef"> | + | <h2 id="test-subheading">Test subheading</h2> |
| − | <p>A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobricks containing pLsr, T7 RNA Polymerase, LsrK, LsrACDB, LuxS, YdgG, and superfolding Green Fluorescent Protein (sfGFP) enables both the enhancement of natural quorum sensing and the quantification of protein activation among the bacteria in a colony. This device will improve the viability of autoinduction as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes and | + | <p>Heterogeneity of cell populations caused by <span class="tooltip">quorum sensing<span class="shortdef">In biology, quorum sensing is the ability to detect and to respond to cell population density by gene regulation.</span><span class="longdef" tabindex="1">Long Def</span> </span> leads to variability in gene expression that is hard to predict. During biomanufacturing, elevating quorum-induced protein expression will lead to gain of profit. Decreasing this expression can also be beneficial in situations where undesirable <span class="tooltip">biofilms<span class="shortdef">ggregate of microorganisms in which cells that are frequently embedded within a self-produced matrix of extracellular polymeric substances (EPSs) adhere to each other and/or to a surface.</span><span class="longdef" tabindex="1">LD</span> </span> may form on medical equipment or controlling <span class="tooltip">virulence<span class="shortdef">Virulence provides a quantitative measure of the pathogenicity or the likelihood of a microbe to cause disease.</span><span class="longdef" tabindex="1">LD</span> </span> in bacteria. We will modify the existing bacterial quorum sensing system controlled by the <span class="tooltip">Lsr operon<span class="shortdef">Short Def</span><span class="longdef" tabindex="1">Long Def</span> </span> by upregulating the synthesis and excretion of <span class="tooltip">Autoinducer-2<span class="shortdef">A member of a family of signaling molecules used in quorum sensing.</span><span class="longdef" tabindex="1">LD</span> </span>, a universal quorum molecule. This will increase population-scale AI-2 intake and <span class="tooltip">phosphorylation<span class="shortdef">A biochemical process that involves the addition of phosphate to an organic compound. </span><span class="longdef" tabindex="1">LD</span> </span> after the initial AI-2 threshold concentrations have been reached to reduce variability in induced gene expression.</p> |
| + | <h2 id="another-test-subheading">Another test subheading</h2> | ||
| + | <p>A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobricks containing <span class="tooltip">pLsr<span class="shortdef">A promoter of the Lsr Operon</span><span class="longdef" tabindex="1">LD</span> </span>, <span class="tooltip">T7 RNA Polymerase<span class="shortdef">A RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction.</span><span class="longdef" tabindex="1">LD</span> </span>, <span class="tooltip">LsrK<span class="shortdef">SD</span><span class="longdef" tabindex="1">LD</span> </span>, <span class="tooltip">LsrACDB<span class="shortdef">SD</span><span class="longdef" tabindex="1">LD</span> </span>, <span class="tooltip">LuxS<span class="shortdef">SD</span><span class="longdef" tabindex="1">LD</span> </span>, <span class="tooltip">YdgG<span class="shortdef">SD</span><span class="longdef" tabindex="1">LD</span> </span>, and superfolding Green Fluorescent Protein (<span class="tooltip">sfGFP<span class="shortdef">SD</span><span class="longdef" tabindex="1">LD</span> </span>) enables both the enhancement of natural quorum sensing and the quantification of protein activation among the bacteria in a colony. This device will improve the viability of <span class="tooltip">autoinduction<span class="shortdef">The induction of something by itself or without external stimuli.</span><span class="longdef" tabindex="1">LD</span> </span> as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes and increasing expression within a single culture to reduce costs leading to an increase in profits. In addition, by insertion of T7 polymerase into a quorum-sensitive region of the genome of E. coli, we will create a chassis with specific, customizable quorum response for engineering and scientific applications.</p> | ||
| + | <h1 id="a-second-article">A second article</h1> | ||
| + | <p>Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.</p> | ||
| + | <h2 id="so-many-subheadings">so many subheadings</h2> | ||
