Difference between revisions of "Team:Aalto-Helsinki/Demonstrate"

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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Revision as of 06:40, 12 October 2018


Demonstrate


Cloning

We assembled our constructs and cloned them into pSVA1 plasmid using Golden Gate assembly. After transforming plasmids to E.Coli Beta-10 and plasmid isolation we performed a check-up digestion with restriction enzymes NcoI and XhoI which cut the plasmid backbone close to BsaI insert site.


Protein expression and purification

We were able to successfully purify our silk containing construct prancerPurple-silk-CBM with the heat protocol


Cellulose binding

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Cellulose nanofibril binding

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Cotton binding

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Binding to hair keratin

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