ATTRIBUTIONS

---

The Toulouse INSA-UPS iGEM team would like to thank all those who helped make this project come to life. It was not an easy ride but the help we received was better than what we could have hoped for.

General

---

First of all, let’s start by thanking our amazing PIs and advisors for being with us from day one. They helped us on so much more than the scientific part of our project!

Let’s not forget Coraline Rigouin who was our advisor for a time but unfortunately did not stay until the finish line.

Where would our project be today if we didn’t have a place to experiment? For providing this for three months, a big thank you to the Biochemical Engineering Department of INSA Toulouse.

To get the best results in biology, you need an extra dose of luck, and if it is not enough, equipment might be a must-have. For the latter, we would like to thank the LISBP, along with the GeT-Biopuce technological platform. They let us use their high-quality technological equipment such as their nano-dropper.

Finally, thanks to our predecessors, the 2017 iGEM Toulouse INSA-UPS team, for mentoring us during the first weeks and for always being there when we had questions.

Artwork

---

A big thank you to Xavier Flenner for spending time working on our logo.

Thanks to Vincent Hoffmann and Julien Hypolite for working again this year on the second version of our cardgame, Microbioworld.

Communication

---

Being students in the scientific field did not prepare us for the subtle arts of communication. Thus, we would like to thank Christelle Labruyère for advising us in this field, and the communication staff of our school (INSA Toulouse) and faculty (University Paul Sabatier) for giving us visibility.

Last, thanks to all the journalists who showed interest in our project and who wrote a newspaper article about us:

• Béatrice Colin (20 minutes)

• Lucas Hueber (La Dépêche)

• Xavier Terrien (Actu Toulouse)

Entrepreneurship

---

When we started our entrepreneurship approach, we received help from Guillaume Boissonat, the co-founder of a biotech start-up called pili.bio. He advised us on how to begin an entrepreneurship strategy and shared his experience as a start-up co-founder with us.

As our protein production began, we sought the help of Françoise Ouarne, the director of a biotech scale-up named CRITT-Bio-Industry, and she gave us advice on how to solve the problem of scaling up our production.

Finally, we collaborated with a business incubator named Le Catalyseur to build a strong entrepreneurship plan. For that, we were helped by Eric Marchadier, the director, Pierre-Emmanuel Hladik, the deputy director, and Nicolas Delcey, the incubator coordinator.

Human Practices

---

We met Cathy Metge and Aurélien Despierris at an Escape Game convention, and they are both teachers in a STL high school, a specialized formation focused on Science and Technology in Laboratory. Thanks to them and Philippe Garnier, an academic inspector that they introduced us to, we all worked together to find new ways to teach biotechnology by forming a collaboration between our team and the network of french high schools.

We intervened in a class composed of 35 CM1 (9 years old) children at the primary school of Belbèze in Toulouse thanks to the invitation of their school principal, Laurence Millard.

Thanks to David Leromain, innovation manager of the 1083 jean company, and René Jambon, director of the Prodhex company, for answering our questions about their perception of our project and how it could be used in the textile and medical industry.

During an event, we met David Faure who teaches at the International School of Toulouse. He was interested in our project and asked if we could present it in front of more than 200 students between the age of 11 to 19. To be able to present our project in front of such a broad public before the Giant Jamboree was a great experience for us. So, a big thank you to him for organising it, and to Carolyn Steinson, the school’s principal, and the other teachers for giving us the chance to be there.

Laboratory

---

The first one we want to thank is our spiritual mom: Sylvie Cancel-Pfister. She is a technician at the Biochemical Engineering Department of INSA Toulouse and she did her best to help us every time she could.

Thanks to Idaline Chanteperdrix, Philippe Serp, Laurent Ropiquet and Jérôme Volkman who work at the engineering school ENSIACET for helping us on the graphene and chemistry aspect of our project. They were kind enough to let us experiment in their laboratories and to lend us reactants such as graphene and carbon nanotubes.

For sharing his knowledge on the electroporator and for showing us how to use it, thanks to Thomas Lautier from the LISBP.

Thank you to Peter Schultz for sending us the Pichia pastoris GS200 strain, and two plasmids (pEVOL-AzF and pREAV-pFLD1-paPaRS).

Modelling

---

First, we would like to thank Jérémy Esque, Akli Ben Imeddourene, and Jelena Vucinic who are colleagues of our molecular modelling supervisor, Sophie Barbe, for helping and advising us during our project, especially for parametrising the ligands attached to our Cerberus and using VMD!

Thanks to CALMIP for providing us with 100,000 hours of calculation, and to Nicolas Renon and Mickaël Duval for training us.

Last, thanks to the Laboratory for Analysis and Architecture of Systems (LAAS) from the National Center for Scientific Research (CNRS) for exploring the intrinsically disordered regions of our protein with a robotics inspired AI. For further details, see our Modelling page.

Wiki

---

A huge thank you to Nathan Guiraud and Thibaud Laffargue for helping on the wiki during the last weeks before the wiki freeze. We really love you guys!