Difference between revisions of "Team:Munich/chibiobrick1.html"
| Line 124: | Line 124: | ||
<td>Results:</td> | <td>Results:</td> | ||
<td></td> | <td></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <h3>6/26</h3> | ||
| + | <h4>Test PCR for pSB1C3_Chi6</h4> | ||
| + | <table class="table table-borderless"> | ||
| + | <tr> | ||
| + | |||
| + | <td>Participants:</td> | ||
| + | <td>Dominic</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Protocol:</td> | ||
| + | <td>PCR, Agarose gel</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Results:</td> | ||
| + | <td>No bands on the gel. colonies on the plate resulted from contamination</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
Revision as of 12:23, 27 September 2018
6/4
Digestion of Chi6 with XbaI, SpeI
| Participants: | Dominic |
| Protocol: | restriction digest |
| Notes: | XbaI, SpeI |
6/4
Ligation of Chi6 with pSB1C3
| Participants: | Dominic |
| Protocol: | Ligation |
6/4
Transforming DH5a with pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | chem trafo |
| Notes: | |
| Results: | colonies |
6/5
DNA Mini preparation from E. Coli Dh5a pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | |
| Results: | concentration |
6/5
Sequence pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | meh |
| Notes: | |
| Results: | no sequence for the given primers: contamination |
6/6
Redo assembly of Chi6 using A3 assembly
| Participants: | Dominic |
| Protocol: | restriction digest, PCR purification |
| Notes: | XbaI, SpeI |
| Results: |
6/26
Test PCR for pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | PCR, Agarose gel |
| Notes: | |
| Results: | No bands on the gel. colonies on the plate resulted from contamination |