Difference between revisions of "Team:Grenoble-Alpes/components"

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<a  href="#lysis"><img style="margin-left: 25px; margin-right: 25px;" src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png"  alt="Bacterial lysis" title= "Bacterial lysis" /><h6>Bacterial lysis</h6></a>
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<a  href="#lysis"><img style="margin-left: 25px; margin-right: 25px;" src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png"  alt="Bacterial lysis" title= "Bacterial lysis" /></a>
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<a  href="#DNAb"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" /><h6>Bacterial lysis</h6></a>
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<a  href="#DNAb"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" title="DNA extraction"/></a>
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<a href="#probe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Probe design and digestion" /><h6>Bacterial lysis</h6></a>
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<a href="#probe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Probe design and digestion" title=Probe design and digestion /></a>
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<a href="#hybrid"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Hybridization" /><h6>Bacterial lysis</h6></a>
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<a href="#hybrid"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Hybridization" title="Hybridization"/></a>
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<a href="#transfo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Transformation" ><h6>Bacterial lysis</h6></a>
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<a href="#transfo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Transformation" title="Transformation" ></a>
 
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<a href="#autom"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Automation" /><h6>Bacterial lysis</h6></a>
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<a href="#autom"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Automation" title="Automation"/></a>
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<a href="#struct"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Structure" /><h6>Bacterial lysis</h6></a>
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<a href="#struct"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Structure" title="Structure" /></a>
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<a href="#DNAe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" /><h6>Bacterial lysis</h6></a>
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<a href="#DNAe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" title="DNA extraction" /></a>
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<a href="#Temp"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Temperature control" /></a>
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<a href="#Temp"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Temperature control" title="Temperature control" /></a>
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<a href="#Fluo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Fluorescence unit" /><h6>Bacterial lysis</h6></a>
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<a href="#Fluo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Fluorescence unit" title="Fluorescence unit" /></a>
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<a href="#elec"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Electronic components" /><h6>Bacterial lysis</h6></a>
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<a href="#elec"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Electronic components" title="Electronic components"/></a>
 
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Revision as of 19:01, 13 October 2018

Template loop detected: Template:Grenoble-Alpes

COMPONENTS

This page presents on one hand the cost of a single analysis in the biology part and on the other hand the cost of the device in the engineer part. You may click on the field you are interested in to access to the detailed amount per sector. The overall cost is given in $.

Biology

Total=$

Bacterial lysis DNA extraction Probe design and digestion Hybridization Transformation

Engineering

Total=$1281.84

Automation Structure DNA extraction Temperature control Fluorescence unit Electronic components

Bacterial lysis
Consumable Where can it be found to an affordable price? Price in $
Phages Laval university 300.00
Lysis buffer bioMerieux 0.23
Total 300.23
DNA extraction
Consumable Where can it be found to an affordable price? Price in $
easyMAG Magnetic Silica bioMerieux 0.03
easyMAG Buffer 1 bioMerieux 0.23
easyMAG Buffer 2 bioMerieux 0.31
easyMAG Buffer 3 bioMerieux 0.28
Total 0.85
Probe design and digestion
Consumable Where can it be found to an affordable price? Price in $
Nt.BspQI NEB 73.40
Nb.BsmI NEB 72.37
Nb.BssSI NEB 73.40
NgoMIV NEB 67.20
SphI-HF Promega 97.64
HaeIII Promega 65.48
RsaI Promega 56.29
Fsp NEB 69.27
Total XX
Hybridization
Consumable Where can it be found to an affordable price? Price in $
DNA probes / 0.00 (given by iGEM)
DNA / 0.00 (product of phage lysis)
Total 0.00
Transformation
Consumable Where can it be found to an affordable price? Price in $
TOP10 Home-made 0.00
DH5alpha Home-made 0.00
SOC Broth or LB Broth NEB 82.00
Total XX
Automation
Consumable Where can it be found to an affordable price? Price in $
Stepper motor Nema 17 (x2) eMotion Tech 32.30
Arduino Mega Arduino 40.37
Raspberry Pi 3B+ Raspberry Pi 42.41
Magnetic switch (x4) GoTronic 18.00
Neodymium magnet Supermagnete 1.40
Coupler (x2) eMotion Tech 11.30
Electronic pipette Second hand reseller 150.00
Total 295.78
Structure
Consumable Where can it be found to an affordable price? Price in $
Plexiglas Hardware shop 80.74
Delrin (for machining) Amazon 38.64 (given by bioMerieux)
PLA (for 3D printing) eMotion Tech 23.07 (given by Grenoble-INP Phelma)
Aluminum slot Hardware shop 51.95
Ball bearing (x3) RS-Online 22.49 (given by Grenoble-INP Phema)
Screw (M3 to M8) Hardware shop 57.67
Total 274.56
DNA extraction
Consumable Where can it be found to an affordable price? Price in $
Neodymium magnet Supermagnete 5.60
Actuator Actuonix 70.00 (given by bioMerieux)
Total 75.60
Temperature control
Consumable Where can it be found to an affordable price? Price in $
Cartridge heater (x4) Farnell 69.20
Peltier unit (x4) Farnell 344.68 (given by Phelma)
Thermal paste LDLC 17.30 (given by LDLC Grenoble)
Radiator + fan (x2) Amazon 34.60 (salvage)
Infrared sensor Digikey 27.30
Total 493.08
Fluorescence unit
Consumable Where can it be found to an affordable price? Price in $
Filter EdmundOptics 51.91
Smartphone magnifying lens Amazon 4.61
Picamera noIR v2 Raspberry Pi 25.00
565 nm LED (x8) Farnell 1.84
Total 83.36
Electronic components
Consumable Where can it be found to an affordable price? Price in $
Resistor (x19) RS-Online 11.02
DRV8825 pololu (x2) eMotion Tech 23.07
Chemical capacitor (x2) GoTronic 0.46
Polyester capacitor RS-Online 0.45
Bipolar transistor (x6) RS-Online 0.72
Transistor MOSFET (x3) RS-Online 1.61
Diode (x3) GoTronic 5.19
5V-3A power supply RS 4.61
12V supply Amazon 12.33
Total 59.46