Difference between revisions of "Team:OUC-China/Safety"

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<br />&emsp;&emspSafety is the acutest problem when it comes to genetically modified organism. We figured this problem out from three aspects: Project design, Lab work and Shipment.
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<br />&emsp;&emsp;Safety is the acutest problem when it comes to genetically modified organism. We figured this problem out from three aspects: Project design, Lab work and Shipment.
 
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<h3>Safe lab work</h3>
 
<h3>Safe lab work</h3>
 
<br />&emsp;&emsp;We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic and abiotic hazards.
 
<br />&emsp;&emsp;We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic and abiotic hazards.
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<div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0d/T--OUC-China--safety001.jpg"  ></div>
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<div align="center"><p style="font-size:80%;color:gray"">Fig.1 Our laboratory</p></div>
 
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  <div align="center"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--OUC-China--Safety01.jpg"  ></div>  
 
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  <div align="center"><p style="font-size:80%;color:gray"">Fig.1 Our personal safety precautions</p></div>  
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  <div align="center"><p style="font-size:80%;color:gray"">Fig.2 Our personal safety precautions</p></div>  
  
 
&emsp;&emsp;Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.
 
&emsp;&emsp;Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.
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<div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c2/T--OUC-China--safety002.jpg"  ></div>
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<div align="center"><p style="font-size:80%;color:gray"">Fig.3 Table for routine inspection</p></div>
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Revision as of 10:45, 4 October 2018

Team OUC-China: Main
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Safety


  Safety is the acutest problem when it comes to genetically modified organism. We figured this problem out from three aspects: Project design, Lab work and Shipment.

Project design


  Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5α is employed as the main chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when using hairpins and csy4 enzyme to tune the expression of genes, it is relatively safe for industrialized applications and scientific research. Based on this, We designed more variants of csy4 enzymes and hairpins. (See more details in xxx and xxx about the variants of csy4 enzymes and hairpins.）So we used these to create a toolkit named miniToe family for synthetic biology which can control the expression of the target gene gradient. What’s more, we used the motA gene, one of the genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit. For the sake of environment, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.

Safe lab work


  We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic and abiotic hazards.


Fig.1 Our laboratory




Fig.2 Our personal safety precautions

  Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.


Fig.3 Table for routine inspection



  
  
  
  
  
  

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