Difference between revisions of "Template:Groningen/Primers"

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     <h1>Sequences</h1>
 
     <h1>Sequences</h1>
 
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    <table width="100%"><tr><th>CRISPR-Cas Primers</th><th>sequence</th></tr><tr><td>588 FW</td><td>CTTGAAAACTTACGCTCATCTCTTCGACG</td></tr><tr><td>589 RV</td><td>CGGACTTAAGAAAATCCAGGATTGTCTTTCC</td></tr><tr><td>6006 FW</td><td>GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC</td></tr><tr><td>6005 RV</td><td>GATCATTTATCTTTCACTGCGGAGAAG</td></tr><tr><td>F ARO10 tar</td><td>TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCAATATACGAACGAAACAATGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC</td></tr><tr><td>R ARO10 tar</td><td>GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACCATTGTTTCGTTCGTATATTGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA</td></tr><tr><td>F AGA2 KO tar</td><td>TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCAAAACATACTGTGTGTTTATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC</td></tr><tr><td>R AGA2 KO tar</td><td>GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACATAAACACACAGTATGTTTTGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA</td></tr><tr><td>F AGA1 OE tar</td><td>TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCTTACGTTCATCTTTGAAAAAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC</td></tr><tr><td>R AGA1 OE tar</td><td>GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACTTTTTCAAAGATGAACGTAAGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA</td></tr><tr><td>CCasR1-RV</td><td>CGTGTAATCTGTGACAATTTCGATGCGTACACTGCGGCCCCGAAGTAGCATAGCTTATCTGGATCTGAACCTCGACTTTCTGGATCAGCC</td></tr><tr><td>CCasR2-FW</td><td>AGATAAGCTATGCTACTTCGGGGCCGCAGTGTACGCATCGAAATTGTCACAGATTACACGCGCTCTAGCATGGAACCAAGACCCATGACG</td></tr><tr><td>CCasR2-RV</td><td>TGAAATGTTATTATGTACCTCAGTACGCGCTAACATCCCTGGGTTACGCATCACTACATTGGATCTGAACCTCGACTTTCTGGATCAGCC</td></tr><tr><td>CCasR3-RV</td><td>TGTATCGAGCGATGTACGAATACTCTTGCCGAGTCATAGCAGTGCAGTAGAGCCTGACCCGGATCTGAACCTCGACTTTCTGGATCAGCC</td></tr><tr><td>CCasR3FW</td><td>AATGTAGTGATGCGTAACCCAGGGATGTTAGCGCGTACTGAGGTACATAATAACATTTCACGCTCTAGCATGGAACCAAGACCCATGACG</td></tr><tr><td>CCasR4-FW</td><td>GGGTCAGGCTCTACTGCACTGCTATGACTCGGCAAGAGTATTCGTACATCGCTCGATACACGCTCTAGCATGGAACCAAGACCCATGACG</td></tr><tr><td>CCasR5-FW</td><td>GCTCTCAACAGTCACTTGTGTGCCAAAATATTATACCTACTTCATACATTTTCAATTAAGCGCTCTAGCATGGAACCAAGACCCATGACG</td></tr><tr><td>CCasR5-RV</td><td>TAATCTTACACTAGTTTATAAGTGTCACGAACGATGAAATGGAAACTTGGGGGGGAAGGCGGATCTGAACCTCGACTTTCTGGATCAGCC</td></tr><tr><td>CCasR1-FW</td><td>ACAAGTTGACGCGACTTCTGTAAAGTTTATTTACAAGATAACAAAGAAACTCCCTTAAGCCGCTCTAGCATGGAACCAAGACCCATGACG</td></tr><tr><td>CCasR4-RV</td><td>TGTTAATGAACAGAAAACGAACAATTGGTAGCAGTGTTTTATAATTGCGCCCACAAGTTTGGATCTGAACCTCGACTTTCTGGATCAGCC</td></tr><tr><td>CCasAga1-FW</td><td>GCGCAGAATAATGATCTATCATCAATAATTATCGTGAACTTTTCCAGTATTTACGTTCATGCATGGAACCAAGACCCATGA</td></tr><tr><td>CCasAga1-RV</td><td>ATTAGTTAATCCCAACAATATTGTGAACAGGTAGGTAAAATGAGCGAAAGATAATGTCATACGAATGTACTAATTAAAAGATCAGAAAA</td></tr></table></div>
 
