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− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
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− | <h1 class="title"> | + | <h1 class="title">Assessing CooA Production</h1> |
− | <h3 class="date">June | + | <h3 class="date">June 26</h3> |
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− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description">To start testing the production of CooA, we firstly resuspended and transformed the BioBricks from the kit witch we wanted to use (Table 1). This has been done using the iGEM Kit plate instructions (Protocol 1, step 1 - 3) and the NEB 10 b�ta transformation protocol adapted New England Biolabs (Protocol 2), using bought cells with a reported transformation efficienty of 1-3 10^9 cfu/�g pUC19. Inucbation was at 37�C.<br> |
+ | <br> | ||
+ | Table 1 : Resuspended BioBricks from the iGEM 2018 Distribution kit and their uses. | ||
+ | <table border="1" cellpadding="1" cellspacing="1" style="width: 500px;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_K592009</td> | ||
+ | <td style="text-align: center; width: 365px;">Blue Chromoprotein AmilCP</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_B0031</td> | ||
+ | <td style="text-align: center; width: 365px;">Ribosomal binding site (strong), derived from BBa_B0030</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_B0032</td> | ||
+ | <td style="text-align: center; width: 365px;">Ribosomal binding site (medium), derived from BBa_B0030</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_B0030</td> | ||
+ | <td style="text-align: center; width: 365px;">Ribosomal binding site (weak)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_B0015</td> | ||
+ | <td style="text-align: center; width: 365px;">Double terminator (BBa_B0010 & BBa_B0012)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_J23100</td> | ||
+ | <td style="text-align: center; width: 365px;">Strong Constitutive Anderson Promotor</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_J23105</td> | ||
+ | <td style="text-align: center; width: 365px;">Medium Constitutive Anderson Promotor</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_J23113</td> | ||
+ | <td style="text-align: center; width: 365px;">Weak Constitutive Anderson Promotor</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_J45199</td> | ||
+ | <td style="text-align: center; width: 365px;">Banana odor enzyme (ATF1) generator</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_K1184000</td> | ||
+ | <td style="text-align: center; width: 365px;">Killer Red</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_K352001</td> | ||
+ | <td style="text-align: center; width: 365px;">CooA</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="text-align: center; width: 122px;">BBa_K352011</td> | ||
+ | <td style="text-align: center; width: 365px;">CooA responsive system</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <br> | ||
+ | After checking the plates on the 28th of June, we recieved the following results (Table 2):<br> | ||
+ | <br> | ||
+ | Table 2 : Kolonies found after overnight growth. | ||
+ | <table border="1" cellpadding="1" cellspacing="1" style="width: 500px;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_K592009</td> | ||
+ | <td style="width: 277px; text-align: center;">20 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_B0031</td> | ||
+ | <td style="width: 277px; text-align: center;">4 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_B0032</td> | ||
+ | <td style="width: 277px; text-align: center;">5 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_B0030</td> | ||
+ | <td style="width: 277px; text-align: center;">1 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_B0015</td> | ||
+ | <td style="width: 277px; text-align: center;">6 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_J23100</td> | ||
+ | <td style="width: 277px; text-align: center;">1 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_J23105</td> | ||
+ | <td style="width: 277px; text-align: center;">98 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_J23113</td> | ||
+ | <td style="width: 277px; text-align: center;">19 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_J45199</td> | ||
+ | <td style="width: 277px; text-align: center;">0 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_K1184000</td> | ||
+ | <td style="width: 277px; text-align: center;">4 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_K352001</td> | ||
+ | <td style="width: 277px; text-align: center;">2 colonies</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 210px; text-align: center;">BBa_K352011</td> | ||
+ | <td style="width: 277px; text-align: center;">4 colonies</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
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− | <div class="number"><b> | + | <div class="number"><b>1</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing CooA Production</h3> |
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<label for="switch-23" class="more-info">Show more</label> | <label for="switch-23" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">June | + | <h2 class="date" style="color: white;">June 26</h2> |
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− | <h1 class="title"> | + | <h1 class="title">Testrun [TC]</h1> |
− | <h3 class="date"> | + | <h3 class="date">June 27</h3> |
<label class="back" for="switch-24">×</label> | <label class="back" for="switch-24">×</label> | ||
</div> | </div> | ||
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | ||
− | <div class="description"> | + | <div class="description">A short test run was done with the newly assembled cooler. The test concluded that the temperature controller could cool a water droplet to the point of freezing and heat it back up to its boiling point.</div> |
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− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testrun [TC]</h3> |
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<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
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<label for="switch-24" class="more-info">Show more</label> | <label for="switch-24" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">June 27</h2> |
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<input type="checkbox" id="switch-25" class="switch"></input> | <input type="checkbox" id="switch-25" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-25"> |
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<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing CooA Production</h1> |
− | <h3 class="date"> | + | <h3 class="date">June 29</h3> |
<label class="back" for="switch-25">×</label> | <label class="back" for="switch-25">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>After making a 20 mL overnight culture of our NEB 10 b�ta cells containing our BioBricks, we performed a mini prep (Protocol 3). Using our nanodrop spectrophotometer which gave us the following DNA concentrations:<br> |
+ | </p> | ||
+ | |||
+ | <table border="1" cellpadding="1" cellspacing="1" style="width: 500px;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>BBa_K592009</td> | ||
+ | <td>72,5 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_B0031</td> | ||
+ | <td>118,9 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_B0032</td> | ||
+ | <td>52,5 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_B0030</td> | ||
+ | <td>488,6 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_B0015</td> | ||
+ | <td>53,5 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_J23100</td> | ||
+ | <td>148,3 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_J23105</td> | ||
+ | <td>96,3 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_J23113</td> | ||
+ | <td>151,8 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K118400</td> | ||
+ | <td>140,4 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K352001</td> | ||
+ | <td>77,7 ng/�L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K352011</td> | ||
+ | <td>76,8 ng/�L</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <br> | ||
+ | After assessign our results, in the future we will perform the ethanol carry-over steps.<br> | ||
+ | <br> | ||
+ | Also from the previously mentioned overnight culture 1 mL has been used to make Glycerol Stock (Protocol 4)</div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>2</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing CooA Production</h3> |
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<label for="switch-25" class="more-info">Show more</label> | <label for="switch-25" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">June 29</h2> |
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− | <h1 class="title"> | + | <h1 class="title">Draft decision</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 2</h3> |
<label class="back" for="switch-26">×</label> | <label class="back" for="switch-26">×</label> | ||
</div> | </div> | ||
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | ||
− | <div class="description"> | + | <div class="description">The choice to use a computer PSU as a power supply was made. The choice was made on the grounds, that computer PSU have a standard 12, 5 and 3.3V output. This is beneficial because these are the voltages necessary for the selected components.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>01</b></div> | <div class="number"><b>01</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Draft decision</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-26" class="more-info">Show more</label> | <label for="switch-26" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 2</h2> |
</div> | </div> | ||
Line 1,363: | Line 1,528: | ||
<input type="checkbox" id="switch-27" class="switch"></input> | <input type="checkbox" id="switch-27" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-27"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing CooA Production</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 2</h3> |
