Line 89: | Line 89: | ||
</table> | </table> | ||
+ | <h3>8/28</h3> | ||
+ | <h4>growth of RecB, RecD, RecBCD, msbb deficient E.Coli strains</h4> | ||
+ | <table class="table table-borderless"> | ||
+ | <tr> | ||
+ | |||
+ | <td>Participants:</td> | ||
+ | <td>katja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Protocol:</td> | ||
+ | <td>Measuring optical density</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Notes:</td> | ||
+ | <td> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Results:</td> | ||
+ | <td>PIC</td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> |
Revision as of 15:49, 28 September 2018
8/28
growth of msbB and Recombinase deficient E.Coli mutant strains
Participants: | katja |
Protocol: | Measuring optical density |
Notes: | |
Results: | PIC |
8/31
preparing electro-competent cells of WT RecBCD und dRecB
Participants: | katja |
Protocol: | preparing electro-competent cells |
Results: | cells are still viable after preparation, dRecB grows significantly slower than others |
9/3
Transforming E.Coli dRecB with pSB1C3_RecBCD(WT)
Participants: | katja |
Protocol: | epo trafo |
Notes: | |
Results: | colonies |
9/4
DNA midi prep of RecBCD(WT) plasmid from Dh5a
Participants: | katja |
Protocol: | DNA Midi prep |
Notes: | |
Results: | concentration |
8/28
growth of RecB, RecD, RecBCD, msbb deficient E.Coli strains
Participants: | katja |
Protocol: | Measuring optical density |
Notes: | |
Results: | PIC |