Difference between revisions of "Team:TUDelft/Wetlab/Protocols"

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<li>Incubate at 90 °C for 10 min. <br>
 
<li>Incubate at 90 °C for 10 min. <br>
 
NOTE: Instead of separate 'cooking' of the cells before the PCR, this step can be incorporated in the PCR program. The initial denaturation step at 98 °C should then be prolonged to 5 minutes (300 seconds). </li>
 
NOTE: Instead of separate 'cooking' of the cells before the PCR, this step can be incorporated in the PCR program. The initial denaturation step at 98 °C should then be prolonged to 5 minutes (300 seconds). </li>
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<li>Prepare the GoTaq master mix for all the samples in a single 1.5 mL tube (NOTE: mind pipetting errors so prepare at little bit more master mix!). For one sample: </li>
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<li>Prepare the GoTaq master mix for all the samples in a single 1.5 mL tube <br>
 +
NOTE: mind pipetting errors so prepare at little bit more master mix!. ><br>
 +
For one sample: </li>
  
 
</ol>
 
</ol>

Revision as of 08:00, 15 September 2018

Wetlab Protocols

Text to write to introduce the protocols


  1. Under sterile conditions, pick a colony and dilute it in 10 µL of milli-Q water.
    NOTE: After you pick the colony, it cannot be used again. It is therefore recommended to make a 'back-up'-plate where you grow the colonies again. This plate should be incubated overnight at 37 °C.
  2. Incubate at 90 °C for 10 min.
    NOTE: Instead of separate 'cooking' of the cells before the PCR, this step can be incorporated in the PCR program. The initial denaturation step at 98 °C should then be prolonged to 5 minutes (300 seconds).
  3. Prepare the GoTaq master mix for all the samples in a single 1.5 mL tube
    NOTE: mind pipetting errors so prepare at little bit more master mix!. >
    For one sample: