Difference between revisions of "Team:Munich/engineering1.html"
| Line 46: | Line 46: | ||
</table> | </table> | ||
| − | |||
<h4>Redo:Transforming E. Coli Rosetta for pRED/ET engineering</h4> | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET engineering</h4> | ||
| + | <em>2018/06/12</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
Revision as of 14:19, 29 September 2018
Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/04
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/08
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. We decided to redo the experiment by electroporation. |
Redo:Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/12
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. pRED/ET engineering seems to not work in Rosetta. we postponed this experiment to do it with PD Dr. Lassak at the LMU again. |