<label class="back" for="switch-27">×</label> | <label class="back" for="switch-27">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>Today we performed a digestion, dephosphorilation and ligation (following protocols 4 and 5) of a few promotor and RBS BioBricks. Because of administrative compliations only the variants with J23113 and a RBS could be made.</p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>3</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing CooA Production</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-27" class="more-info">Show more</label> | <label for="switch-27" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 2</h2> |
</div> | </div> | ||
Line 1,411: | Line 1,577: | ||
<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Architecture design [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 5</h3> |
<label class="back" for="switch-28">×</label> | <label class="back" for="switch-28">×</label> | ||
</div> | </div> | ||
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | ||
− | <div class="description"> | + | <div class="description">Changes were made to the architecture design of the temperature controller. Different voltage regulators were selected to fit the newly chosen power supply. To better readability of the architecture design a legend was added.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>03</b></div> | <div class="number"><b>03</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Architecture design [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-28" class="more-info">Show more</label> | <label for="switch-28" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 5</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-29" class="switch"></input> | <input type="checkbox" id="switch-29" class="switch"></input> | ||
− | <div class="card | + | <div class="card hardware-card" id="card-29"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Electrical circuit design [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 8</h3> |
<label class="back" for="switch-29">×</label> | <label class="back" for="switch-29">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> |
− | <div class="description"> | + | <div class="description">Custom footprints were made for multiple components and added to the parts library in the PCB design software.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>01</b></div> | <div class="number"><b>01</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Electrical circuit design [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
<label for="switch-29" class="more-info">Show more</label> | <label for="switch-29" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 8</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-30" class="switch"></input> | <input type="checkbox" id="switch-30" class="switch"></input> | ||
− | <div class="card | + | <div class="card hardware-card" id="card-30"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Electrical circuit design [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 9</h3> |
<label class="back" for="switch-30">×</label> | <label class="back" for="switch-30">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> |
− | <div class="description"> | + | <div class="description">The provisional electronic circuit design for the temperature controller was made.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Electrical circuit design [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
<label for="switch-30" class="more-info">Show more</label> | <label for="switch-30" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 9</h2> |
</div> | </div> | ||
Line 1,534: | Line 1,700: | ||
<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Electrical circuit design [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 11</h3> |
<label class="back" for="switch-31">×</label> | <label class="back" for="switch-31">×</label> | ||
</div> | </div> | ||
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | ||
− | <div class="description"> | + | <div class="description">The electronic circuit design for the temperature controller was finished. Measuring point were added to the electronic circuit design.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Electrical circuit design [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-31" class="more-info">Show more</label> | <label for="switch-31" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 11</h2> |
</div> | </div> | ||
Line 1,575: | Line 1,741: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Stock up</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 11</h3> |
<label class="back" for="switch-32">×</label> | <label class="back" for="switch-32">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div> |
− | <div class="description"> | + | <div class="description">To have some basic biobricks ready, we stocked them by doing a plasmid isolation and freeze the DNA. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Stock up</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-32" class="more-info">Show more</label> | <label for="switch-32" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 11</h2> |
</div> | </div> | ||
Line 1,609: | Line 1,775: | ||
<input type="checkbox" id="switch-33" class="switch"></input> | <input type="checkbox" id="switch-33" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-33"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Basic parts</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 11</h3> |
<label class="back" for="switch-33">×</label> | <label class="back" for="switch-33">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div> |
− | <div class="description"> | + | <div class="description">To start on a basic construct we did a digestion and ligation with the parts we isolated earlier. To prevent original constructs we did a defosphorylation after the digestion. We controlled the digestion by doing a gelelktroforeses. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-33" class="more-info">Show more</label> | <label for="switch-33" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 11</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-34" class="switch"></input> | <input type="checkbox" id="switch-34" class="switch"></input> | ||
− | <div class="card | + | <div class="card hardware-card" id="card-34"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Board layout [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 12</h3> |
<label class="back" for="switch-34">×</label> | <label class="back" for="switch-34">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> |
− | <div class="description"> | + | <div class="description">Started working on the board layout for the PCB of the temperature controller.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Board layout [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
<label for="switch-34" class="more-info">Show more</label> | <label for="switch-34" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 12</h2> |
</div> | </div> | ||
Line 1,698: | Line 1,864: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Basic parts</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 12</h3> |
<label class="back" for="switch-35">×</label> | <label class="back" for="switch-35">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Tom van Dijk | Randall de Waard</div> |
− | <div class="description">We | + | <div class="description">We continued with the ligated DNA by transformating it to Neb10Beta. This was plated onto agarplates and incubated for 1 day. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-35" class="more-info">Show more</label> | <label for="switch-35" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 12</h2> |
</div> | </div> | ||
Line 1,740: | Line 1,906: | ||
</svg> | </svg> | ||
<h1 class="title">Board layout [TC]</h1> | <h1 class="title">Board layout [TC]</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 13</h3> |
<label class="back" for="switch-36">×</label> | <label class="back" for="switch-36">×</label> | ||
</div> | </div> | ||
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> | ||
− | <div class="description"> | + | <div class="description">Further work was done to the board layout for the temperature controller.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Board layout [TC]</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Board layout [TC]</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 1,754: | Line 1,920: | ||
</svg> | </svg> | ||
<label for="switch-36" class="more-info">Show more</label> | <label for="switch-36" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 13</h2> |
</div> | </div> | ||
Line 1,781: | Line 1,947: | ||
</svg> | </svg> | ||
<h1 class="title">Basic parts</h1> | <h1 class="title">Basic parts</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 13</h3> |
<label class="back" for="switch-37">×</label> | <label class="back" for="switch-37">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Elise Grootscholten | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">The results of 7/12/2018 were collected, in action of this we decided to plate some ligated and transferred DNA from 7/12/2018 again. This we did because there wasn't a clear grow of colonies on the plates.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 1,795: | Line 1,961: | ||
</svg> | </svg> | ||
<label for="switch-37" class="more-info">Show more</label> | <label for="switch-37" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 13</h2> |
</div> | </div> | ||
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</svg> | </svg> | ||
<h1 class="title">Blue White screening on paper</h1> | <h1 class="title">Blue White screening on paper</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 13</h3> |
<label class="back" for="switch-38">×</label> | <label class="back" for="switch-38">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Elise Grootscholten | Randall de Waard</div> |
− | <div class="description">We | + | <div class="description">We tried to let bacteria grow on paper, therefore we used our own buisnesscards. We let the buisnesscards absorb some LB-medium and put them inside petridishes. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Blue White screening on paper</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Blue White screening on paper</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 1,836: | Line 2,002: | ||
