Demonstrate

Gold Medal Criterion #4

XYLOSE AS FEEDSTOCK

✔Successfully constructed an inducible xylose-utilizing module

✔Demonstrated improved growth of E. coli BL21* containing this module in xylose and glucose-xylose mixture

DE NOVO BIOSYNTHESIS

✔Successfully constructed a naringenin-producing plasmid with just a single missing enzyme required for full de novo synthesis

✔Demonstrated production of naringenin from coumaric acid in E. coli Acella and BL21*

LUTEOLIN

✔Successfully constructed chemically-inducible and light inducible luteolin-producing plasmids

✔Demonstrated production of luteolin from naringenin in E. coli BL21*

✔Characterized Bba_ (F3’H), Bba (FNS), Bba (pBAD-FNS)

BLUE LIGHT REPRESSIBLE SYSTEM

✔Improved characterization of EL222 blue light repressible promoter, P_BLrep (BBa_)

✔Improved characterization of EL222 blue light inducible promoter, P_BLind (BBa_)

✔Demonstrated blue light repression of luteolin production

STRESS REPORTER

✔Successfully constructed a stress reporter module

✔Characterized the burden responsive promoter, P_htpG1

✔Demonstrated stress induced by naringenin- and luteolin-producing plasmids

CELL-MACHINE INTERFACE

✔Designed and built devices which help characterize optogenetic circuits in petri dishes and 250 ml conical flasks

✔Designed and built a 500 ml working volume benchtop optogenetic bioreactor, which comprises a peristaltic pump, 2-in-1 OD and fluorescence sensor, and fermentation chamber

✔Designed and implemented a feedback control system to control the optogenetic bioreactor