Difference between revisions of "Team:Kyoto/Notebook"
| Line 389: | Line 389: | ||
</div> | </div> | ||
<p class="keln_note">*sample5~8 x 2set</p> | <p class="keln_note">*sample5~8 x 2set</p> | ||
| + | <!------------ Table END ------------> | ||
| + | <!-- Table Generated by KELN Author: Tsuzuki --> | ||
| + | <div class="keln_container"> | ||
| + | <a name="0706" class = "kyoto-jump"></a> | ||
| + | <span class="keln_date"><h3>7/6</h3></span> | ||
| + | <span class="keln_exp"><h4>colony_PCR</h4></span> | ||
| + | <span class="keln_researcher"></span> | ||
| + | <table class="keln_table"> | ||
| + | <tr><th>Mangrin colony</th><th>DW (ul)</th><th>Q5 HF2x MasterMix (ul)</th><th>m13 Forward (ul)</th><th>m13 Reverse (ul)</th></tr> | ||
| + | <tr><td>1</td><td>2.0</td><td>2.5</td><td>0.25</td><td>0.25</td></tr> | ||
| + | <tr><td>2</td><td>2.0</td><td>2.5</td><td>0.25</td><td>0.25</td></tr> | ||
| + | <tr><td>3</td><td>2.0</td><td>2.5</td><td>0.25</td><td>0.25</td></tr> | ||
| + | <tr><td>4</td><td>2.0</td><td>2.5</td><td>0.25</td><td>0.25</td></tr> | ||
| + | <tr><td>5</td><td>2.0</td><td>2.5</td><td>0.25</td><td>0.25</td></tr> | ||
| + | </table> | ||
| + | </div> | ||
| + | <p class="keln_note">Store PCR product at 4℃.</p> | ||
<!------------ Table END ------------> | <!------------ Table END ------------> | ||
Revision as of 10:48, 5 September 2018
Notebook
| July | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
| August | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
| September | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
| October | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
7/3
Restriction Enzyme Digestion
Tong, Tamukai| component | volume(µl) |
|---|---|
| BSA | 0.5 |
| buffer(10xH) | 5 |
| EcoR1 | 0.5 |
| Pst1 | 0.5 |
| Dpn1 | 0.5 |
| dH2O | 18 |
add 4µL linearized plasmid backbone (25ng/µL)
add 4µL Enzyme Master Mix
digest 37℃, 30min
add 8µL NaOAc (pH5.2,3M)
add 16µL 99.5% EtOh
Vortex 2sec
add 184µL 99.5% EtOH
add 72µL DW
7/5
OE-PCR
Nambu, Shimazoe| sample | Primer Forward | volume (ul) | Primer Reverse | volume (ul) | template DNA | volume (ul) | Q5 HF 2xMasterMix (ul) | DW (ul) |
|---|---|---|---|---|---|---|---|---|
| 1 | Z007 | 1.25 | Z009 | 1.25 | Yeast genome | 1.0 | 1.0 | 9.0 |
| 2 | Z008 | 1.25 | Z011 | 1.25 | GFP | 1.0 | 1.0 | 9.0 |
| 3 | Z010 | 1.25 | Z012 | 1.25 | Yeast genome | 1.0 | 1.0 | 9.0 |
| 4 | Z007 | 1.25 | Z014 | 1.25 | Yeast genome | 1.0 | 1.0 | 9.0 |
| 5 | Z013 | 1.25 | Z016 | 1.25 | RFP | 1.0 | 1.0 | 9.0 |
| 6 | Z015 | 1.25 | Z012 | 1.25 | Yeast genome | 1.0 | 1.0 | 9.0 |
7/6
OE-PCR
Nambu, Shimazoe| sample | Primer Forward | volume (ul) | Primer Reverse | volume (ul) | Template DNA | volume (ul) | Q5 HF2x Master Mix (ul) | DW (ul) |
|---|---|---|---|---|---|---|---|---|
| 1 | Z007 | 1.25 | Z009 | 1.25 | Yeast genome | 1.0 | 12.5 | 9.0 |
| 2 | Z010 | 1.25 | Z012 | 1.25 | Yeast genome | 1.0 | 12.5 | 9.0 |
| 3 | Z007 | 1.25 | Z014 | 1.25 | Yeast genome | 1.0 | 12.5 | 9.0 |
| 4 | Z015 | 1.25 | Z012 | 1.25 | Yeast genome | 1.0 | 12.5 | 9.0 |
| 5* | Z007 | 1.25 | Z009 | 1.25 | p079 | 1.0 | 12.5 | 9.0 |
| 6* | Z010 | 1.25 | Z012 | 1.25 | p079 | 1.0 | 12.5 | 9.0 |
| 7* | Z007 | 1.25 | Z014 | 1.25 | p079 | 1.0 | 12.5 | 9.0 |
| 8* | Z015 | 1.25 | Z012 | 1.25 | p079 | 1.0 | 12.5 | 9.0 |
*sample5~8 x 2set
7/6
colony_PCR
| Mangrin colony | DW (ul) | Q5 HF2x MasterMix (ul) | m13 Forward (ul) | m13 Reverse (ul) |
|---|---|---|---|---|
| 1 | 2.0 | 2.5 | 0.25 | 0.25 |
| 2 | 2.0 | 2.5 | 0.25 | 0.25 |
| 3 | 2.0 | 2.5 | 0.25 | 0.25 |
| 4 | 2.0 | 2.5 | 0.25 | 0.25 |
| 5 | 2.0 | 2.5 | 0.25 | 0.25 |
Store PCR product at 4℃.