</svg> | </svg> | ||
<label for="switch-38" class="more-info">Show more</label> | <label for="switch-38" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 13</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-39" class="switch"></input> | <input type="checkbox" id="switch-39" class="switch"></input> | ||
− | <div class="card | + | <div class="card hardware-card" id="card-39"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Board layout [TC]</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 15</h3> |
<label class="back" for="switch-39">×</label> | <label class="back" for="switch-39">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Tom van Dijk | Mike Vrieswijk</div> |
− | <div class="description"> | + | <div class="description">Work has been done on the board layout for the temperature controller. The board layout is now finished.</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Board layout [TC]</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path> |
</svg> | </svg> | ||
<label for="switch-39" class="more-info">Show more</label> | <label for="switch-39" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 15</h2> |
</div> | </div> | ||
Line 1,904: | Line 2,070: | ||
</svg> | </svg> | ||
<h1 class="title">Basic parts</h1> | <h1 class="title">Basic parts</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 16</h3> |
<label class="back" for="switch-40">×</label> | <label class="back" for="switch-40">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">To know if the ligation was succesfull we will controll the DNA, therefor we ented the bacteria's with DNA in LB-medium to grow overnight. We also plated the used bacteria's onto new plates to use for further experiments. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>04</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 1,918: | Line 2,084: | ||
</svg> | </svg> | ||
<label for="switch-40" class="more-info">Show more</label> | <label for="switch-40" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 16</h2> |
</div> | </div> | ||
Line 1,944: | Line 2,110: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Blue White screening on paper</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 16</h3> |
<label class="back" for="switch-41">×</label> | <label class="back" for="switch-41">×</label> | ||
</div> | </div> | ||
− | <div class="attendees">Dustin van der Meulen | + | <div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div> |
− | <div class="description">We did | + | <div class="description">We did a blue white screening on our buisnesscards to see if bacteria had grew on them. We transferred the buisnesscards to new petrydishes and added ITPG and X-gal to the petrydishes. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>02</b></div> | <div class="number"><b>02</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Blue White screening on paper</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-41" class="more-info">Show more</label> | <label for="switch-41" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 16</h2> |
</div> | </div> | ||
Line 1,986: | Line 2,152: | ||
</svg> | </svg> | ||
<h1 class="title">Notebook</h1> | <h1 class="title">Notebook</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 17</h3> |
<label class="back" for="switch-42">×</label> | <label class="back" for="switch-42">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Frontend design for the notebook</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 2,000: | Line 2,166: | ||
</svg> | </svg> | ||
<label for="switch-42" class="more-info">Show more</label> | <label for="switch-42" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 17</h2> |
</div> | </div> | ||
Line 2,027: | Line 2,193: | ||
</svg> | </svg> | ||
<h1 class="title">Basic parts</h1> | <h1 class="title">Basic parts</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 17</h3> |
<label class="back" for="switch-43">×</label> | <label class="back" for="switch-43">×</label> | ||
</div> | </div> | ||
− | <div class="attendees">Dustin van der Meulen | | + | <div class="attendees">Rens Boeser | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">The resulst of 7/16/2018 showed that the DNA didnt ligate as planned. To be able to digest enough DNA we did a plasmid isolation again. We digested this DNA and froze it for further use. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>05</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 2,041: | Line 2,207: | ||
</svg> | </svg> | ||
<label for="switch-43" class="more-info">Show more</label> | <label for="switch-43" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 17</h2> |
</div> | </div> | ||
Line 2,060: | Line 2,226: | ||
<input type="checkbox" id="switch-44" class="switch"></input> | <input type="checkbox" id="switch-44" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-44"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Stock up</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 18</h3> |
<label class="back" for="switch-44">×</label> | <label class="back" for="switch-44">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Mike Vrieswijk | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">We did another round of plasmid isolation for stocking. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Stock up</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-44" class="more-info">Show more</label> | <label for="switch-44" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 18</h2> |
</div> | </div> | ||
Line 2,101: | Line 2,267: | ||
<input type="checkbox" id="switch-45" class="switch"></input> | <input type="checkbox" id="switch-45" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-45"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Notebook</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 19</h3> |
<label class="back" for="switch-45">×</label> | <label class="back" for="switch-45">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Designed a way to follow experiments that take up multiple days and eventually designed a notebook entry page and made it a responsive page</div> |
− | + | ||
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-45" class="more-info">Show more</label> | <label for="switch-45" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 19</h2> |
</div> | </div> | ||
Line 2,151: | Line 2,315: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Basic parts</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 19</h3> |
<label class="back" for="switch-46">×</label> | <label class="back" for="switch-46">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Dustin van der Meulen | Mei Ju Goemans| Randall de Waard </div> |
− | <div class="description"> | + | <div class="description">We digested all DNA we have in stock to test for our basic construct. We tested the DNA by doing a gelelktroforeses. </div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>06</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Basic parts</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-46" class="more-info">Show more</label> | <label for="switch-46" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 19</h2> |
</div> | </div> | ||
Line 2,185: | Line 2,349: | ||
<input type="checkbox" id="switch-47" class="switch"></input> | <input type="checkbox" id="switch-47" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-47"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Notebook</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 21</h3> |
<label class="back" for="switch-47">×</label> | <label class="back" for="switch-47">×</label> | ||
</div> | </div> | ||
− | <div class="attendees">Rens Boeser | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Created a one page notebook. Made the notebook entry page more suited for the entry length</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>04</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-47" class="more-info">Show more</label> | <label for="switch-47" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 21</h2> |
</div> | </div> | ||
Line 2,226: | Line 2,390: | ||
<input type="checkbox" id="switch-48" class="switch"></input> | <input type="checkbox" id="switch-48" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-48"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 23</h3> |
<label class="back" for="switch-48">×</label> | <label class="back" for="switch-48">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>Transformations of the biobricks K133071, K173003 and I13453 are performed. K133071 will produce CO2 if there's pyruvate present and K173003 will produce CO2 if urea is present. I13453 is a promotor which will work if there's arabinose present. <br> |
+ | The biobricks were transformed first into electrocometent cells and later into chemical competent cells from the strain NEb10Beta. After transformations the culture was plated on agar plates with antibiotics. The first transformations didn't work out, but the second did, because then the right competent cells were used. </p> | ||
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>1</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-48" class="more-info">Show more</label> | <label for="switch-48" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 23</h2> |
</div> | </div> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">ATP sensor</h1> |
<h3 class="date">July 24</h3> | <h3 class="date">July 24</h3> | ||
<label class="back" for="switch-49">×</label> | <label class="back" for="switch-49">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">New biobricks, used to make an ATP sensor, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">ATP sensor</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
Line 2,316: | Line 2,481: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Gas output</h1> |
<h3 class="date">July 24</h3> | <h3 class="date">July 24</h3> | ||
<label class="back" for="switch-50">×</label> | <label class="back" for="switch-50">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div> |
− | <div class="description"> | + | <div class="description">New biobricks, which produces gasses, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Gas output</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