| + | <p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec hendrerit tempor tellus. Donec pretium posuere tellus. Proin quam nisl, tincidunt et, mattis eget, convallis nec, purus. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nulla posuere. Donec vitae dolor. Nullam tristique diam non turpis. Cras placerat accumsan nulla. Nullam rutrum. Nam vestibulum accumsan nisl.</p> | ||
| + | <p>Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.</p> | ||
| + | <h2 id="so-many-subheadings-">so many subheadings!!!</h2> | ||
| + | <p>Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.</p> | ||
| + | <p>Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.</p> | ||
Revision as of 14:37, 15 August 2018
Contents
Project Description
Test subheading
Heterogeneity of cell populations caused by quorum sensingIn biology, quorum sensing is the ability to detect and to respond to cell population density by gene regulation.Long Def leads to variability in gene expression that is hard to predict. During biomanufacturing, elevating quorum-induced protein expression will lead to gain of profit. Decreasing this expression can also be beneficial in situations where undesirable biofilmsggregate of microorganisms in which cells that are frequently embedded within a self-produced matrix of extracellular polymeric substances (EPSs) adhere to each other and/or to a surface.LD may form on medical equipment or controlling virulenceVirulence provides a quantitative measure of the pathogenicity or the likelihood of a microbe to cause disease.LD in bacteria. We will modify the existing bacterial quorum sensing system controlled by the Lsr operonShort DefLong Def by upregulating the synthesis and excretion of Autoinducer-2A member of a family of signaling molecules used in quorum sensing.LD , a universal quorum molecule. This will increase population-scale AI-2 intake and phosphorylationA biochemical process that involves the addition of phosphate to an organic compound. LD after the initial AI-2 threshold concentrations have been reached to reduce variability in induced gene expression.
Another test subheading
A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobricks containing pLsrA promoter of the Lsr OperonLD , T7 RNA PolymeraseA RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction.LD , LsrKSDLD , LsrACDBSDLD , LuxSSDLD , YdgGSDLD , and superfolding Green Fluorescent Protein (sfGFPSDLD ) enables both the enhancement of natural quorum sensing and the quantification of protein activation among the bacteria in a colony. This device will improve the viability of autoinductionThe induction of something by itself or without external stimuli.LD as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes and increasing expression within a single culture to reduce costs leading to an increase in profits. In addition, by insertion of T7 polymerase into a quorum-sensitive region of the genome of E. coli, we will create a chassis with specific, customizable quorum response for engineering and scientific applications.
A second article
Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.
so many subheadings
Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec hendrerit tempor tellus. Donec pretium posuere tellus. Proin quam nisl, tincidunt et, mattis eget, convallis nec, purus. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nulla posuere. Donec vitae dolor. Nullam tristique diam non turpis. Cras placerat accumsan nulla. Nullam rutrum. Nam vestibulum accumsan nisl.
Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.
so many subheadings!!!
Aliquam erat volutpat. Nunc eleifend leo vitae magna. In id erat non orci commodo lobortis. Proin neque massa, cursus ut, gravida ut, lobortis eget, lacus. Sed diam. Praesent fermentum tempor tellus. Nullam tempus. Mauris ac felis vel velit tristique imperdiet. Donec at pede. Etiam vel neque nec dui dignissim bibendum. Vivamus id enim. Phasellus neque orci, porta a, aliquet quis, semper a, massa. Phasellus purus. Pellentesque tristique imperdiet tortor. Nam euismod tellus id erat.
Nullam eu ante vel est convallis dignissim. Fusce suscipit, wisi nec facilisis facilisis, est dui fermentum leo, quis tempor ligula erat quis odio. Nunc porta vulputate tellus. Nunc rutrum turpis sed pede. Sed bibendum. Aliquam posuere. Nunc aliquet, augue nec adipiscing interdum, lacus tellus malesuada massa, quis varius mi purus non odio. Pellentesque condimentum, magna ut suscipit hendrerit, ipsum augue ornare nulla, non luctus diam neque sit amet urna. Curabitur vulputate vestibulum lorem. Fusce sagittis, libero non molestie mollis, magna orci ultrices dolor, at vulputate neque nulla lacinia eros. Sed id ligula quis est convallis tempor. Curabitur lacinia pulvinar nibh. Nam a sapien.