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    <table><tr><th>Cellulosome primers</th><th>sequence</th></tr><tr><td>BGLI-fragA-fw</td><td>TAGTAATGCAGACACTTGCG</td></tr><tr><td>BGLI-fragA-rv</td><td>CAACAGCGGTCTACTGAATC</td></tr><tr><td>BGLI-fragB-fw</td><td>CACTAGCTCAGATTCAGTAGACC</td></tr><tr><td>BGLI-fragB-rv</td><td>CGAGATGGACCGCAAG</td></tr><tr><td>BGLI1-FW</td><td>TTTGGATCCGTCTAGATGAA</td></tr><tr><td>BGL1-RV</td><td>AAACTCGAGTACTAGTATTC</td></tr><tr><td>BGL1A-FW</td><td>TTTGGATCCGTCTAGATGAGATTTCCTTCAATTTTTAC</td></tr><tr><td>BGL1A-RV</td><td>AAACTCGAGTACTAGTACTAATAAGGTAGGTGGGGTA</td></tr><tr><td>CBHII1-FW</td><td>TTTGGATCCGTCTAGATGAA</td></tr><tr><td>CBHII1-RV</td><td>AAACTCGAGTACTAGTAACT</td></tr><tr><td>CIPA3A-FW</td><td>TTTGGATCCGTCTAGATGCA</td></tr><tr><td>CIPA3A-RV</td><td>AAAGTCGACCTCGAGTACTA</td></tr><tr><td>CIPA3B-FW</td><td>TTTGGATCCGTCTAGATGCAGTTACTTCGCTGTTT</td></tr><tr><td>CIPA3B-RV</td><td>AAACTCGAGTACTAGTATTAGTGATGGTGATGGTGATGTTCGAAGGGCCCATTCGAAT</td></tr><tr><td>CIPA3C-RV</td><td>AAAGTCGACCTCGAGTATCA</td></tr><tr><td>EGII1-FW</td><td>TTTGGATCCGTCTAGATGAA</td></tr><tr><td>EGII1-RV</td><td>AAACTCGAGTACTAGTAGAC</td></tr><tr><td>Bio-His-EG-FW1</td><td>TATTTGAATTCGCGGCCGCTTCTAGATGCATCATCACCATCACCATG</td></tr><tr><td>Bio-His-EG-RV1</td><td>TATTTCTGCAGCGGCCGCTACTAGTATTACTTTCTTGCGAGACACGAG</td></tr><tr><td>Bio-ss1-HEGII-FW1</td><td>TATTTGAATTCGCGGCCGCTTCTAGATGAAGGTTTTGATTGTCTTG</td></tr><tr><td>Int-ss1-HEGII-FW1</td><td>TTTGGATCCGTCTAGATGATGAAGGTTTTGATTGTCTTG</td></tr><tr><td>Int-ss1-HEGII-RV1</td><td>AAACTCGAGTACTAGTATTACTTTCTTGCGAGACACGAGC</td></tr></table>
 
 
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    <table><tr><th>Sequencing primers</th><th>sequence</th></tr><tr><td>genseqFW</td><td>GCAGACGACTCGAATAAG</td></tr><tr><td>genseqRV</td><td>AGCTCGTCAATAGTGGAGATTAC</td></tr><tr><td>VR primer fw</td><td>ATTACCGCCTTTGAGTGAGC</td></tr><tr><td>VR primer rv</td><td>TGCCACCTGACGTCTAAGAA</td></tr><tr><td>M13 fw</td><td>GTAAAACGACGGCCAGT</td></tr><tr><td>M13 rv</td><td>CAGGAAACAGCTATGAC</td></tr><tr><td>EGII-seq-fw</td><td>GGCTGTACCACAGATGG</td></tr><tr><td>EGII-seq-rv</td><td>GTTCTGTCGGAGCCAAG</td></tr><tr><td>BGL1-seq-paper-FW</td><td>GCAACAGCCAAGCATTAC</td></tr><tr><td>BGL1-seq2-paper-FW</td><td>ACCACAGCGAGGTTATTC</td></tr><tr><td>PAL2-seq-ver-RV</td><td>GTGGTGCTCATCAGTTTC</td></tr><tr><td>PAL2-seq-fw-internal</td><td>CTGCGTGGCACCATTAC</td></tr></table>
 
