Difference between revisions of "Team:Munich/chibiobrick1.html"

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<html>
 
<div class="event-info">
 
<div class="event-info">
  
 
<h3>6/4</h3>
 
<h3>6/4</h3>
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<h4>Digestion of Chi6 with XbaI, SpeI</h4>
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<h4>Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
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       <td>Protocol:</td>
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       <td>Protocols:</td>
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       <td>restriction digest</td>
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       <td>restriction digest, Ligation, chem trafo, mini-prep, sequencing</td>
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    </tr>
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−
    <tr>
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      <td>Notes:</td>
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      <td> XbaI, SpeI
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−
</td>
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−
    </tr>
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</table>
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−
 
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<h3>6/4</h3>
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<h4>Ligation of Chi6 with pSB1C3</h4>
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<table class="table table-borderless">
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−
    <tr>
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      <td>Participants:</td>
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      <td>Dominic</td>
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    </tr>
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    <tr>
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      <td>Protocol:</td>
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      <td>Ligation</td>
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−
    </tr>
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</table>
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−
 
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<h3>6/4</h3>
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<h4>Transforming DH5a with pSB1C3_Chi6</h4>
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<table class="table table-borderless">
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−
    <tr>
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−
 
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      <td>Participants:</td>
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      <td>Dominic</td>
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−
    </tr>
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−
    <tr>
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      <td>Protocol:</td>
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−
      <td>chem trafo</td>
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−
    </tr>
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    <tr>
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      <td>Notes:</td>
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−
      <td>
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−
</td>
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    </tr>
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<tr>
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      <td>Results:</td>
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      <td>colonies</td>
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    </tr>
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</table>
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<h3>6/5</h3>
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<h4>DNA Mini preparation from E. Coli Dh5a pSB1C3_Chi6</h4>
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<table class="table table-borderless">
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−
    <tr>
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−
 
+
−
      <td>Participants:</td>
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−
      <td>Dominic</td>
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−
    </tr>
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−
    <tr>
+
−
      <td>Protocol:</td>
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−
      <td>mini prep</td>
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−
    </tr>
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−
    <tr>
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−
      <td>Notes:</td>
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−
      <td>
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−
</td>
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−
    </tr>
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−
<tr>
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      <td>Results:</td>
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      <td>concentration</td>
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−
    </tr>
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−
</table>
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−
 
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−
<h3>6/5</h3>
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<h4>Sequence pSB1C3_Chi6</h4>
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−
<table class="table table-borderless">
+
−
    <tr>
+
−
 
+
−
      <td>Participants:</td>
+
−
      <td>Dominic</td>
+
−
    </tr>
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−
    <tr>
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−
      <td>Protocol:</td>
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      <td>meh</td>
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−
    </tr>
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−
    <tr>
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−
      <td>Notes:</td>
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−
      <td>
+
−
</td>
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−
    </tr>
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−
<tr>
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−
      <td>Results:</td>
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−
      <td>no sequence for the given primers: contamination</td>
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−
    </tr>
+
−
</table>
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−
 
+
−
<h3>6/6</h3>
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−
<h4>Redo assembly of Chi6 using A3 assembly</h4>
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−
<table class="table table-borderless">
+
−
    <tr>
+
−
 
+
−
      <td>Participants:</td>
+
−
      <td>Dominic</td>
+
−
    </tr>
+
−
    <tr>
+
−
      <td>Protocol:</td>
+
−
      <td>restriction digest, PCR purification</td>
+
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td> XbaI, SpeI
+
       <td> digest with XbaI, SpeI <br>
 +
transformation of Dh5a
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
 
       <td>Results:</td>
 
       <td>Results:</td>
−
       <td></td>
+
       <td>no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
−
<h3>6/26</h3>
+
<h3>6/6 - 6/26</h3>
−
<h4>Test PCR for pSB1C3_Chi6</h4>
+
<h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
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     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR, Agarose gel</td>
+
       <td>restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td>
+
       <td> digest with XbaI, SpeI <br>
 +
Additionally, samples were purified after the restriction digest <br>
 +
transformation of Dh5a <br>
 +
test pcr was performed with VF2 and VR primers
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
 
       <td>Results:</td>
 
       <td>Results:</td>
−
       <td>No bands on the gel. colonies on the plate resulted from contamination</td>
+
       <td>No bands on the Gel. Colonies on the plate resulted from contamination. We decided to postpone the experiment because everyone in the lab was having difficulities with cloning.</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
 
</div>
 
</div>
 +
</html>

Revision as of 10:07, 29 September 2018

6/4

Assembling pSB1C3_Chi6 with A3 Assembly

Participants: Dominic
Protocols: restriction digest, Ligation, chem trafo, mini-prep, sequencing
Notes: digest with XbaI, SpeI
transformation of Dh5a
Results: no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.

6/6 - 6/26

Redo: Assembling pSB1C3_Chi6 with A3 Assembly

Participants: Dominic
Protocol: restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel
Notes: digest with XbaI, SpeI
Additionally, samples were purified after the restriction digest
transformation of Dh5a
test pcr was performed with VF2 and VR primers
Results: No bands on the Gel. Colonies on the plate resulted from contamination. We decided to postpone the experiment because everyone in the lab was having difficulities with cloning.