Difference between revisions of "Team:Tsinghua-A"

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                    <h2>Allergy Testing Master</h2>
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                    <p id="abstract">Histamine increases significantly in blood when allergy happens. So, we modifidied the pheromone pathway in the yeast to test histamine release in blood sample under one specific allergen. The pheromone receptor ste2 in original pathway is replaced by human histamine receptor H3 or H4. In order to reinforce the coupling between H3/H4 and yeast G-protein, C-terminal of α subunit of G-protein is modified by replacing several amino acids from the homologous protein in human. EGFP is pludged afterward the  promoter Fus1 as a reporter gene. Sufficient works substantiate our modifications. Then We construct models describing the relationship between histamine and EGFP intensity, which make contributions to the analysis of diagnosis credence.Besides, we design an integrated kit for the whole process of the blood collection, testing reaction and dignosis.Diagnosis result, after being calculated on our server, will be sent to terminals as smart phones, which makes our project possible for domestic uses.
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                     <h2><small>Project</small></h2>
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                         Collaboration
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                         <h3 align="center">Introduction</h3>
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                         With team Tsinghua
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                        <p> <br>This is a brief introduction for our projects in 2018</p>
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                    <p>July 20th, Li Yaqi and Yu Si, representatives of Team Tsinghua-A, along with main members of Team Tsinghua had a meet-up with Liu Dong, Chen Guoqiang and other professors of the School of Life Science. Design and difficulty in recent experiments fully discussed during the meeting, questions we raised answered respectively by professors, senior schoolmate willing to help, we benefited greatly as the bond between both teams strengthened.</p>
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                    <p>July 20th, Team Tsinghua-A borrowed TE buffer of Team Tsinghua’s lab.</p>
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                        <h4 align="center">Option one</h4>
  
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                    <p>August 3rd to 17th, both teams got visas in chorus, gave each other suggestions and set itinerary. We settled to attend the 5th CCIC (Conference of China iGEMer Community) in Shanghai.</p>
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                        <p><br>The biologic sensor part:</p>
  
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                    <p>September 20th to 30th, guided by professor Wang Xiaowo, Team Tsinghua-A got in contact with Zhang Lei, Tsinghua school mate and principal of China Science and Technology Museum. Member Guan Ai made draft plan of exhibition between October 4th to 7th in the museum. Delicate of team, Li Yaqi bargained with the museum and decided the final plan of exhibition. Afterwards, Huang Tianze, leader of Team Tsinghua proposed a joint exhibition plan with Team Tsinghua-A.</p>
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                        <p>We want to modify the  S. cerevisiae to produce the H3 or H4 histamine receptor which is supposed to be able to induce the pheromone responsive pathway instead of the autogenetic pheromone receptor ste2, as  the gene coding for ste2 have been knocked out. And we want to put fluorescent protein, like GFP or maybe another one after the special promoter, like fus1, promoted by pheromone responsive pathway, as our reporter gene.</p>
  
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                    <p>October 1st to 4th, Team Tsinhua-A borrowed E. coli strain which steadily express YFP, GFP and RFP fluoresce protein, and cosign it to Team Tsinghua to cultivate, and inoculate to solid medium.</p>
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                        <p>The electronic part:</p>
  
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                    <p>October 4th to 7th, exhibition in China Science and Technology Museum took place as arranged, daily watched by at least four members from Team Tsinghua-A and three members from Team Tsinghua.</p>
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                        <p>We want to make a reaction box to contain all of the reagents, and the biologic sensor part, the sum of the reporter fluorescent protein can be tested automatically and the information can be sent to the smart phone APP, and simple diagnosed can be made trough our pre-designed formulations.</p>
  
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                    <p>In the impending future, Team Tsinghua-A and Team Tsinghua should accomplish the final task in Giant Jamboree fabulously in joined hands.</p>
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                        <h4 align="center">Option two</h4>
  
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                        <p><br>we altered photosensitive E.coli to build a disposable info-reading system, which based on the light signal to induce GFP which will not be expressed immediately , and after the second light signal, the GFP will be expressed and be disposed. Our goal is to make a system which can achieve the function of disposable info-reading which can benefit in the secrecy and entertainment of kids.</p>
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                    <h2>Get <small>in touch</small></h2>
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                    <p> Feel free to contact with us for troubleshooting or collaboration</p>
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                        <li>Mentor E-mail: <a href="mailto:au_wang@foxmail.com">au_wang@foxmail.com</a></li>
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                        <li> E-mail: <a href="mailto:igem2018_thua@163.com">igem2018_thua@163.com</a> </li>
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                        <li> Phone: +86 010-62796050 </li>
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Revision as of 15:25, 10 October 2018

Allergy Testing Master

Histamine increases significantly in blood when allergy happens. So, we modifidied the pheromone pathway in the yeast to test histamine release in blood sample under one specific allergen. The pheromone receptor ste2 in original pathway is replaced by human histamine receptor H3 or H4. In order to reinforce the coupling between H3/H4 and yeast G-protein, C-terminal of α subunit of G-protein is modified by replacing several amino acids from the homologous protein in human. EGFP is pludged afterward the promoter Fus1 as a reporter gene. Sufficient works substantiate our modifications. Then We construct models describing the relationship between histamine and EGFP intensity, which make contributions to the analysis of diagnosis credence.Besides, we design an integrated kit for the whole process of the blood collection, testing reaction and dignosis.Diagnosis result, after being calculated on our server, will be sent to terminals as smart phones, which makes our project possible for domestic uses.

Project

Introduction


This is a brief introduction for our projects in 2018

Option one


The biologic sensor part:

We want to modify the S. cerevisiae to produce the H3 or H4 histamine receptor which is supposed to be able to induce the pheromone responsive pathway instead of the autogenetic pheromone receptor ste2, as the gene coding for ste2 have been knocked out. And we want to put fluorescent protein, like GFP or maybe another one after the special promoter, like fus1, promoted by pheromone responsive pathway, as our reporter gene.

The electronic part:

We want to make a reaction box to contain all of the reagents, and the biologic sensor part, the sum of the reporter fluorescent protein can be tested automatically and the information can be sent to the smart phone APP, and simple diagnosed can be made trough our pre-designed formulations.

Option two


we altered photosensitive E.coli to build a disposable info-reading system, which based on the light signal to induce GFP which will not be expressed immediately , and after the second light signal, the GFP will be expressed and be disposed. Our goal is to make a system which can achieve the function of disposable info-reading which can benefit in the secrecy and entertainment of kids.

Get in touch

Feel free to contact with us for troubleshooting or collaboration