Difference between revisions of "Team:NTU-Singapore"

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{{NTU-Singapore}}
 
 
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<script src="https://2018.igem.org/wiki/index.php?title=Team:NTU-Singapore/Test_Page/JQJS&action=raw&ctype=text/javascript"></script>
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<script src="https://2018.igem.org/wiki/index.php?title=Team:NTU-Singapore/Test_Page/BootstrapJS&action=raw&ctype=text/javascript"></script>
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<script src="https://2018.igem.org/wiki/index.php?title=Team:NTU-Singapore/Test_Page/BlocsJS&action=raw&ctype=text/javascript"></script>
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    <title>Home</title>
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/***************************************************** DEFAULT WIKI SETTINGS  ***************************************/
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/* Clear the default wiki settings */
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  #content { width:100%; padding:0px;  margin-top:-20px; margin-left:0px;}
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<source src="img/lazyload-ph.png" data-src="https://static.igem.org/mediawiki/2018/6/69/T--NTU-Singapore--DNA_Hero_Background.mp4" type="video/mp4" />
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<a class="navbar-brand logo-margin" href="index.html"><img src="img/lazyload-ph.png" data-src="https://static.igem.org/mediawiki/2018/2/2b/T--NTU-Singapore--Logo.png" alt="logo" width="150" height="42" class="lazyload" /></a>
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<button id="nav-toggle" type="button" class="ui-navbar-toggle navbar-toggle menu-icon-uneven-b-list" data-toggle="collapse" data-target=".navbar-1">
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<h1> Welcome to iGEM 2018! </h1>
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<span class="sr-only">Toggle navigation</span><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</button>
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</div>
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<div class="collapse navbar-collapse navbar-1 nav-special sidebar-nav">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" aria-expanded="false">Team<span class="caret"></span></a>
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<ul class="dropdown-menu" role="menu">
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<h3>Before you start</h3>
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<li>
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<p> Please read the following pages:</p>
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<a href="index.html">Our Team</a>
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<ul>
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</li>
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<a href="index.html">Collaborations</a>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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</li>
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</ul>
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</ul>
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</div>
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</li>
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<li>
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<div class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" aria-expanded="false">Project<span class="caret"></span></a>
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<ul class="dropdown-menu" role="menu">
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<li>
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<a href="index.html">DNA Editor</a>
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</li>
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<li>
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<a href="index.html">REPAIR</a>
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</li>
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<li>
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<a href="index.html">Nanopore</a>
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</li>
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<li>
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<a href="index.html">Demostrate</a>
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</li>
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<li>
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<a href="index.html">Improve</a>
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</li>
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<li>
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<a href="index.html">Attributions</a>
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</li>
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</ul>
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</div>
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</li>
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<li>
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<a href="interlab-measurement.html">InterLab</a>
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</li>
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<li>
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<div class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" aria-expanded="false">Parts<span class="caret"></span></a>
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<ul class="dropdown-menu" role="menu">
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<li>
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<a href="index.html">Parts Overview</a>
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</li>
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<li>
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<a href="index.html">Basic Parts</a>
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</li>
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<li>
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<a href="index.html">Composite Parts</a>
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</li>
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</ul>
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</div>
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</li>
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<li>
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<div class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" aria-expanded="false">Human Practices<span class="caret"></span></a>
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<ul class="dropdown-menu" role="menu">
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<li>
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<a href="index.html">Overview</a>
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</li>
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<li>
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<a href="index.html">Engagement &amp; Outreach</a>
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</li>
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<li>
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<a href="index.html">Integrated Human Practice</a>
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</li>
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</ul>
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</div>
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</li>
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<li>
 +
<div class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" aria-expanded="false">Awards<span class="caret"></span></a>
 +
<ul class="dropdown-menu" role="menu">
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<li>
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<a href="index.html">Metal Criteria</a>
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</li>
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<li>
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<a href="index.html">Best Human Practice</a>
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</li>
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</ul>
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</div>
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</li>
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<li>
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<a href="index.html" target="_blank">Judging Form</a>
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</li>
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</ul>
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</div>
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</nav>
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</div>
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<div class="container">
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<div class="row picture-margin ">
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<div class="col-sm-12">
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<img src="img/lazyload-ph.png" data-src="https://static.igem.org/mediawiki/2018/3/3e/T--NTU-Singapore--Logo_Eye.png" class="center-block mg-sm hero-break  img-responsive lazyload" width="500" srcset="img/Eye%20Structure.png 2x, img/Eye%20Structure.png 3x" /><img src="img/lazyload-ph.png" data-src="https://static.igem.org/mediawiki/2018/2/2b/T--NTU-Singapore--Logo.png" class="center-block mg-lg img-responsive logo-break lazyload" width="218" height="38" />
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<h1 class="text-center mg-lg hero-bloc-text tc-white ">
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<span style="font-size:80%;">A CRISPR/Cas Base-Editing Toolkit </span>
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</h1>
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<h3 class="text-center mg-lg hero-bloc-text-sub tc-white ">
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From Genome to Transcriptome, From Detection to Modification.
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</h3>
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<div class="text-center">
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<a class="btn btn-rd wire-btn-white btn-wire -extra-break btn-lg" onclick="scrollToTarget('#text-1')">Our Story</a>
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</div>
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</div>
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</div>
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</div>
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<div class="container fill-bloc-bottom-edge">
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<div class="row row-no-gutters">
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<div class="col-sm-12">
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<div class="text-center">
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<a onclick="scrollToTarget('#text-1')"><span class="fa fa-angle-double-down icon-md"></span></a>
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</div>
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</div>
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</div>
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</div>
 
