Difference between revisions of "Team:Munich/preparingge.html"

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       <td>Participants:</td>
 
       <td>Participants:</td>
       <td>Dominic</td>
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       <td>Dominic Schwarz</td>
 
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     </tr>
 
     <tr>
 
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       <td>Protocol:</td>
 
       <td>Protocol:</td>
       <td>epo. Trafo</td>
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       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">ELectrocompetent transformation</a>
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</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>

Revision as of 20:31, 15 October 2018

Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering

2018/08/28

Participants: Dominic Schwarz
Protocol:

<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">ELectrocompetent transformation</a>

Notes: inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.
Results: ~200 colonies