Line 2,359: | Line 2,523: | ||
</svg> | </svg> | ||
<h1 class="title">Notebook generator</h1> | <h1 class="title">Notebook generator</h1> | ||
− | <h3 class="date">July | + | <h3 class="date">July 24</h3> |
<label class="back" for="switch-51">×</label> | <label class="back" for="switch-51">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Started working on an automatic notebook creator</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook generator</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook generator</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 2,373: | Line 2,537: | ||
</svg> | </svg> | ||
<label for="switch-51" class="more-info">Show more</label> | <label for="switch-51" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 24</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-52" class="switch"></input> | <input type="checkbox" id="switch-52" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-52"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Transformation</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 24</h3> |
<label class="back" for="switch-52">×</label> | <label class="back" for="switch-52">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>Today we did a transformation with new biobricks. The biobricks were built into chemically competent cells from the strain NEb10Beta. After transformation the culture was plated on agar plates with antibiotics. The results were checked the next day. </p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>1</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Transformation</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-52" class="more-info">Show more</label> | <label for="switch-52" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 24</h2> |
</div> | </div> | ||
Line 2,433: | Line 2,598: | ||
<input type="checkbox" id="switch-53" class="switch"></input> | <input type="checkbox" id="switch-53" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-53"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 24</h3> |
<label class="back" for="switch-53">×</label> | <label class="back" for="switch-53">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>To be able to always get the necessary biobricks, there have been made glycerolstocks of the transformed biobricks K133071, K173003 and I13453. The glycerolstocks are stored at -80 degrees Celsius. If needed, they can be retrieved from this storage to use for experiments. </p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>2</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-53" class="more-info">Show more</label> | <label for="switch-53" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 24</h2> |
</div> | </div> | ||
Line 2,474: | Line 2,640: | ||
<input type="checkbox" id="switch-54" class="switch"></input> | <input type="checkbox" id="switch-54" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-54"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Notebook generator</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 25</h3> |
<label class="back" for="switch-54">×</label> | <label class="back" for="switch-54">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Automatic generation of entries completed</div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook generator</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-54" class="more-info">Show more</label> | <label for="switch-54" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 25</h2> |
</div> | </div> | ||
Line 2,516: | Line 2,681: | ||
<input type="checkbox" id="switch-55" class="switch"></input> | <input type="checkbox" id="switch-55" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-55"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Google Drive API</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 25</h3> |
<label class="back" for="switch-55">×</label> | <label class="back" for="switch-55">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Used the Google drive REST API to download the entries from drive</div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Google Drive API</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-55" class="more-info">Show more</label> | <label for="switch-55" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 25</h2> |
</div> | </div> | ||
Line 2,565: | Line 2,729: | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Automatic uploader</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 25</h3> |
<label class="back" for="switch-56">×</label> | <label class="back" for="switch-56">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Used the igemwiki API to update pages using software</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>01</b></div> | <div class="number"><b>01</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Automatic uploader</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-56" class="more-info">Show more</label> | <label for="switch-56" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 25</h2> |
</div> | </div> | ||
Line 2,599: | Line 2,763: | ||
<input type="checkbox" id="switch-57" class="switch"></input> | <input type="checkbox" id="switch-57" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-57"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Transformation</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 27</h3> |
<label class="back" for="switch-57">×</label> | <label class="back" for="switch-57">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>To be able to always get the necessary biobricks, we made an glycerol stock after every transformation. The glycerol stocks are stored at -80 degrees Celsius. If needed, they can retrieved from this storage to use for experiments. </p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>2</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Transformation</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-57" class="more-info">Show more</label> | <label for="switch-57" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 27</h2> |
</div> | </div> | ||
Line 2,640: | Line 2,805: | ||
<input type="checkbox" id="switch-58" class="switch"></input> | <input type="checkbox" id="switch-58" class="switch"></input> | ||
− | <div class="card | + | <div class="card wetlab-card" id="card-58"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 27</h3> |
<label class="back" for="switch-58">×</label> | <label class="back" for="switch-58">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">UNKNOWN</div> |
− | <div class="description"> | + | <div class="description"><p>For further experiments there is isolated DNA needed of the biobricks J23100, K133071, K173003 and I134353. The DNA is isolated out of the bacteria with the help of a plasmid purifaction kit. After isolation this DNA can be used for digestion and ligation or other experiments. <span style="font-size:12px;">J23100 (from the glycerolstock): 325,59 ng/ul, K173003: 217,06 ng/ul, K133071: 186,79 ng/ul, </span>I13453: 88,18 ng/ul</p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>3</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> |
</svg> | </svg> | ||
<label for="switch-58" class="more-info">Show more</label> | <label for="switch-58" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 27</h2> |
</div> | </div> | ||
Line 2,688: | Line 2,854: | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Automation program</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 29</h3> |
<label class="back" for="switch-59">×</label> | <label class="back" for="switch-59">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Connected the page uploader to the notebook generator</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Automation program</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-59" class="more-info">Show more</label> | <label for="switch-59" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 29</h2> |
</div> | </div> | ||
Line 2,729: | Line 2,895: | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Automatic uploader</h1> |
− | <h3 class="date">July | + | <h3 class="date">July 29</h3> |
<label class="back" for="switch-60">×</label> | <label class="back" for="switch-60">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Created a program that uploads all updated pages</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
<div class="number"><b>02</b></div> | <div class="number"><b>02</b></div> | ||
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Automatic uploader</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-60" class="more-info">Show more</label> | <label for="switch-60" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">July | + | <h2 class="date" style="color: white;">July 29</h2> |
</div> | </div> | ||
Line 2,770: | Line 2,936: | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Page generator</h1> |
− | <h3 class="date"> | + | <h3 class="date">July 31</h3> |
<label class="back" for="switch-61">×</label> | <label class="back" for="switch-61">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Built a page builder that automatically adds the header and footer before uploading</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>01</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Page generator</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-61" class="more-info">Show more</label> | <label for="switch-61" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">July 31</h2> |
</div> | </div> | ||
Line 2,811: | Line 2,977: | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Google Drive API</h1> |
− | <h3 class="date"> | + | <h3 class="date">July 31</h3> |
<label class="back" for="switch-62">×</label> | <label class="back" for="switch-62">×</label> | ||
</div> | </div> | ||
<div class="attendees">Rens Boeser</div> | <div class="attendees">Rens Boeser</div> | ||
− | <div class="description"> | + | <div class="description">Redone the google API handler for reusability and readability and made the google API handler generic</div> |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Google Drive API</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-62" class="more-info">Show more</label> | <label for="switch-62" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">July 31</h2> |
</div> | </div> | ||
Line 2,845: | Line 3,011: | ||
