Difference between revisions of "Team:UCSC/Experiments"

Line 34: Line 34:
 
       <p class="p-title">Experiment 0</p>
 
       <p class="p-title">Experiment 0</p>
 
       <p> Description in progress</p>
 
       <p> Description in progress</p>
−
       <img src="https://static.igem.org/mediawiki/2018/6/61/T--UCSC--YarrowiaPathway.jpg" width="80%" class="image-inpage">
+
       <img src="https://static.igem.org/mediawiki/2018/2/2d/T--UCSC--Experiment_0_Overview.jpg" width="80%" class="image-inpage">
−
       <p class="image-inpage-caption" style="width:80%; font-size:85% !important"> Figure 1: The pathway above shows one of the products of our strain of Yarrowia lipolytica, Ergosterol, and the pathway following to produce progesterone. * Two genes, FDX1 and ADR, are also required to be inserted in the metabloic shunt for Yarrowia to produce Pregnenolone.</p>
+
       <p class="image-inpage-caption" style="width:66%; font-size:85% !important"> Figure 1: Creation of pOPPY-UC19-yXXU containing URA3, pUC19 backbone, Lox sites and homologous arms using Gibson Assembly. Transformation of Y. lipolytica to create Y. lipolytica str. LipLox homologous recombination. </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 44: Line 44:
 
       <p class="p-title">Experiment 1</p>
 
       <p class="p-title">Experiment 1</p>
 
       <p> Description in progress</p>
 
       <p> Description in progress</p>
 +
      <img src="https://static.igem.org/mediawiki/2018/8/80/T--UCSC--Experiment_1_Overview.jpg" width="80%" class="image-inpage">
 +
      <p class="image-inpage-caption" style="width:66%; font-size:85% !important"> Figure 2: Creation of pOPPY-19-yP via overlap extension PCR of genes involved in progesterone biosynthesis and gibson assembly of this gene cassette with homologous arms and linearized pUC19 backbone. Transformation of Y. lipolytica str. LipLox using homologous recombination to exchange gene cassette. Selection of Y. lipolytica str. PoPPY using 5FOA URA+.</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>

Revision as of 18:33, 16 August 2018

Experiments

Overview

Description in progress

divider image

Experiment 0

Description in progress

Figure 1: Creation of pOPPY-UC19-yXXU containing URA3, pUC19 backbone, Lox sites and homologous arms using Gibson Assembly. Transformation of Y. lipolytica to create Y. lipolytica str. LipLox homologous recombination.

Experiment 1

Description in progress

Figure 2: Creation of pOPPY-19-yP via overlap extension PCR of genes involved in progesterone biosynthesis and gibson assembly of this gene cassette with homologous arms and linearized pUC19 backbone. Transformation of Y. lipolytica str. LipLox using homologous recombination to exchange gene cassette. Selection of Y. lipolytica str. PoPPY using 5FOA URA+.

Experiment 2

Description in progress

Experiment 3

Description in progress

Quantification

Description in progress