Difference between revisions of "Team:NDC-HighRiverAB/Parts"
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<h1>PARTS</h1> | <h1>PARTS</h1> | ||
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| − | <p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality.</p> | + | |
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| + | <p>This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:</p> | ||
| + | <p> - First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in pseudomonas aeruginosa. (Part:BBa_K2694001) </> | ||
| + | <p> - For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011) </p> | ||
| + | <p> - The RBS we used is B0034. (Part:BBa_B0034) </p> | ||
| + | <p> - Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015) </p> | ||
| + | <p>- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000. | ||
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| + | </p> | ||
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<div> <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a> </div> | <div> <a href="http://parts.igem.org/Part:BBa_K2694000">BBa_K2694000</a> </div> | ||
<div> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">BBa_K2694001</a> </div> | <div> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2694001">BBa_K2694001</a> </div> | ||
</html> | </html> | ||
Revision as of 20:07, 12 October 2018
PARTS
This year our team created two new parts: a basic part and a composite part. Our basic part is EstA, our esterase gene. Our composite part drives expression of this part with a pLac promoter from the registry (BBa_R0011). We submitted our composite part to the registry and were able to characterize this part's functionality. In order to reach our goal of breaking down fats, which are a major component of buildups in wastewater systems, we selected the following biobrick parts for our plasmid with the goal of producing esterase:
- First, we researched an esterase-producing gene that could be expressed in E.coli. We decided to use the EstA gene, which is found in pseudomonas aeruginosa. (Part:BBa_K2694001)
- For our promoter, we decided to use pLAC, which is IPTG-inducible and is naturally found in E.coli. (Part:BBa_R0011)
- The RBS we used is B0034. (Part:BBa_B0034)
- Finally, we chose the double terminator B0015, which is the most common terminator and is known to be reliable. (Part:BBa_B0015)
- After we had chosen all of our biobrick parts, we combined them into the plasmid BBa_K2694000.