<input type="checkbox" id="switch-63" class="switch"></input> | <input type="checkbox" id="switch-63" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-63"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Automation program</h1> |
− | <h3 class="date"> | + | <h3 class="date">July 31</h3> |
<label class="back" for="switch-63">×</label> | <label class="back" for="switch-63">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">revamped the main program</div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>02</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Automation program</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-63" class="more-info">Show more</label> | <label for="switch-63" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">July 31</h2> |
</div> | </div> | ||
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<input type="checkbox" id="switch-64" class="switch"></input> | <input type="checkbox" id="switch-64" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-64"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Automation program</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 1</h3> |
<label class="back" for="switch-64">×</label> | <label class="back" for="switch-64">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Finished redoing the application for generating and uploading pages</div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>03</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Automation program</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-64" class="more-info">Show more</label> | <label for="switch-64" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 1</h2> |
</div> | </div> | ||
Line 2,929: | Line 3,093: | ||
<input type="checkbox" id="switch-65" class="switch"></input> | <input type="checkbox" id="switch-65" class="switch"></input> | ||
− | <div class="card | + | <div class="card software-card" id="card-65"> |
<div class="entryform"> | <div class="entryform"> | ||
<div class="titlebar"> | <div class="titlebar"> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Notebook</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 2</h3> |
<label class="back" for="switch-65">×</label> | <label class="back" for="switch-65">×</label> | ||
</div> | </div> | ||
− | <div class="attendees"> | + | <div class="attendees">Rens Boeser</div> |
− | <div class="description"> | + | <div class="description">Added month categorization to the page</div> |
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>05</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Notebook</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
− | <path d=" | + | <path d="M14.6,16.6L19.2,12L14.6,7.4L16,6L22,12L16,18L14.6,16.6M9.4,16.6L4.8,12L9.4,7.4L8,6L2,12L8,18L9.4,16.6Z"></path> |
</svg> | </svg> | ||
<label for="switch-65" class="more-info">Show more</label> | <label for="switch-65" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 2</h2> |
</div> | </div> | ||
Line 2,978: | Line 3,141: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Transformation</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 9</h3> |
<label class="back" for="switch-66">×</label> | <label class="back" for="switch-66">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>For further experiments we needed isolated DNA. The DNA is isolated out of the bacteria with the help of a plasmid purifaction kit. After isolation this DNA can be used for digestion and ligation or other experiments.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>3</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Transformation</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-66" class="more-info">Show more</label> | <label for="switch-66" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 9</h2> |
</div> | </div> | ||
Line 3,021: | Line 3,184: | ||
</svg> | </svg> | ||
<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date">August | + | <h3 class="date">August 10</h3> |
<label class="back" for="switch-67">×</label> | <label class="back" for="switch-67">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>The biobricks J23100, K133071, K173003 and I134353 were sucessfully digested after the second time. After the digestions the biobricks K133071 and K173003 were dephosphorylated and ligated with the inserts J23100 and I13453. This was done in the original backbone of K133071 and K173003, and not another control backbone. To know if the biobricks were right ligated this was done by testing practically. See the experiments: <em>Testing gas production.</em> </p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>4</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 3,036: | Line 3,199: | ||
</svg> | </svg> | ||
<label for="switch-67" class="more-info">Show more</label> | <label for="switch-67" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 10</h2> |
</div> | </div> | ||
Line 3,062: | Line 3,225: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Urea and sodium pyruvate test for resistence E.coli</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 10</h3> |
<label class="back" for="switch-68">×</label> | <label class="back" for="switch-68">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"> | + | <div class="description"><p><span style="font-size:14px;">Testing different amounts of urea and sodiumpyruvate to know which concentrations the bacteria survive.</span></p> |
+ | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>1</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Urea and sodium pyruvate test for resistence E.coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-68" class="more-info">Show more</label> | <label for="switch-68" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 10</h2> |
</div> | </div> | ||
Line 3,103: | Line 3,267: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 13</h3> |
<label class="back" for="switch-69">×</label> | <label class="back" for="switch-69">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Transformations of the biobricks (K133071 + J23100), (K13071 + I1345), (K173003 + J23100) and (K173003 + I13453) in NEB10B�ta. There was no grow except for the biobrick combination K133071 + J23100.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>5</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-69" class="more-info">Show more</label> | <label for="switch-69" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 13</h2> |
</div> | </div> | ||
Line 3,145: | Line 3,309: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 13</h3> |
<label class="back" for="switch-70">×</label> | <label class="back" for="switch-70">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>A colony PCR is done for the NEB10B�ta E.coli cells with expected the biobrick combination of K133071 with J23100. Nevertheless, on a gel the difference with and without promotor couldn't be seen. So there must be another way of proving the right biobricks are there.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>6</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-70" class="more-info">Show more</label> | <label for="switch-70" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 13</h2> |
</div> | </div> | ||
Line 3,187: | Line 3,351: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 13</h3> |
<label class="back" for="switch-71">×</label> | <label class="back" for="switch-71">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"> | + | <div class="description">The ligations and transformations of the biobricks K173003 + J23100, K173003 + I13453, and K133071 + I13453 has been performed again. There was a lot of grow on the agar plates. To know if the biobricks were right ligated this was done by testing practically. See the experiments: <em>Testing gas production.</em> </div> |
− | + | ||
− | + | ||
− | </div> | + | |
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>7</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-71" class="more-info">Show more</label> | <label for="switch-71" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 13</h2> |
</div> | </div> | ||
Line 3,232: | Line 3,393: | ||
</svg> | </svg> | ||
<h1 class="title">Testing gas production</h1> | <h1 class="title">Testing gas production</h1> | ||
− | <h3 class="date">August | + | <h3 class="date">August 16</h3> |
<label class="back" for="switch-72">×</label> | <label class="back" for="switch-72">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Making a set up for the gas production testing and testing it with NEB10B�ta with the expected biobricks in it (K133071 + J23100) and a negative control.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>1</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
Line 3,247: | Line 3,408: | ||
</svg> | </svg> | ||
<label for="switch-72" class="more-info">Show more</label> | <label for="switch-72" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 16</h2> |
</div> | </div> | ||
Line 3,273: | Line 3,434: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Testing gas production</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 23</h3> |
<label class="back" for="switch-73">×</label> | <label class="back" for="switch-73">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Making a set up for the gas production testing and testing it with NEB10B�ta with the expected biobricks in it (K133071 + I13453), (K173003 + J23100), (K173003 + I13453) and a negative control.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>2</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-73" class="more-info">Show more</label> | <label for="switch-73" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 23</h2> |
</div> | </div> | ||
Line 3,315: | Line 3,476: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Testing gas production</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 23</h3> |
<label class="back" for="switch-74">×</label> | <label class="back" for="switch-74">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Gasproduction testing for the biobricks (K173003 + I13453), (K133071 + I13453) and negative controls (B0015 and K133071 without urea and arabinose).<br> |
+ | K173003 + I13453 is tested with sodiumpyruvate and arabinose for gasproduction and K133071 + I13453 is tested with urea and arabinose for gasproduction.<br> | ||