 
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    <table><tr><th>Zeocin cassette primers</th><th>sequence</th></tr><tr><td>Zcas1-FW</td><td>TATTTGAATTCGCGGCCGCTTCTAGAGTCCAGAAAGTCGAGGTTCAG</td></tr><tr><td>Zcas2-FW</td><td>TATTTGAATTCGCGGCCGCTTCTAGAGGGGCTGATCCAGAAAGTCGA</td></tr><tr><td>Zcas3-FW</td><td>TATTTGAATTCGCGGCCGCTTCTAGAGCCAGAAAGTCGAGGTTCAG</td></tr><tr><td>Zcas4-FW</td><td>TATTTGAATTCGCGGCCGCTTCTAGAGGATCCAGAAAGTCGAGGTTCAG</td></tr><tr><td>Zcas1-RV</td><td>TATTTCTGCAGCGGCCGCTACTAGTATTATGCTTCCGGCTCGTATG</td></tr><tr><td>Zcas2-RV</td><td>TATTTCTGCAGCGGCCGCTACTAGTACTTCCGGCTCGTATGTTGTG</td></tr><tr><td>Zcas3-RV</td><td>TATTTCTGCAGCGGCCGCTACTAGTAGCTCGGAATTAACCCTCAC</td></tr></table>
 
 
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    <table><tr><th>Synthetic DNA sequences</th><th>Source</th></tr><tr><td>ss1-V5-EGII-DocS</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td></tr><tr><td>ss1-FLAG-CBHI-DocS</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td></tr><tr><td>ss1-myc-BGLI-DocA part1</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td></tr><tr><td>ss1-myc-BGLI-DocA part2</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td></tr><tr><td>AGA2-CIPA3-His part1</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td></tr><tr><td>AGA2-CIPA3-His part2</td><td>"Ordered from IDTA, designed based on Wen et al. 2010"</td><td></td></tr><tr><td>synthetic PAL2 gene part1</td><td>"Ordered from IDTA, designed based on McKenna, 2014"</td></tr><tr><td>synthetic PAL2 gene part2</td><td>"Ordered from IDTA, designed based on McKenna, 2014"</td></tr></table>
 
 
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    <table><tr><th>Strains</th><th>Source</th><th></th></tr><tr><td>BJ1991</td><td>"Molecular Cell Biology group</td><td>RUG"</td></tr><tr><td>BY4741</td><td>"Molmic group</td><td>RUG"</td></tr><tr><td>BJC3CB</td><td>This work</td><td></td></tr><tr><td>BJC1CB</td><td>This work</td><td></td></tr><tr><td>YPH499</td><td>"Molecular Cell Biology group</td><td>RUG"</td></tr><tr><td>YPHPAL2</td><td>This work</td><td></td></tr><tr><td>YPHCON</td><td>This work</td><td></td></tr></table>
 
 
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    <table><tr><th>Plasmids</th><th>Source</th></tr><tr><td>pYD1-CipA3-EGII</td><td>From Wen et al. 2010</td></tr><tr><td>pYD1-CipA1-EGII</td><td>From Wen et al. 2010</td></tr><tr><td>pRS425-CBHII-BGL1</td><td>From Wen et al. 2010</td></tr><tr><td>p414 KanMX TEF1-Cas9-Tcyc</td><td>"Molmic group, RUG"</td></tr><tr><td>pMEL16 OR1</td><td>"Molmic group, RUG"</td></tr><tr><td>pMEL16-ARO10tar</td><td>This work</td></tr><tr><td>pMEL16-AGA2tar</td><td>This work</td></tr><tr><td>pMEL16-AGA1tar</td><td>This work</td></tr><tr><td>pUC57-PAL2</td><td>"Roelfes group, RUG"</td></tr><tr><td>pRS313+ s promHxt7-MCS-terHxt7</td><td>"Molmic group, RUG"</td></tr><tr><td>pRS313-Hxt7-PAL2</td><td>This work</td></tr></table>
 
 
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Revision as of 17:22, 14 October 2018

Sequences