Difference between revisions of "Team:Munich/recbiobricks.html"

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</table>
 
</table>
  
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<h3>9/3</h3>
 
 
<h4>Removing PstI restriction site from pSB1C3_RecB</h4>
 
<h4>Removing PstI restriction site from pSB1C3_RecB</h4>
 
<em>2018/09/03</em>
 
<em>2018/09/03</em>

Revision as of 18:18, 29 September 2018

Assembling biobrick for RecB, C, D

2018/08/31

Participants: Enikö
Protocol: PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep
Notes: RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb)

RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb)
RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb)
AT 68° ET 70 s

Results: PIC only one worked, but somehow all were ligated :D stuff missing?

TestPCR for Biobricks of RecB, C, D

2018/09/03

Participants: Enikö
Protocol: PCR, agarose gel
Notes: Primers: VF2 & VR
Results: ?

Removing PstI restriction site from pSB1C3_RecB

2018/09/03

Participants: Enikö
Protocol: PCR, PCR purification, Ligation, gibson assembly, trafo
Notes: because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel

PCR: RecB_BB_PstI-remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD_BB_PstI_remove_lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD-TEV_BB_PstI_remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecB_BB_PstI-remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD_BB_PstI_remove_GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD-TEV_BB_PstI_remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min

Results: ?