+ | The negative control also produces a little bit gas.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>3</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-74" class="more-info">Show more</label> | <label for="switch-74" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 23</h2> |
</div> | </div> | ||
Line 3,357: | Line 3,520: | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Testing gas production</h1> |
− | <h3 class="date">August | + | <h3 class="date">August 23</h3> |
<label class="back" for="switch-75">×</label> | <label class="back" for="switch-75">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p | + | <div class="description"><p>Testing the gasproduction of the colonies 9 and 10 of biobricks (K173003 + J23100), colonies 5 and 7 of biobricks (K173003 + I13453) and colony 4 of biobricks (K133071 + I13453) with and without centrifuging the bacteria. There is also a negative control (J04450 pSB1K3) with Kanamycine. The negative contol started with a lot of gas inside the tube. We can not see wether there is produced more after a day or not. This have to be tested later.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>4</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
<label for="switch-75" class="more-info">Show more</label> | <label for="switch-75" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;">August | + | <h2 class="date" style="color: white;">August 23</h2> |
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date"> | + | <h3 class="date">August 27</h3> |
<label class="back" for="switch-76">×</label> | <label class="back" for="switch-76">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Minipreps are made of colonies 11 and 19 of biobrick combination K133071 + J23100. Results: 11. 270,57 ng/ul 19. 253,59 ng/ul.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>8</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
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− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">August 27</h2> |
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date"> | + | <h3 class="date">August 27</h3> |
<label class="back" for="switch-77">×</label> | <label class="back" for="switch-77">×</label> | ||
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>A miniprep of the biobrick K352002 is made. The concentration is 69,77 ng/ul.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>9</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
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<label for="switch-77" class="more-info">Show more</label> | <label for="switch-77" class="more-info">Show more</label> | ||
− | <h2 class="date" style="color: white;"> | + | <h2 class="date" style="color: white;">August 27</h2> |
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<input type="checkbox" id="switch-78" class="switch"></input> | <input type="checkbox" id="switch-78" class="switch"></input> | ||
<div class="card wetlab-card" id="card-78"> | <div class="card wetlab-card" id="card-78"> | ||
+ | |||
+ | <div class="entryform"> | ||
+ | <div class="titlebar"> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <h1 class="title">Urea and sodium pyruvate test for resistence E.coli</h1> | ||
+ | <h3 class="date">August 28</h3> | ||
+ | <label class="back" for="switch-78">×</label> | ||
+ | </div> | ||
+ | <div class="attendees">UNKNOWN</div> | ||
+ | <div class="description"><p><span style="font-size:14px;">Testing different amounts of urea and sodiumpyruvate to know which concentrations the bacteria survive.</span></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="cardform"> | ||
+ | <div class="number"><b>2</b></div> | ||
+ | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Urea and sodium pyruvate test for resistence E.coli</h3> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <label for="switch-78" class="more-info">Show more</label> | ||
+ | <h2 class="date" style="color: white;">August 28</h2> | ||
+ | </div> | ||
+ | |||
+ | </div><style> | ||
+ | #switch-79:checked ~ #card-79 { | ||
+ | width: 100%; | ||
+ | height: auto; | ||
+ | } | ||
+ | #switch-79:checked ~ #card-79 .entryform { | ||
+ | display: block; | ||
+ | } | ||
+ | #switch-79:checked ~ #card-79 .cardform, | ||
+ | #switch-79:checked ~ #card-79::before, | ||
+ | #switch-79:checked ~ #card-79::after { | ||
+ | display: none; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <input type="checkbox" id="switch-79" class="switch"></input> | ||
+ | <div class="card wetlab-card" id="card-79"> | ||
+ | |||
+ | <div class="entryform"> | ||
+ | <div class="titlebar"> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <h1 class="title">Chemo competent cells </h1> | ||
+ | <h3 class="date">August 30</h3> | ||
+ | <label class="back" for="switch-79">×</label> | ||
+ | </div> | ||
+ | <div class="attendees">UNKNOWN</div> | ||
+ | <div class="description"><p></p></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="cardform"> | ||
+ | <div class="number"><b>1</b></div> | ||
+ | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Chemo competent cells </h3> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <label for="switch-79" class="more-info">Show more</label> | ||
+ | <h2 class="date" style="color: white;">August 30</h2> | ||
+ | </div> | ||
+ | |||
+ | </div><style> | ||
+ | #switch-80:checked ~ #card-80 { | ||
+ | width: 100%; | ||
+ | height: auto; | ||
+ | } | ||
+ | #switch-80:checked ~ #card-80 .entryform { | ||
+ | display: block; | ||
+ | } | ||
+ | #switch-80:checked ~ #card-80 .cardform, | ||
+ | #switch-80:checked ~ #card-80::before, | ||
+ | #switch-80:checked ~ #card-80::after { | ||
+ | display: none; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <input type="checkbox" id="switch-80" class="switch"></input> | ||
+ | <div class="card wetlab-card" id="card-80"> | ||
+ | |||
+ | <div class="entryform"> | ||
+ | <div class="titlebar"> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
+ | <h3 class="date">September 7</h3> | ||
+ | <label class="back" for="switch-80">×</label> | ||
+ | </div> | ||
+ | <div class="attendees">UNKNOWN</div> | ||
+ | <div class="description"><p>Digestions, gelelectrophoresis, dephosphorylations and ligations of different biobrick combinations. These biobrick combinations are 4 different promotors (J23100, I13453, K352002, K352003) with 4 different gasproduction biobricks (k173003, K173013, K133071, K133116). J23100 is about 1 kb to long. The rest seems likely to be right digested. The ligations will be transformed in NEB10b�ta and digested again as control.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="cardform"> | ||
+ | <div class="number"><b>10</b></div> | ||
+ | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <label for="switch-80" class="more-info">Show more</label> | ||
+ | <h2 class="date" style="color: white;">September 7</h2> | ||
+ | </div> | ||
+ | |||
+ | </div><style> | ||
+ | #switch-81:checked ~ #card-81 { | ||
+ | width: 100%; | ||
+ | height: auto; | ||
+ | } | ||
+ | #switch-81:checked ~ #card-81 .entryform { | ||
+ | display: block; | ||
+ | } | ||
+ | #switch-81:checked ~ #card-81 .cardform, | ||
+ | #switch-81:checked ~ #card-81::before, | ||
+ | #switch-81:checked ~ #card-81::after { | ||
+ | display: none; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <input type="checkbox" id="switch-81" class="switch"></input> | ||
+ | <div class="card wetlab-card" id="card-81"> | ||
+ | |||
+ | <div class="entryform"> | ||
+ | <div class="titlebar"> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
+ | <h3 class="date">September 7</h3> | ||
+ | <label class="back" for="switch-81">×</label> | ||
+ | </div> | ||
+ | <div class="attendees">UNKNOWN</div> | ||
+ | <div class="description"><p>Transformations of the biobrick combinations in NEB10b�ta. The transformations are plated on kanamycine agar plates, because all the ligations were done in pSB1K3 (kanamycine resistence) backbone. Pink and with colonies appeared after incubation by 37 degrees Celcius for about 12h. The white colonies will be used for further experiments.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="cardform"> | ||
+ | <div class="number"><b>11</b></div> | ||
+ | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
+ | <svg viewBox="0 0 24 24"> | ||
+ | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
+ | </svg> | ||
+ | <label for="switch-81" class="more-info">Show more</label> | ||
+ | <h2 class="date" style="color: white;">September 7</h2> | ||
+ | </div> | ||
+ | |||
+ | </div><style> | ||
+ | #switch-82:checked ~ #card-82 { | ||
+ | width: 100%; | ||
+ | height: auto; | ||
+ | } | ||
+ | #switch-82:checked ~ #card-82 .entryform { | ||
+ | display: block; | ||
+ | } | ||
+ | #switch-82:checked ~ #card-82 .cardform, | ||
+ | #switch-82:checked ~ #card-82::before, | ||
+ | #switch-82:checked ~ #card-82::after { | ||
+ | display: none; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <input type="checkbox" id="switch-82" class="switch"></input> | ||
+ | <div class="card wetlab-card" id="card-82"> | ||
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
<h3 class="date">September 11</h3> | <h3 class="date">September 11</h3> | ||
− | <label class="back" for="switch- | + | <label class="back" for="switch-82">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-82" class="more-info">Show more</label> |
<h2 class="date" style="color: white;">September 11</h2> | <h2 class="date" style="color: white;">September 11</h2> | ||
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− | <input type="checkbox" id="switch- | + | <input type="checkbox" id="switch-83" class="switch"></input> |
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
<h3 class="date">September 11</h3> | <h3 class="date">September 11</h3> | ||
− | <label class="back" for="switch- | + | <label class="back" for="switch-83">×</label> |
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<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-83" class="more-info">Show more</label> |
<h2 class="date" style="color: white;">September 11</h2> | <h2 class="date" style="color: white;">September 11</h2> | ||
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date">September | + | <h3 class="date">September 13</h3> |
