Difference between revisions of "Team:Ecuador/Model"
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<div class="ec--first--section"> | <div class="ec--first--section"> | ||
<div class="ec--main--title"> | <div class="ec--main--title"> | ||
| − | + | <a name="MOD" style="text-decoration: none;"><h1>Modeling</h1></a> | |
</div> | </div> | ||
<div class="ec--modeling--items"> | <div class="ec--modeling--items"> | ||
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<div class="ec--nau--content"> | <div class="ec--nau--content"> | ||
<div class="ec--sect--title"> | <div class="ec--sect--title"> | ||
| − | + | <a name="WWD" style="text-decoration: none;"><h2>WHAT DID WE DO?</h2></a> | |
<p>Parrafo<br></p> | <p>Parrafo<br></p> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img width="700" height="500" src=" | + | <p><img width="700" height="500" src="https://static.igem.org/mediawiki/2018/d/d6/T--ECUADOR--Modeling.jpeg"></p> |
</div> | </div> | ||
<h3>Converting between absorbance of cells to absorbance of a known concentration of beads.</h3> | <h3>Converting between absorbance of cells to absorbance of a known concentration of beads.</h3> | ||
| Line 518: | Line 518: | ||
<h3>Counting colony-forming units (CFUs) from the sample.</h3> | <h3>Counting colony-forming units (CFUs) from the sample.</h3> | ||
<p>…</p> | <p>…</p> | ||
| − | + | <a name="HWD" style="text-decoration: none;"><h2>HOW DID WE DO IT?</h2></a> | |
<p>In order to avoid distortions in the results due to differences in the experimentation processes, we follow the procedures described in the iGEM protocol (<a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">Plate reader protocol</a>, <a href="http://parts.igem.org/Help:Protocols/Competent_Cells">component_cells</a>, <a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation</a>) | <p>In order to avoid distortions in the results due to differences in the experimentation processes, we follow the procedures described in the iGEM protocol (<a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">Plate reader protocol</a>, <a href="http://parts.igem.org/Help:Protocols/Competent_Cells">component_cells</a>, <a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation</a>) | ||
<br> | <br> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | + | <a name="RES" style="text-decoration: none;"><h2>RESULTS</h2></a> | |
<strong>Particle standard curves</strong> | <strong>Particle standard curves</strong> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2018/3/30/T--ECUADOR--A-PSC.1.png" width="775" height="445" alt=""/></p> |
</div> | </div> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2018/1/1d/T--ECUADOR--A-PSC.2.png" width="779" height="445" alt=""/></p> |
</div> | </div> | ||
<br> | <br> | ||
<p><strong>Fluorescein standard curves</strong> </p> | <p><strong>Fluorescein standard curves</strong> </p> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2018/7/7c/T--ECUADOR--A-FSC.1.png" width="779" height="445" alt=""/><br></p> |
</div> | </div> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/c/cf/T--ECUADOR--A-FSC.2.png" width="779" height="445" alt=""/><br> |
</div> | </div> | ||
<strong>Net Absorbance 600 as number of particles/100 uL</strong></p> | <strong>Net Absorbance 600 as number of particles/100 uL</strong></p> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2018/6/64/T--ECUADOR--A-NA600.png" width="752" height="452" alt=""/></p> |
</div> | </div> | ||
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
| − | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2018/2/2e/T--ECUADOR--A-RBT.png" width="752" height="452" alt=""/></p> |
</div> | </div> | ||
| − | + | <a name="EXP" style="text-decoration: none;"><h2>EXPERIENCE</h2></a> | |
<div class="ec--img--wwd--cont"> | <div class="ec--img--wwd--cont"> | ||
<p><img src="https://svgsilh.com/svg/2324013-2196f3.svg" width="752" height="452" alt=""/></p> | <p><img src="https://svgsilh.com/svg/2324013-2196f3.svg" width="752" height="452" alt=""/></p> | ||
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<p><em>As a team the interlab challenge was useful because we learn about the protocols and the handling of the plate reader and how it can be used in later processes of our project</em></p> | <p><em>As a team the interlab challenge was useful because we learn about the protocols and the handling of the plate reader and how it can be used in later processes of our project</em></p> | ||
<br> | <br> | ||
| − | + | <a name="ACK" style="text-decoration: none;"><h2>ACKNOWLEDGEMENT</h2></a> | |
<br> | <br> | ||
As a team, we would like to thank the Biomedicine team of Universidad Tecnica Equinoccial and especially Linda Guaman, member of their research team, for allowing us to use their measurement equipment, which were essential for the completion of the Interlab.</div> | As a team, we would like to thank the Biomedicine team of Universidad Tecnica Equinoccial and especially Linda Guaman, member of their research team, for allowing us to use their measurement equipment, which were essential for the completion of the Interlab.</div> | ||
Revision as of 09:48, 8 October 2018
WHAT DID WE DO?
Parrafo
Converting between absorbance of cells to absorbance of a known concentration of beads.
...
Counting colony-forming units (CFUs) from the sample.
…
HOW DID WE DO IT?
In order to avoid distortions in the results due to differences in the experimentation processes, we follow the procedures described in the iGEM protocol (Plate reader protocol, component_cells, Transformation)
BIOBRICKS
Device |
Part Number |
Location |
Negative control |
BBa_R0040 |
Well 2D |
Positive control |
BBa_I20270 |
Well 2B |
Test Device 1 |
BBa_J364000 |
Well 2F |
Test Device 2 |
BBa_J364001 |
Well 2H |
Test Device 3 |
BBa_J364002 |
Well 2J |
Test Device 4 |
BBa_J364007 |
Well 2L |
Test Device 5 |
BBa_J364008 |
Well 2N |
Test Device 6 |
BBa_J364009 |
Well 2P |
Location
length: 54 bp
Location
length: 919 bp
Location
length: 918 bp
Location
length: 918 bp
Location
length: 918 bp
Location
length: 918 bp
Location
length: 918 bp
Location
length: 918 bp
RESULTS
Particle standard curves
Fluorescein standard curves
EXPERIENCE
As a team the interlab challenge was useful because we learn about the protocols and the handling of the plate reader and how it can be used in later processes of our project
ACKNOWLEDGEMENT
As a team, we would like to thank the Biomedicine team of Universidad Tecnica Equinoccial and especially Linda Guaman, member of their research team, for allowing us to use their measurement equipment, which were essential for the completion of the Interlab.