</div>
 
</div>
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<div class="clear extra_space"></div>
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<div class="bloc none bloc-fill-screen full-width-bloc bg-T--NTU-Singapore--DNA-Base-Editor-Bg bgc-white l-bloc" id="text-1">
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<div class="line_divider"></div>
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<div class="container">
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<div class="clear extra_space"></div>
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<div class="row row-margin">
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<div class="col-sm-6 col-sm-push-6">
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</div>
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<div class="col-sm-6 col-sm-pull-6">
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<div class="column full_size" >
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<h2 class="mg-lg tc-black  text-left">
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<h3> Styling your wiki </h3>
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<span style="font-size:80%;">A Continuation and Beyond.</span>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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</h2>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h3 class=" mg-md text-left">
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<span style="font-size:80%;">CRISPR/Cas9 DNA Base Editor</span>
 +
</h3>
 +
<p class=" mg-lg text-left">
 +
<div style="text-align: left;"><div style="line-height: 200%;">With the successful truncated dCas9-mediated transcriptional activation last year, we moved further this year into DNA base editing using CRISPR/Cas9 system. We also improved our truncated dCas9 further with novel strategies for higher binding efficiency.</div></div>
 +
</p><a href="index.html" class="btn btn-wire btn-rd pull-left btn-lg" style="margin-top: 5%;">Project Improve</a>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="container fill-bloc-bottom-edge">
 +
<div class="row">
 +
<div class="col-sm-12">
 +
<div class="text-center">
 +
<a onclick="scrollToTarget('#bloc-3')"><span class="fa fa-angle-double-down icon-md"></span></a>
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</div>
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</div>
 +
</div>
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</div>
 
</div>
 
</div>
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<!-- text-1 END -->
  
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<!-- bloc-3 -->
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<div class="bloc full-width-bloc bloc-fill-screen bg-T--NTU-Singapore--IHP-Bg bgc-white l-bloc" id="bloc-3">
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<div class="container">
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<div class="clear extra_space"></div>
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<div class="row row-margin">
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<div class="col-sm-6">
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</div>
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<div class="col-sm-6">
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<div class="column third_size" >
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<h2 class="ipad-bloc-text-vc tc-black  mg-lg">
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<span style="font-size:80%;">Human-Centred Research</span>
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<h3> Uploading pictures and files </h3>
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</h2>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p class="mg-lg  text-left">
−
 
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<div style="text-align: left;"><div style="line-height: 200%;">Our research involves not only the students and scientists, but also the Singapore public. While doing our project, we looked into the public opinions towards gene editing. Our human practice tells how&nbsp;our project evolved around the local community that we live in.&nbsp;</div></div>
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</p><a href="index.html" class="btn btn-wire btn-rd" style="margin-top: 5%;">Integrated Human Practice</a>
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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</div>
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<div class="button_link">
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</div>
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<a href="https://2018.igem.org/Special:Upload">
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<div class="container fill-bloc-bottom-edge">
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UPLOAD FILES
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<div class="row">
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</a>
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<div class="col-sm-12">
 +
<div class="text-center">
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<a onclick="scrollToTarget('#bloc-4')"><span class="fa fa-angle-double-down icon-md"></span></a>
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</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
 +
<!-- bloc-3 END -->
  
