Samdong2017 (Talk | contribs) |
Samdong2017 (Talk | contribs) |
||
Line 1: | Line 1: | ||
− | + | <html lang="en" dir="ltr" class="client-nojs"> | |
<body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_Worldshaper-XSHS skin-igem action-view"> | <body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_Worldshaper-XSHS skin-igem action-view"> | ||
Line 9: | Line 9: | ||
− | + | <!-- Content div contains HQ_page for HQ styles, Logo and title div, and USER CONTENT --> | |
<div id="content" class="mw-body" role="main"> | <div id="content" class="mw-body" role="main"> | ||
Line 229: | Line 229: | ||
</div> | </div> | ||
+ | </html> |
Revision as of 14:42, 26 September 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract