Difference between revisions of "Team:Munich/thisisatest.html"
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| − | <h3>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</ | + | <h3>5/18</h3> |
| + | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | ||
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| − | <h3>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</ | + | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> |
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| − | <h3>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</ | + | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> |
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| − | <h3>DNA preparation for pRED/ET Engineering</ | + | <h4>DNA preparation for pRED/ET Engineering</h4> |
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Revision as of 15:34, 14 September 2018
5/18
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies |
5/24
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/25
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/26
DNA preparation for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
| Results: | pRED/ET: 37,5 ng/ul
pNPTS138-R6KT: 60ng/ul |