Difference between revisions of "Team:OUC-China/Attributions"

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    <a href="https://2018.igem.org/Team:OUC-China/Description">Oveview</a>
 
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    <a href="https://2018.igem.org/Team:OUC-China/Team">Member</a>
 
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    <a href="https://2018.igem.org/Team:OUC-China/Collaborations">Collaboration</a>
 
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    <a href="https://2018.igem.org/Team:OUC-China/Attributions">Attribution</a>
 
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    <h2 class="hero_header">ATTRIBUTIONS </h2>
 
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      <h1 class="stats">MEMBER'S ATTRIBUTION</h1>
 
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        <p>    From the beginning of 2018, OUC-China have brand new members for this competition season. With the help of experienced team leaders before, we build a team consists of 8 undergraduate students from different majors and two grades. And by sharing happiness and woe this season, now we become close friends and have the tacit understanding. All the members in 2018 OUC-China devoted the best of themselves to develop the project. At the same time, we would have go nowhere without the help from many other people from our school, other institutions and the society. We sincerely appreciate all the help!
 
  
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<br>Yunqian Zhang, captain of OUC-China iGEM 2018 and general affair supervisor, was responsible for designing our project and experiment. She designed and checked all the circuit as well as the primers used in PCR and part standardization. She provided basic experimental scheme for lab administrator. Also she arranged journals for all people to CCiC and Boston.
 
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<br>Yanju Chen, vice-captain of our team, pushed the proceeding of human practice and communication with other teams. She keep connection with all the professors who helped us. She is also responsible for submitting parts and document the experimental characterization. Also she arranged journals for all people to Beijing.
 
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<br>Jiacheng Huang, vice-captain of our team. He selected all the csy4 mutants and hairpin mutants in order to achieve our goal. He was in charge of modeling and excavated deeply in theory who also is one of wiki designer.
 
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<br>Qinbo Qiao, lab administrator and arranged the journal to get visa. Her major work was part standardization and extracted a mount of DNA to make sure the development of experiment.
 
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<br>Xudong Liu, lab administrator, was in charge of construct circuits by using 3A assembly and detected the expression of proteins. Also he was responsible for the measurement of the growth rate of E.coli, providing experimental parameters for model. He changed our experimental scheme with need at any moment.
 
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<br>Qingjie Li, the financial manager of our team and designer for our Video Game——Csy4 Go! She helped us with a lot of biological order for biotechnology companies.
 
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<br>Kai Sun, the web designer and art designer who established the structure of our wiki, poster, team uniform and many other designing works. At the same time, he also take the task of Interlab measurement and primers design.
 
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Anyi Li, was responsible for primers design and plan for human practice.</p>
 
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  <h1 class="stats">MEMBER'S ATTRIBUTION</h1>
 
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<p>  Every member in 2017 OUC-China iGEM gave us practical suggestions.
 
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Yang Liu, the Captain of OUC-China 2011 and Li Kang, the Captain of OUC-China 2012 helped improve the synthetic conception of our project. 
 
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<br>Our PI Prof. Chenguang Liu and Prof. Xingguo Liang offered us constructive advice over our project and gave us long term technical consulting support through the whole procedure.
 
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<br>Our PI Prof.Xingguo Liang offered us experimental lab.
 
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<br>Our PI Prof.Zhe Chi helped us with gene synthesis and financial problems, at the same time, he also provided us with unique advice about project.
 
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<br>Prof. Xiaohua Zhang, from College of Marine Life, Ocean University of China, provided us instrument (Thermo Scientific NanoDrop) to measure the concentration of nucleic acid. </p>
 
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<li><a href="https://2018.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<h2 class="major"><span>Abstract</span></h2>
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<p align="justify">This year, we design a toolkit focused on post-transcriptional regulation, which is composed of a RNA endoribonuclease (Csy4) and a RNA module named miniToe. Csy4 (Csy6f), a member of CRISPR family, recognizes a specific 22nt hairpin. The RNA module was constructed by inserting the 22nt Csy4 recognition site between a RBS and cis-repressive RNA element, which can be specifically cleaved upon Csy4 expression, so the RBS is usually masked. Cleaved at the specific recognition site, it can release the masked RBS, thus endowing the programming of gene expression in the translation level with higher feasibility. We want to use one system to achieve diverse expression of target gene. So we further design four Csy4 mutants and five miniToe mutants. The whole system including five Csy4s and six miniToes is called miniToe family. By combining each Csy4 and hairpin, we can achieve different expression level of the target proteins in a polycistron.</p>
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Revision as of 13:23, 17 September 2018

Team OUC-China: Main

Abstract

This year, we design a toolkit focused on post-transcriptional regulation, which is composed of a RNA endoribonuclease (Csy4) and a RNA module named miniToe. Csy4 (Csy6f), a member of CRISPR family, recognizes a specific 22nt hairpin. The RNA module was constructed by inserting the 22nt Csy4 recognition site between a RBS and cis-repressive RNA element, which can be specifically cleaved upon Csy4 expression, so the RBS is usually masked. Cleaved at the specific recognition site, it can release the masked RBS, thus endowing the programming of gene expression in the translation level with higher feasibility. We want to use one system to achieve diverse expression of target gene. So we further design four Csy4 mutants and five miniToe mutants. The whole system including five Csy4s and six miniToes is called miniToe family. By combining each Csy4 and hairpin, we can achieve different expression level of the target proteins in a polycistron.

Team Members

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