Line 47: | Line 47: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <h3>6/4</h3> | ||
+ | <h4>Digestion of Chi6 with XbaI, SpeI</h4> | ||
+ | <table class="table table-borderless"> | ||
+ | <tr> | ||
+ | |||
+ | <td>Participants:</td> | ||
+ | <td>Dominic</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocol:</td> | ||
+ | <td>Ligation</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Notes:</td> | ||
+ | <td> XbaI, SpeI | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Results:</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | <h3>6/4</h3> | ||
+ | <h4>Ligation of Chi6 with pSB1C3</h4> | ||
+ | <table class="table table-borderless"> | ||
+ | <tr> | ||
+ | |||
+ | <td>Participants:</td> | ||
+ | <td>Dominic</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocol:</td> | ||
+ | <td>Ligation</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Notes:</td> | ||
+ | <td> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Results:</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | <h3>6/4</h3> | ||
+ | <h4>Transforming DH5a with pSB1C3_Chi6</h4> | ||
+ | <table class="table table-borderless"> | ||
+ | <tr> | ||
+ | |||
+ | <td>Participants:</td> | ||
+ | <td>Dominic</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocol:</td> | ||
+ | <td>chem trafo</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Notes:</td> | ||
+ | <td> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Results:</td> | ||
+ | <td>no colonies</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
</div> | </div> |
Revision as of 17:27, 14 September 2018
Contents
6/1
Forming oligodimers from single-strand DNA chi6 sequences
Participants: | Dominic |
Protocol: | Oligodimerization |
Notes: | |
Results: |
6/4
PCR to assemble Chi6(double) and Chi6-Mtq2-Chi6
Participants: | Dominic |
Protocol: | PCR |
Notes: | |
Results: |
6/4
Digestion of Chi6 with XbaI, SpeI
Participants: | Dominic |
Protocol: | Ligation |
Notes: | XbaI, SpeI |
Results: |
6/4
Ligation of Chi6 with pSB1C3
Participants: | Dominic |
Protocol: | Ligation |
Notes: | |
Results: |
6/4
Transforming DH5a with pSB1C3_Chi6
Participants: | Dominic |
Protocol: | chem trafo |
Notes: | |
Results: | no colonies |