Difference between revisions of "Team:SHSID China"

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<div class="splash" id="mainsplash" style="background: url(https://static.igem.org/mediawiki/2018/9/93/T--SHSID_China--main_bg.jpg); background-size: cover; background-position: center; position: relative" >
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<h1 style="font-family: 'Abril Fatface', cursive;">SHSID_CHINA</h1>
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            <h1 style="font-family: 'Abril Fatface', cursive;">InterLab</h1>
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<h2 style="font-family: 'Trocchi', serif;">On our way to a brighter future</h2>
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            <h2 style="font-family: 'Trocchi', serif;">Connecting labs across the globe.</h2>
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            <a href="#Abstract">
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                 <h3 style="color: white; font-family: 'Trocchi', serif;">Scroll down for more information</h3>
 
                 <h3 style="color: white; font-family: 'Trocchi', serif;">Scroll down for more information</h3>
 
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<div style="background: url(https://static.igem.org/mediawiki/2018/d/d6/T--SHSID_China--main_bg2.jpg); background-size: cover; background-position: center" class="link" id="Abstract">
 
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<h2 style="color: white; font-family: 'Trocchi', serif;">Project Description: Everglow</h2>
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        <h2 style="color: white; font-family: 'Trocchi', serif;">Introduction</h2>
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<p style="color: white; font-size: 16px; padding-top: 10px">With electricity consumption increasing across the globe, the conservation of energy has become a topic of major concern. Our team has devised an innovative solution to reduce electricity usage by attempting to create genetically modified bioluminescent plants. By altering particles on the microscopic level, we hope to create plants that can glow and thus replace electricity in the future. To these ends, our team conducted experiments to transfer the lux operon, a cluster of genes (LuxCDABEG) that control bioluminescence in the bacterial species <em>Aliivibrio fischeri</em>, to plant species like <em>Nicotiana tabacum</em>. We also attempted to insert an extra copy of LuxG to enhance the effects of bioluminescence. The results are promising and point to the possibility of creating a greener alternative to current lighting. Furthermore, we will design a new plasmid that can detect potential stress factors like ethanol and report the signal with stronger bioluminescence.</p>
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        <p style="color: white; font-size: 16px; padding-top: 10px">As in all of the natural sciences, repeatability and comparability of experiments and data are essential to future development. The same holds true for synthetic biology, where it is especially important, as labs require research from other labs to build off of. However, it is not uncommon for different labs to report measurements in different units, resulting in an inconvenient variability between labs. As a result, our team has decided to participate in the Fifth International InterLab Measurement Study, which aims to reduce systematic variability between labs. Specifically, we will be aiming to reduce variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of using optical density (OD).</p>
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<h2 style="color: white; font-family: 'Trocchi', serif;">A Video of Our Project</h2>
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        <h2 style="color: white; font-family: 'Trocchi', serif;">Plate Reader Details</h2>
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<iframe width="800" height="451" style="padding-top: 10px" src="https://www.youtube.com/embed/8jVF8hs2SFc" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
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        <ul style="color: white; font-size: 16px">
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                    <li>Instrument Brand and Model: Thermo Scientific Varioskan Flash 4.00.53</li>
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                    <li>Measurements: Supports both absorption and fluorescence</li>
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                    <li>Pathlength correction: Disabled</li>
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                    <li>Optics: Both top and bottom can be used</li>
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<h2 style="color: white; font-family: 'Trocchi', serif; padding-top:30px">Our Background</h2>
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                    <li>Temperature: Room temperature (around 25 degrees Celsius, varies slightly each time)</li>
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                    <li>Bandpass width: 12 nm</li>
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<p style="color: white; font-size: 16px; width: 41em;">We are SHSID_China, the first iGEM team from Shanghai High School International Division. Our story began in a small classroom on campus when sixteen seniors with a diverse range of skillsets and a common passion for synthetic Biology decided to work together to improve the environment around us. Aware of the environmental ramifications the high amounts of electric consumption used for lighting can have, our team began Project Everglow in hopes to engineer plants that can glow on their own. We hope that by inducing bioluminescence in plants, the need for electric lighting will decrease in the future. </p>
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                    <li>Excitation wavelength: 485 nm</li>
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                    <li>Emission wavelength: 520 nm</li>          
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<h2 style="color: white; font-family: 'Trocchi', serif; padding-top:30px">Accomplishments</h2>
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        <h2 style="color: white; font-family: 'Trocchi', serif;">Results</h2>
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        <h3 style="color: white; font-family: 'Trocchi', serif;">1. Calibration</h3>
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<li>Poster</li>
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        <table style="color: white; font-size: 16px">
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<li>Presentation at the Giant Jamboree in Boston</li>
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<li>Background research, design, and the creation of bioluminescent plants</li>
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<li>Worked with sponsors</li>
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                    <td></td>
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<li>Created and updated social media accounts</li>
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                    <td>LUDOX CL-X</td>
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<li>Collaboration with other iGEM Teams</li>
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                    <td>H2O</td>
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<li>Participation in the Measurement InterLab Study</li>
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                    <td>Replicate 1</td>
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                    <td>0.063189</td>
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<h2 style="color: white; font-family: 'Trocchi', serif; padding-bottom: 28px">Categories</h2>
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                    <td>0.043089</td>
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<div class="col-md-4 bg feat feat-left bg-team thumbnail" style="background:url(https://static.igem.org/mediawiki/2018/c/ca/T--SHSID_China--main_teambg.jpg); background-size: cover; background-position: center">
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                    <td>Replicate 2</td>
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                    <td>0.0633062</td>
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                    <td>0.045393</td>
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<big><big><big>Team</big></big></big><br>
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                    <td>Replicate 3</td>
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                    <td>0.0637365</td>
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                    <td>0.043681</td>
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<div class="col-md-4 bg feat feat-center bg-description thumbnail" style="background:url(https://static.igem.org/mediawiki/2018/4/4e/T--SHSID_China--main_projectbg.jpg); background-size: cover; background-position: center">
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                    <td>Replicate 4</td>
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<a href="https://2018.igem.org/Team:SHSID_China/Description">
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                    <td>0.064268</td>
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                    <td>0.044405</td>
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<big><big><big>Project</big></big></big><br>
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                    <td>Arith. Mean</td>
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                    <td>0.064</td>
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                    <td>0.044</td>
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                    <td>Corrected Abs600</td>
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                    <td>0.019</td>
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                    <td>Reference OD600</td>
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                    <td>0.063</td>
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                    <td>OD600/Abs600</td>
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                    <td>3.234</td>
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        <p style="color: white; font-size: 16px; padding-top: 10px; text-align: center">Table 1: Calibration results with our OD600/Abs600 conversion factor</p>
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        <p style="color: white; font-size: 16px; padding-top: 10px; text-align: center">According to the calibration test, our calculated OD600 to Abs600 conversion factor is 3.234.</p>
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        <h3 style="color: white; font-family: 'Trocchi', serif;">2. Particle Standard Curves</h3>
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        <h3 style="color: white; font-family: 'Trocchi', serif;">3. Fluorescein Standard Curves</h3>
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Revision as of 01:27, 15 October 2018

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