− | <label class="back" for="switch- | + | <label class="back" for="switch-84">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Digestions have been done of the biobrickcombinations K352002 +K133116 + pSB1K3 and K352003 + K133116 + pSB1K3 with restriction-enzymes ScaI and SmaI</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>15</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-84" class="more-info">Show more</label> |
− | <h2 class="date" style="color: white;">September | + | <h2 class="date" style="color: white;">September 13</h2> |
</div> | </div> | ||
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</style> | </style> | ||
− | <input type="checkbox" id="switch- | + | <input type="checkbox" id="switch-85" class="switch"></input> |
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date">September | + | <h3 class="date">September 14</h3> |
− | <label class="back" for="switch- | + | <label class="back" for="switch-85">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>All the digestions of experiment 13 have digested again with SmaI for an our at 25 degrees Celcius. Because earlier the digestions have been put immediately bt 37 degrees Celcius. It looks like H1, J1 and D2 include the right biobricks. </p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>16</b></div> |
<h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> | ||
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-85" class="more-info">Show more</label> |
− | <h2 class="date" style="color: white;">September | + | <h2 class="date" style="color: white;">September 14</h2> |
</div> | </div> | ||
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</style> | </style> | ||
− | <input type="checkbox" id="switch- | + | <input type="checkbox" id="switch-86" class="switch"></input> |
− | <div class="card wetlab-card" id="card- | + | <div class="card wetlab-card" id="card-86"> |
<div class="entryform"> | <div class="entryform"> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> |
<h3 class="date">September 17</h3> | <h3 class="date">September 17</h3> | ||
− | <label class="back" for="switch- | + | <label class="back" for="switch-86">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Minipreps of the biobrick combinations: K352002+K133116+pSB1K3 (1) and K352003+K133116+pSB1K3 (2) are done. The DNA concentration is for 1:189,3 ng/�l and for 2: 355,3 ng/�l.</p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="cardform"> | <div class="cardform"> | ||
− | <div class="number"><b> | + | <div class="number"><b>14</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Assessing Gas Production BioBricks in E.Coli</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-86" class="more-info">Show more</label> |
<h2 class="date" style="color: white;">September 17</h2> | <h2 class="date" style="color: white;">September 17</h2> | ||
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</style> | </style> | ||
− | <input type="checkbox" id="switch- | + | <input type="checkbox" id="switch-87" class="switch"></input> |
− | <div class="card wetlab-card" id="card- | + | <div class="card wetlab-card" id="card-87"> |
<div class="entryform"> | <div class="entryform"> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <h1 class="title"> | + | <h1 class="title">Testing gas production</h1> |
− | <h3 class="date">September | + | <h3 class="date">September 17</h3> |
− | <label class="back" for="switch- | + | <label class="back" for="switch-87">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
− | <div class="description"><p> | + | <div class="description"><p>Different strains of E.coli are tested as negative control. Those strains were: NEB10B�ta, BL21 (DE3), BL21, HB101, DH5alpha and JM109. NEB10B�ta produced the most gas. BL21, HB101 and JM101 produced none/almost none gas. Because BL21 isn't a K12 strain we can not use that one. That is why we will test further gasproduction in HB101 and JM109. And maybe those will be our final E.coli strains.</p> |
</div> | </div> | ||
</div> | </div> | ||
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− | <div class="number"><b> | + | <div class="number"><b>5</b></div> |
− | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;"> | + | <h3 class="title" style="color: white; padding: 10px 5px 5px 5px;">Testing gas production</h3> |
<svg viewBox="0 0 24 24"> | <svg viewBox="0 0 24 24"> | ||
<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
</svg> | </svg> | ||
− | <label for="switch- | + | <label for="switch-87" class="more-info">Show more</label> |
− | <h2 class="date" style="color: white;">September | + | <h2 class="date" style="color: white;">September 17</h2> |
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<h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | <h1 class="title">Assessing Gas Production BioBricks in E.Coli</h1> | ||
− | <h3 class="date">September | + | <h3 class="date">September 20</h3> |
− | <label class="back" for="switch- | + | <label class="back" for="switch-88">×</label> |
</div> | </div> | ||
<div class="attendees">UNKNOWN</div> | <div class="attendees">UNKNOWN</div> | ||
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<path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | <path d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z"></path> | ||
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− | <h2 class="date" style="color: white;">September | + | <h2 class="date" style="color: white;">September 20</h2> |
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Revision as of 18:21, 8 October 2018
Notebook
show hardware entries
show software entries
Team page
May 10
Footer
May 20
Footer
May 21
Notebook
May 24
Header
May 27
Main template
May 28
Header
May 28
Team
May 29
Main page
June 7
Team
June 9
Header
June 9
Header
June 10
Construction pages
June 13
Header
June 14
Main page
June 14
Architecture design [TC]
June 18
Human Practices page
June 22
Main page
June 23
Architecture design [TC]
June 24
First prototype [TC]
June 25
Team
June 26
Second prototype [TC]
June 26
Table 1 : Resuspended BioBricks from the iGEM 2018 Distribution kit and their uses.
BBa_K592009 | Blue Chromoprotein AmilCP |
BBa_B0031 | Ribosomal binding site (strong), derived from BBa_B0030 |
BBa_B0032 | Ribosomal binding site (medium), derived from BBa_B0030 |
BBa_B0030 | Ribosomal binding site (weak) |
BBa_B0015 | Double terminator (BBa_B0010 & BBa_B0012) |
BBa_J23100 | Strong Constitutive Anderson Promotor |
BBa_J23105 | Medium Constitutive Anderson Promotor |
BBa_J23113 | Weak Constitutive Anderson Promotor |
BBa_J45199 | Banana odor enzyme (ATF1) generator |
BBa_K1184000 | Killer Red |
BBa_K352001 | CooA |
BBa_K352011 | CooA responsive system |
After checking the plates on the 28th of June, we recieved the following results (Table 2):
Table 2 : Kolonies found after overnight growth.
BBa_K592009 | 20 colonies |
BBa_B0031 | 4 colonies |
BBa_B0032 | 5 colonies |
BBa_B0030 | 1 colonies |
BBa_B0015 | 6 colonies |
BBa_J23100 | 1 colonies |
BBa_J23105 | 98 colonies |
BBa_J23113 | 19 colonies |
BBa_J45199 | 0 colonies |
BBa_K1184000 | 4 colonies |
BBa_K352001 | 2 colonies |
BBa_K352011 | 4 colonies |
Assessing CooA Production
June 26
Testrun [TC]
June 27
After making a 20 mL overnight culture of our NEB 10 b�ta cells containing our BioBricks, we performed a mini prep (Protocol 3). Using our nanodrop spectrophotometer which gave us the following DNA concentrations:
BBa_K592009 | 72,5 ng/�L |
BBa_B0031 | 118,9 ng/�L |
BBa_B0032 | 52,5 ng/�L |
BBa_B0030 | 488,6 ng/�L |
BBa_B0015 | 53,5 ng/�L |
BBa_J23100 | 148,3 ng/�L |
BBa_J23105 | 96,3 ng/�L |
BBa_J23113 | 151,8 ng/�L |
BBa_K118400 | 140,4 ng/�L |
BBa_K352001 | 77,7 ng/�L |
BBa_K352011 | 76,8 ng/�L |
After assessign our results, in the future we will perform the ethanol carry-over steps.
Also from the previously mentioned overnight culture 1 mL has been used to make Glycerol Stock (Protocol 4)
Assessing CooA Production
June 29
Draft decision
July 2
Today we performed a digestion, dephosphorilation and ligation (following protocols 4 and 5) of a few promotor and RBS BioBricks. Because of administrative compliations only the variants with J23113 and a RBS could be made.
Assessing CooA Production
July 2
Architecture design [TC]
July 5
Electrical circuit design [TC]
July 8
Electrical circuit design [TC]
July 9
Electrical circuit design [TC]
July 11
Stock up
July 11
Basic parts
July 11
Board layout [TC]
July 12
Basic parts
July 12
Board layout [TC]
July 13
Basic parts
July 13
Blue White screening on paper
July 13
Board layout [TC]
July 15
Basic parts
July 16
Blue White screening on paper
July 16
Notebook
July 17
Basic parts
July 17
Stock up
July 18
Notebook
July 19
Basic parts
July 19
Notebook
July 21
Transformations of the biobricks K133071, K173003 and I13453 are performed. K133071 will produce CO2 if there's pyruvate present and K173003 will produce CO2 if urea is present. I13453 is a promotor which will work if there's arabinose present.
The biobricks were transformed first into electrocometent cells and later into chemical competent cells from the strain NEb10Beta. After transformations the culture was plated on agar plates with antibiotics. The first transformations didn't work out, but the second did, because then the right competent cells were used.
Assessing Gas Production BioBricks in E.Coli
July 23
ATP sensor
July 24
Gas output
July 24
Notebook generator
July 24
Today we did a transformation with new biobricks. The biobricks were built into chemically competent cells from the strain NEb10Beta. After transformation the culture was plated on agar plates with antibiotics. The results were checked the next day.
Transformation
July 24
To be able to always get the necessary biobricks, there have been made glycerolstocks of the transformed biobricks K133071, K173003 and I13453. The glycerolstocks are stored at -80 degrees Celsius. If needed, they can be retrieved from this storage to use for experiments.
Assessing Gas Production BioBricks in E.Coli
July 24
Notebook generator
July 25
Google Drive API
July 25
Automatic uploader
July 25
To be able to always get the necessary biobricks, we made an glycerol stock after every transformation. The glycerol stocks are stored at -80 degrees Celsius. If needed, they can retrieved from this storage to use for experiments.
