Difference between revisions of "Team:Munich/recbiobricks.html"

Revision as of 23:27, 15 October 2018

Assembling biobrick for RecB, C, D

2018/08/31

Participants:	Enikö Baligács
Protocol:	<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>, <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>, <a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini Prep</a>
Notes:	RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb) RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb) RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb) AT 68° ET 70 s
Results:	PIC only one worked, but somehow all were ligated :D stuff missing?

TestPCR for Biobricks of RecB, C, D

2018/09/03

Participants:	Enikö Baligács
Protocol:	<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>
Notes:	Primers: VF2 & VR
Results:	?

Removing PstI restriction site from pSB1C3_RecB

2018/09/03

Participants:	Enikö Baligács
Protocol:	<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://international.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" target="_blank">PCR purification</a>, <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, <a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson assembly</a>, <a href="https://static.igem.org/mediawiki/2018/f/fc/T--Munich--Transformation01.pdf" target="_blank">Transformation</a>
Notes:	because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel PCR: RecB_BB_PstI-remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD_BB_PstI_remove_lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD-TEV_BB_PstI_remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecB_BB_PstI-remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD_BB_PstI_remove_GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD-TEV_BB_PstI_remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min
Results:	?

@@ Line 7: / Line 7: @@
        <td>Participants:</td>
-       <td>Enikö</td>
+       <td>Enikö Baligács</td>
      </tr>
      <tr>
        <td>Protocol:</td>
-       <td>PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep </td>
+       <td>
+<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
+<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
+<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>,
+<a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>,
+<a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>,
+<a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini Prep</a>
+</td>
      </tr>
      <tr>
@@ Line 33: / Line 40: @@
        <td>Participants:</td>
-       <td>Enikö</td>
+       <td>Enikö Baligács</td>
      </tr>
      <tr>
        <td>Protocol:</td>
-       <td>PCR, agarose gel</td>
+       <td>
+<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
+<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>
+</td>
      </tr>
      <tr>
@@ Line 56: / Line 66: @@
        <td>Participants:</td>
-       <td>Enikö</td>
+       <td>Enikö Baligács</td>
      </tr>
      <tr>
        <td>Protocol:</td>
-       <td>PCR, PCR purification, Ligation, gibson assembly, trafo</td>
+       <td>
+<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
+<a href="https://international.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" target="_blank">PCR purification</a>,
+<a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>,
+<a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson assembly</a>,
+<a href="https://static.igem.org/mediawiki/2018/f/fc/T--Munich--Transformation01.pdf" target="_blank">Transformation</a>
+</td>
      </tr>
      <tr>