Project Description

ADaPtat1on : Expanding Toolkit for Acetinobacter baylyi

Acinetobacter baylyi is a gram-negative, soil-dwelling, non-pathogenic, naturally competent and nutritionally versatile organism, known especially for its ability to degrade aromatic compounds. However, only a few tools are available for gene manipulation in Synthetic Biology experiments. This year, we plan to expand this toolkit for A. baylyi ADP1 by making synthetic promoter library along with codon optimized fluorescent reporter proteins for A. baylyi to achieve better control over expression rates. Codon usage table was also created by us using the genomic data. This can potentially open up various new exciting synthetic biology opportunities with this unexplored organism.

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Acinetobacter baylyi is a gram-negative, soil-dwelling, non-pathogenic, naturally competent and nutritionally versatile organism, known especially for its ability to degrade aromatic compounds. However, only a few tools are available for gene manipulation in Synthetic Biology experiments. This year, we plan to expand this toolkit for A. baylyi ADP1 by making synthetic promoter library along with codon optimized fluorescent reporter proteins for A. baylyi to achieve better control over expression rates. Codon usage table was also created by us using the genomic data. This can potentially open up various new exciting synthetic biology opportunities with this unexplored organism.