Transformation
July 27
For further experiments there is isolated DNA needed of the biobricks J23100, K133071, K173003 and I134353. The DNA is isolated out of the bacteria with the help of a plasmid purifaction kit. After isolation this DNA can be used for digestion and ligation or other experiments. J23100 (from the glycerolstock): 325,59 ng/ul, K173003: 217,06 ng/ul, K133071: 186,79 ng/ul, I13453: 88,18 ng/ul
Assessing Gas Production BioBricks in E.Coli
July 27
Automation program
July 29
Automatic uploader
July 29
Page generator
July 31
Google Drive API
July 31
Automation program
July 31
Automation program
August 1
Notebook
August 2
For further experiments we needed isolated DNA. The DNA is isolated out of the bacteria with the help of a plasmid purifaction kit. After isolation this DNA can be used for digestion and ligation or other experiments.
Transformation
August 9
The biobricks J23100, K133071, K173003 and I134353 were sucessfully digested after the second time. After the digestions the biobricks K133071 and K173003 were dephosphorylated and ligated with the inserts J23100 and I13453. This was done in the original backbone of K133071 and K173003, and not another control backbone. To know if the biobricks were right ligated this was done by testing practically. See the experiments: Testing gas production.
Assessing Gas Production BioBricks in E.Coli
August 10
Testing different amounts of urea and sodiumpyruvate to know which concentrations the bacteria survive.
Urea and sodium pyruvate test for resistence E.coli
August 10
Transformations of the biobricks (K133071 + J23100), (K13071 + I1345), (K173003 + J23100) and (K173003 + I13453) in NEB10B�ta. There was no grow except for the biobrick combination K133071 + J23100.
Assessing Gas Production BioBricks in E.Coli
August 13
A colony PCR is done for the NEB10B�ta E.coli cells with expected the biobrick combination of K133071 with J23100. Nevertheless, on a gel the difference with and without promotor couldn't be seen. So there must be another way of proving the right biobricks are there.
Assessing Gas Production BioBricks in E.Coli
August 13
Assessing Gas Production BioBricks in E.Coli
August 13
Making a set up for the gas production testing and testing it with NEB10B�ta with the expected biobricks in it (K133071 + J23100) and a negative control.
Testing gas production
August 16
Making a set up for the gas production testing and testing it with NEB10B�ta with the expected biobricks in it (K133071 + I13453), (K173003 + J23100), (K173003 + I13453) and a negative control.
Testing gas production
August 23
Gasproduction testing for the biobricks (K173003 + I13453), (K133071 + I13453) and negative controls (B0015 and K133071 without urea and arabinose).
K173003 + I13453 is tested with sodiumpyruvate and arabinose for gasproduction and K133071 + I13453 is tested with urea and arabinose for gasproduction.
The negative control also produces a little bit gas.
Testing gas production
August 23
Testing the gasproduction of the colonies 9 and 10 of biobricks (K173003 + J23100), colonies 5 and 7 of biobricks (K173003 + I13453) and colony 4 of biobricks (K133071 + I13453) with and without centrifuging the bacteria. There is also a negative control (J04450 pSB1K3) with Kanamycine. The negative contol started with a lot of gas inside the tube. We can not see wether there is produced more after a day or not. This have to be tested later.
Testing gas production
August 23
Minipreps are made of colonies 11 and 19 of biobrick combination K133071 + J23100. Results: 11. 270,57 ng/ul 19. 253,59 ng/ul.
Assessing Gas Production BioBricks in E.Coli
August 27
A miniprep of the biobrick K352002 is made. The concentration is 69,77 ng/ul.
Assessing Gas Production BioBricks in E.Coli
August 27
Testing different amounts of urea and sodiumpyruvate to know which concentrations the bacteria survive.
Urea and sodium pyruvate test for resistence E.coli
August 28
Chemo competent cells
August 30
Digestions, gelelectrophoresis, dephosphorylations and ligations of different biobrick combinations. These biobrick combinations are 4 different promotors (J23100, I13453, K352002, K352003) with 4 different gasproduction biobricks (k173003, K173013, K133071, K133116). J23100 is about 1 kb to long. The rest seems likely to be right digested. The ligations will be transformed in NEB10b�ta and digested again as control.
Assessing Gas Production BioBricks in E.Coli
September 7
Transformations of the biobrick combinations in NEB10b�ta. The transformations are plated on kanamycine agar plates, because all the ligations were done in pSB1K3 (kanamycine resistence) backbone. Pink and with colonies appeared after incubation by 37 degrees Celcius for about 12h. The white colonies will be used for further experiments.
Assessing Gas Production BioBricks in E.Coli
September 7
Plasmid DNA was isolated of 20 different colonies. The first time something went wrong. The second time we had good concentrations of isolated plasmid DNA. After this we will digest the DNA to see if the plasmids all have the required biobricks. The tube have a code from now on, see table 1.
Number | Promotor | Gene | Backbone |
A | K352002 (CooF) | K173013 | pSB1K3 |
B | K352002 (CooF) | K133071 | pSB1K3 |
C | K352002 (CooF) | K173003 | pSB1K3 |
D | K352003 (CooM) | K133071 | pSB1K3 |
E | K352003 (CooM) | K173003 | pSB1K3 |
F | K352003 (CooM) | K173013 | pSB1K3 |
H | I13453 | K173013 | pSB1K3 |
I | I13453 | K173003 | pSB1K3 |
J | II13453 | K133071 | pSB1K3 |
Table 1: list of biobricks of abbreviations
Assessing Gas Production BioBricks in E.Coli
September 11
The minipreps of experiment 12 are digested with the restriction enzymes: SmaI and ScaI. Only Sca and Sma are both incubated at 37 degrees Celcius. Sma has to be incubated at 25 degrees Celcius. This is done in experiment 15.
Assessing Gas Production BioBricks in E.Coli
September 11
Digestions have been done of the biobrickcombinations K352002 +K133116 + pSB1K3 and K352003 + K133116 + pSB1K3 with restriction-enzymes ScaI and SmaI
Assessing Gas Production BioBricks in E.Coli
September 13
All the digestions of experiment 13 have digested again with SmaI for an our at 25 degrees Celcius. Because earlier the digestions have been put immediately bt 37 degrees Celcius. It looks like H1, J1 and D2 include the right biobricks.
Assessing Gas Production BioBricks in E.Coli
September 14
Minipreps of the biobrick combinations: K352002+K133116+pSB1K3 (1) and K352003+K133116+pSB1K3 (2) are done. The DNA concentration is for 1:189,3 ng/�l and for 2: 355,3 ng/�l.
Assessing Gas Production BioBricks in E.Coli
September 17
Different strains of E.coli are tested as negative control. Those strains were: NEB10B�ta, BL21 (DE3), BL21, HB101, DH5alpha and JM109. NEB10B�ta produced the most gas. BL21, HB101 and JM101 produced none/almost none gas. Because BL21 isn't a K12 strain we can not use that one. That is why we will test further gasproduction in HB101 and JM109. And maybe those will be our final E.coli strains.
Testing gas production
September 17
Assessing Gas Production BioBricks in E.Coli
September 20
Making overnight cultures of the numbers: A1, A4, A5, A6, B1, B5, B6 C1, C2, C3, C4, C5, C6, E1, E2, E5, E6, F4, F5 with Kanamycin. These biobrick combinations can be found in experiment 12. Also the first digestions with XbaI, Eco0109I, HindIII and SspI-HF have been done.
Assessing Gas Production BioBricks in E.Coli
September 27
Minipreps have been made of the numbers: A1, A4, A5, A6, B1, B5, B6 C1, C2, C3, C4, C5, C6, E1, E2, E5, E6, F4, F5.