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<!-- bloc-4 -->
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<div class="bloc bgc-white l-bloc bloc-fill-screen full-width-bloc" id="bloc-4">
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<div class="container">
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<div class="row row-margin">
 +
<div class="col-sm-6 col-sm-push-6">
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</div>
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<div class="col-sm-6 col-sm-pull-6">
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<h2 class="mg-lg  tc-black text-left">
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<span style="font-size:80%;">Adapt to Change.</span>
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</h2>
 +
<h3 class="mg-md text-left">
 +
<span style="font-size:80%;">CRISPR/Cas13b RNA Base Editor</span>
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</h3>
 +
<p class="mg-lg  text-left">
 +
<div style="text-align: left;"><div style="line-height: 200%;">As we have learnt through our interviews with medical doctors and interaction with the public, it is evident that RNA editing is more promising and better accep-ted in our society. Hence, we reflected on our project and ventured into RNA editing.</div></div>
 +
</p><a href="index.html" class="btn btn-wire btn-rd pull-left">Project REPAIR</a>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="container fill-bloc-bottom-edge">
 +
<div class="row">
 +
<div class="col-sm-12">
 +
<div class="text-center">
 +
<a onclick="scrollToTarget('#bloc-5')"><span class="fa fa-angle-double-down icon-md"></span></a>
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</div>
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</div>
 +
</div>
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</div>
 
</div>
 
</div>
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<!-- bloc-4 END -->
  
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<div class="column third_size" >
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<!-- bloc-5 -->
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<h3> Wiki template information </h3>
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<div class="bloc bloc-fill-screen full-width-bloc b-parallax bg-T--NTU-Singapore--Nanopore-Bg bgc-white l-bloc" id="bloc-5">
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
<div class="container">
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<div class="row row-margin">
−
</div>  
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<div class="col-sm-6">
−
 
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<img src="img/lazyload-ph.png" data-src="https://static.igem.org/mediawiki/2018/1/10/T--NTU-Singapore--Nanopore_Graphics.png" class="picture-margin img-responsive leftpics lazyload" />
−
 
+
</div>
−
 
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<div class="col-sm-6">
−
<div class="column third_size" >
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<h2 class="mg-lg  tc-black">
−
<div class="highlight decoration_B_full">
+
<span style="font-size:80%;">Aim Even Higher.</span>
−
<h3> Editing your wiki </h3>
+
</h2>
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
<h3 class=" mg-md">
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
<span style="font-size:80%;">Modification-Sensitive&nbsp;Nanopore Sequencing</span>
−
 
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</h3>
−
<div class="button_link">
+
<p class="mg-lg ">
−
<a href="https://2018.igem.org/wiki/index.php?title=Team:NTU-Singapore&action=edit">  
+
<div style="text-align: left;"><div style="line-height: 200%;">To address the significant concern around the safety of RNA editing technology, we designed an unbiased, accurate RNA sequencing tool. Expanding the nanopore sequencing platform, we hope to enhance the saftey of RNA editing.</div></div>
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EDIT PAGE
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</p><a href="index.html" class="btn btn-wire btn-rd pull-left" style="margin-top: 5%;">Project Nanopore</a>
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</a>
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</div>
 +
</div>
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</div>
 
</div>
 
</div>
 +
<!-- bloc-5 END -->
  
 +
<!-- ScrollToTop Button -->
 +
<a class="bloc-button btn btn-d scrollToTop" onclick="scrollToTarget('1')"><span class="fa fa-chevron-up"></span></a>
 +
<!-- ScrollToTop Button END-->
  
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</div>
 
  
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<div class="clear extra_space"></div>
 
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<div class="line_divider"></div>
 
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<div class="clear extra_space"></div>
 
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<div class="column two_thirds_size" >
 
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<h3>Tips</h3>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
−
<li>Have lots of fun! </li>
 
−
</ul>
 
 
</div>
 
</div>
 +
<!-- Main container END -->
  
  
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<div class="column third_size">
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</body>
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
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</div>
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Revision as of 17:39, 23 September 2018

Home

A Continuation and Beyond.

CRISPR/Cas9 DNA Base Editor

With the successful truncated dCas9-mediated transcriptional activation last year, we moved further this year into DNA base editing using CRISPR/Cas9 system. We also improved our truncated dCas9 further with novel strategies for higher binding efficiency.

Project Improve

Human-Centred Research

Our research involves not only the students and scientists, but also the Singapore public. While doing our project, we looked into the public opinions towards gene editing. Our human practice tells how our project evolved around the local community that we live in. 

Integrated Human Practice

Adapt to Change.

CRISPR/Cas13b RNA Base Editor

As we have learnt through our interviews with medical doctors and interaction with the public, it is evident that RNA editing is more promising and better accep-ted in our society. Hence, we reflected on our project and ventured into RNA editing.

Project REPAIR

Aim Even Higher.

Modification-Sensitive Nanopore Sequencing

To address the significant concern around the safety of RNA editing technology, we designed an unbiased, accurate RNA sequencing tool. Expanding the nanopore sequencing platform, we hope to enhance the saftey of RNA editing.

Project Nanopore