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Revision as of 06:16, 17 September 2018
Description
Introduction
The Impacts of Excess Fluoride:
![](https://static.igem.org/mediawiki/2017/8/8a/T--East_Chapel_Hill--design-f1.png)
Source: http://www.bgs.ac.uk/research/groundwater/health/fluoride.html
Solution
What is a Riboswitch?
![](https://static.igem.org/mediawiki/2017/e/ea/T--East_Chapel_Hill--project.png)
2015 Exeter iGEM Team, RNA Riboswitches
![](https://static.igem.org/mediawiki/2017/e/e5/ T--East_Chapel_Hill--design-f3.png)
Aiming Ren, Kanagalaghatta R. Rajashankar, Dinshaw J. Patel “Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch” 2012 Nature 486, 85–89
![](https://static.igem.org/mediawiki/2017/5/50/ T--East_Chapel_Hill--design-f4.png)
Randy B. Stockbridge, Ludmila Kolmakova-Partensky, Tania Shane, Akiko Koide, Shohei Koide, Christopher Miller & Simon Newstead "Crystal structures of a double-barrelled fluoride ion channel." 2015 Nature 525, 548-51
Our Design
![](https://static.igem.org/mediawiki/2017/e/ef/T--East_Chapel_Hill--design-f5.png)
How CHOP works:
- Using the ΔcrcB E. coli strain, which can accumulate fluoride intracellularly
- The Riboswitch detects fluoride
- Fluoride activates the chloramphenicol acetyltransferase enzyme
- Which allows for the growth of bacteria on agar plates with the antibiotic chloramphenicol
References
- Using the ΔcrcB E. coli strain, which can accumulate fluoride intracellularly
- The Riboswitch detects fluoride
- Fluoride activates the chloramphenicol acetyltransferase enzyme
- Which allows for the growth of bacteria on agar plates with the antibiotic chloramphenicol